Sialyl LewisX/A and Cytokeratin Crosstalk in Triple Negative Breast Cancer

Detalhes bibliográficos
Autor(a) principal: Pascoal, Carlota
Data de Publicação: 2023
Outros Autores: Carrascal, Mylène A., Barreira, Daniela F., Lourenço, Rita A., Granjo, Pedro, Grosso, Ana R., Borralho, Paula, Braga, Sofia, Videira, Paula A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/151539
Resumo: project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. Publisher Copyright: © 2023 by the authors.
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spelling Sialyl LewisX/A and Cytokeratin Crosstalk in Triple Negative Breast Canceraberrant glycosylationcytokeratin expressiondisease-free survival rateintermediate filament proteinssialyl Lewis (sLe)triple-negative breast cancer (TNBC)α6 integrinOncologyCancer ResearchSDG 3 - Good Health and Well-beingproject LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. Publisher Copyright: © 2023 by the authors.Triple-negative breast cancer (TNBC) encompasses multiple entities and is generally highly aggressive and metastatic. We aimed to determine the clinical and biological relevance of Sialyl-Lewis X and A (sLeX/A)—a fucosylated glycan involved in metastasis—in TNBC. Here, we studied tissues from 50 TNBC patients, transcripts from a TNBC dataset from The Cancer Genome Atlas (TCGA) database, and a primary breast cancer cell line. All 50 TNBC tissue samples analysed expressed sLeX/A. Patients with high expression of sLeX/A had 3 years less disease-free survival than patients with lower expression. In tissue, sLeX/A negatively correlated with cytokeratins 5/6 (CK5/6, which was corroborated by the inverse correlation between fucosyltransferases and CK5/6 genes. Our observations were confirmed in vitro when inhibition of sLeX/A remarkably increased expression of CK5/6, followed by a decreased proliferation and invasion capacity. Among the reported glycoproteins bearing sLeX/A and based on the STRING tool, α6 integrin showed the highest interaction score with CK5/6. This is the first report on the sLeX/A expression in TNBC, highlighting its association with lower disease-free survival and its inverse crosstalk with CK5/6 with α6 integrin as a mediator. All in all, sLeX/A is critical for TNBC malignancy and a potential prognosis biomarker and therapeutic target.UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNPascoal, CarlotaCarrascal, Mylène A.Barreira, Daniela F.Lourenço, Rita A.Granjo, PedroGrosso, Ana R.Borralho, PaulaBraga, SofiaVideira, Paula A.2023-04-03T22:14:14Z2023-022023-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15application/pdfhttp://hdl.handle.net/10362/151539engPascoal, C., Carrascal, M. A., Barreira, D. F., Lourenço, R. A., Granjo, P., Grosso, A. R., Borralho, P., Braga, S., & Videira, P. A. (2023). Sialyl LewisX/A and Cytokeratin Crosstalk in Triple Negative Breast Cancer. Cancers, 15(3), [731]. https://doi.org/10.3390/cancers150307312072-6694PURE: 54944853https://doi.org/10.3390/cancers15030731info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:33:57Zoai:run.unl.pt:10362/151539Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:54:37.423804Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Sialyl LewisX/A and Cytokeratin Crosstalk in Triple Negative Breast Cancer
title Sialyl LewisX/A and Cytokeratin Crosstalk in Triple Negative Breast Cancer
spellingShingle Sialyl LewisX/A and Cytokeratin Crosstalk in Triple Negative Breast Cancer
Pascoal, Carlota
aberrant glycosylation
cytokeratin expression
disease-free survival rate
intermediate filament proteins
sialyl Lewis (sLe)
triple-negative breast cancer (TNBC)
α6 integrin
Oncology
Cancer Research
SDG 3 - Good Health and Well-being
title_short Sialyl LewisX/A and Cytokeratin Crosstalk in Triple Negative Breast Cancer
title_full Sialyl LewisX/A and Cytokeratin Crosstalk in Triple Negative Breast Cancer
title_fullStr Sialyl LewisX/A and Cytokeratin Crosstalk in Triple Negative Breast Cancer
title_full_unstemmed Sialyl LewisX/A and Cytokeratin Crosstalk in Triple Negative Breast Cancer
title_sort Sialyl LewisX/A and Cytokeratin Crosstalk in Triple Negative Breast Cancer
author Pascoal, Carlota
author_facet Pascoal, Carlota
Carrascal, Mylène A.
Barreira, Daniela F.
Lourenço, Rita A.
Granjo, Pedro
Grosso, Ana R.
Borralho, Paula
Braga, Sofia
Videira, Paula A.
author_role author
author2 Carrascal, Mylène A.
Barreira, Daniela F.
Lourenço, Rita A.
Granjo, Pedro
Grosso, Ana R.
Borralho, Paula
Braga, Sofia
Videira, Paula A.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DCV - Departamento de Ciências da Vida
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Pascoal, Carlota
Carrascal, Mylène A.
Barreira, Daniela F.
Lourenço, Rita A.
Granjo, Pedro
Grosso, Ana R.
Borralho, Paula
Braga, Sofia
Videira, Paula A.
dc.subject.por.fl_str_mv aberrant glycosylation
cytokeratin expression
disease-free survival rate
intermediate filament proteins
sialyl Lewis (sLe)
triple-negative breast cancer (TNBC)
α6 integrin
Oncology
Cancer Research
SDG 3 - Good Health and Well-being
topic aberrant glycosylation
cytokeratin expression
disease-free survival rate
intermediate filament proteins
sialyl Lewis (sLe)
triple-negative breast cancer (TNBC)
α6 integrin
Oncology
Cancer Research
SDG 3 - Good Health and Well-being
description project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. Publisher Copyright: © 2023 by the authors.
publishDate 2023
dc.date.none.fl_str_mv 2023-04-03T22:14:14Z
2023-02
2023-02-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/151539
url http://hdl.handle.net/10362/151539
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pascoal, C., Carrascal, M. A., Barreira, D. F., Lourenço, R. A., Granjo, P., Grosso, A. R., Borralho, P., Braga, S., & Videira, P. A. (2023). Sialyl LewisX/A and Cytokeratin Crosstalk in Triple Negative Breast Cancer. Cancers, 15(3), [731]. https://doi.org/10.3390/cancers15030731
2072-6694
PURE: 54944853
https://doi.org/10.3390/cancers15030731
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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