The Preyssler-Type Polyoxotungstate Exhibits Anti-Quorum Sensing, Antibiofilm, and Antiviral Activities

Detalhes bibliográficos
Autor(a) principal: Faleiro, Leonor
Data de Publicação: 2022
Outros Autores: Marques, Ana, Martins, João, Jordão, Luísa, Nogueira, Isabel, Gumerova, Nadiia I., Rompel, Annette, Aureliano, Manuel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/8437
Resumo: This article belongs to the Special Issue Metals and Metal Complexes in Diseases with a Focus on COVID-19: Facts and Opinions.
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spelling The Preyssler-Type Polyoxotungstate Exhibits Anti-Quorum Sensing, Antibiofilm, and Antiviral ActivitiesAntibacterial DrugsBacterial ResistanceAntibiotic ResistanceWells-Dawson PolyoxometalatesMultiresistantP5W30P2W18Resistência aos AntimicrobianosThis article belongs to the Special Issue Metals and Metal Complexes in Diseases with a Focus on COVID-19: Facts and Opinions.Simple Summary: Besides showing the antibacterial activity of the Preyssler-type polyoxotungstate (POT) P5W30 with the ability to affect MRSA cells, we demonstrated that P5W30 also displays other proprieties, such as anti-quorum sensing and antibiofilm. These are biological activities that are reported for a POT for the first time. Quorum sensing and biofilm facilitate the bacterial colonization, antibiotic resistance and persistence in both the environment and host, and its impairment by POTs can greatly contribute to the control of bacterial infections, such as those caused by multiresistant bacteria. Moreover, antiviral activity was also observed using the enterovirus Qβ. NMR stability studies of P5W30 demonstrate that it remains intact, suggesting its responsibility in the described biological activities. Taken together, our results emphasize the potential biomedical use of POTs, particularly the Preyssler-type POT, to fight antibiotic-resistant MRSA strains and their ability to form biofilm, besides being a promising antiviral agent.Abstract: The increase in bacterial resistance to antibiotics has led researchers to find new compounds or find combinations between different compounds with potential antibacterial action and with the ability to prevent the development of antibiotic resistance. Polyoxotungstates (POTs) are inorganic clusters that may fulfill that need, either individually or in combination with antibiotics. Herein, we report the ability of the polyoxotungstates (POTs) with Wells-Dawson P2W18, P2W17, P2W15, and Preyssler P5W30 type structures to differently affect Gram-negative and Gram-positive microorganisms, either susceptible or resistant to antibiotics. The compound P5W30 showed the highest activity against the majority of the tested bacterial strains in comparison with the other tested POTs (P2W15, P2W17 and P2W18) that did not show inhibition zones for the Gram-negative bacteria, A. baumanii I73775, E. coli DSM 1077, E. coli I73194, K. pneumoniae I7092374, and P. aeruginosa C46281). Generally, the results evidenced that Gram-positive bacteria are more susceptible to the POTs tested. The compound P5W30 was the one most active against S. aureus ATCC 6538 and MRSA16, reaching <0.83 mg·mL−1 (100 μM) and 4.96 mg·mL−1 (600 μM), respectively. Moreover, it was verified by NMR spectroscopy that the most promising POT, P5W30, remains intact under all the experimental conditions, after 24 h at 37 °C. This prompted us to further evaluate the anti-quorum sensing activity of P5W30 using the biosensor Chromobacterium violaceum CV026, as well as its antibiofilm activity both individually and in combination with the antibiotic cefoxitin against the methicillin-resistant Staphylococcus aureus 16 (MRSA16). P5W30 showed a synergistic antibacterial effect with the antibiotic cefoxitin and chloramphenicol against MRSA16. Moreover, the antibiofilm activity of P5W30 was more pronounced when used individually, in comparison with the combination with the antibiotic cefoxitin. Finally, the antiviral activity of P5W30 was tested using the coliphage Qβ, showing a dose-dependent response. The maximum inactivation was observed at 750 μM (6.23 mg·mL−1). In sum, P5W30 shows anti-quorum sensing and antibiofilm activities besides being a potent antibacterial agent against S. aureus and to exhibit antiviral activities against enteric viruses.This research was funded by FCT—Foundation for Science and Technology—through project UIDB/04326/2020 (M.A.) and LA/P/0101/2020; the Austrian Science Fund (FWF): P33927 (N.I.G.); P33089 (A.R.); and the University of Vienna (N.I.G. and A.R.). The authors are grateful to Algarve Biomedical Center (AD-ABC) for Open Access Funding.MDPIRepositório Científico do Instituto Nacional de SaúdeFaleiro, LeonorMarques, AnaMartins, JoãoJordão, LuísaNogueira, IsabelGumerova, Nadiia I.Rompel, AnnetteAureliano, Manuel2023-01-16T14:31:59Z2022-06-302022-06-30T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8437engBiology (Basel). 2022 Jun 30;11(7):994. doi: 10.3390/biology110709942079-773710.3390/biology11070994info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:28Zoai:repositorio.insa.pt:10400.18/8437Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:55.145681Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The Preyssler-Type Polyoxotungstate Exhibits Anti-Quorum Sensing, Antibiofilm, and Antiviral Activities
title The Preyssler-Type Polyoxotungstate Exhibits Anti-Quorum Sensing, Antibiofilm, and Antiviral Activities
spellingShingle The Preyssler-Type Polyoxotungstate Exhibits Anti-Quorum Sensing, Antibiofilm, and Antiviral Activities
Faleiro, Leonor
Antibacterial Drugs
Bacterial Resistance
Antibiotic Resistance
Wells-Dawson Polyoxometalates
Multiresistant
P5W30
P2W18
Resistência aos Antimicrobianos
title_short The Preyssler-Type Polyoxotungstate Exhibits Anti-Quorum Sensing, Antibiofilm, and Antiviral Activities
title_full The Preyssler-Type Polyoxotungstate Exhibits Anti-Quorum Sensing, Antibiofilm, and Antiviral Activities
title_fullStr The Preyssler-Type Polyoxotungstate Exhibits Anti-Quorum Sensing, Antibiofilm, and Antiviral Activities
title_full_unstemmed The Preyssler-Type Polyoxotungstate Exhibits Anti-Quorum Sensing, Antibiofilm, and Antiviral Activities
title_sort The Preyssler-Type Polyoxotungstate Exhibits Anti-Quorum Sensing, Antibiofilm, and Antiviral Activities
author Faleiro, Leonor
author_facet Faleiro, Leonor
Marques, Ana
Martins, João
Jordão, Luísa
Nogueira, Isabel
Gumerova, Nadiia I.
Rompel, Annette
Aureliano, Manuel
author_role author
author2 Marques, Ana
Martins, João
Jordão, Luísa
Nogueira, Isabel
Gumerova, Nadiia I.
Rompel, Annette
Aureliano, Manuel
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Faleiro, Leonor
Marques, Ana
Martins, João
Jordão, Luísa
Nogueira, Isabel
Gumerova, Nadiia I.
Rompel, Annette
Aureliano, Manuel
dc.subject.por.fl_str_mv Antibacterial Drugs
Bacterial Resistance
Antibiotic Resistance
Wells-Dawson Polyoxometalates
Multiresistant
P5W30
P2W18
Resistência aos Antimicrobianos
topic Antibacterial Drugs
Bacterial Resistance
Antibiotic Resistance
Wells-Dawson Polyoxometalates
Multiresistant
P5W30
P2W18
Resistência aos Antimicrobianos
description This article belongs to the Special Issue Metals and Metal Complexes in Diseases with a Focus on COVID-19: Facts and Opinions.
publishDate 2022
dc.date.none.fl_str_mv 2022-06-30
2022-06-30T00:00:00Z
2023-01-16T14:31:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/8437
url http://hdl.handle.net/10400.18/8437
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biology (Basel). 2022 Jun 30;11(7):994. doi: 10.3390/biology11070994
2079-7737
10.3390/biology11070994
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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