Saliva molecular testing bypassing RNA extraction is suitable for monitoring and diagnosing SARS-CoV-2 infection in children
| Autor(a) principal: | |
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| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/142070 |
Resumo: | Funding Information: This work was funded by the Fundação para a Ciência and Tecnologia (FCT, Portugal) under RESEARCH4COVID 19 call with reference 283_596885654 and co-funded by ANI under INOV4COVID (Funding to V.M.B.). M.J.A. is funded by the FCT (CEECIND/02373/2020). M.A. is funded by a Junior Researcher working contract from FCT and Instituto Gulbenkian de Ciência (IGC, Portugal). This work benefited from COVID19 emergency funds 2020 from Calouste Gulbenkian Foundation and from Oeiras city council. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We would like to thank the technical support of IGC’s Advanced Imaging Facility (AIF-UIC). |
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Saliva molecular testing bypassing RNA extraction is suitable for monitoring and diagnosing SARS-CoV-2 infection in childrenGeneralSDG 3 - Good Health and Well-beingFunding Information: This work was funded by the Fundação para a Ciência and Tecnologia (FCT, Portugal) under RESEARCH4COVID 19 call with reference 283_596885654 and co-funded by ANI under INOV4COVID (Funding to V.M.B.). M.J.A. is funded by the FCT (CEECIND/02373/2020). M.A. is funded by a Junior Researcher working contract from FCT and Instituto Gulbenkian de Ciência (IGC, Portugal). This work benefited from COVID19 emergency funds 2020 from Calouste Gulbenkian Foundation and from Oeiras city council. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We would like to thank the technical support of IGC’s Advanced Imaging Facility (AIF-UIC).Background Adults are being vaccinated against SARS-CoV-2 worldwide, but the longitudinal protection of these vaccines is uncertain, given the ongoing appearance of SARS-CoV-2 variants. Children remain largely unvaccinated and are susceptible to infection, with studies reporting that they actively transmit the virus even when asymptomatic, thus affecting the community. Methods We investigated if saliva is an effective sample for detecting SARS-CoV-2 RNA and antibodies in children, and associated viral RNA levels to infectivity. For that, we used a saliva-based SARS-CoV-2 RT-qPCR test, preceded or not by RNA extraction, in 85 children aged 10 years and under, admitted to the hospital regardless of COVID-19 symptomatology. Amongst these, 29 (63.0%) presented at least one COVID-19 symptom, 46 (54.1%) were positive for SARS-CoV-2 infection, 28 (32.9%) were under the age of 1, and the mean (SD) age was 3.8 (3.4) years. Saliva samples were collected up to 48 h after a nasopharyngeal swab-RT-qPCR test. Results In children aged 10 years and under, the sensitivity, specificity, and accuracy of saliva-RT-qPCR tests compared to NP swab-RT-qPCR were, respectively, 84.8% (71.8%–92.4%), 100% (91.0%–100%), and 91.8% (84.0%–96.6%) with RNA extraction, and 81.8% (68.0%–90.5%), 100% (91.0%–100%), and 90.4% (82.1%–95.0%) without RNA extraction. Rescue of infectious particles from saliva was limited to CT values below 26. In addition, we found significant IgM positive responses to SARS-CoV-2 in children positive for SARS-CoV-2 by NP swab and negative by saliva compared to other groups, indicating late infection onset (>7–10 days). Conclusions Saliva is a suitable sample type for diagnosing children aged 10 years and under, including infants aged <1 year, even bypassing RNA extraction methods. Importantly, the detected viral RNA levels were significantly above the infectivity threshold in several samples. Further investigation is required to correlate SARS-CoV-2 RNA levels to viral transmission.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)RUNAlenquer, MartaSilva, Tiago MilheiroAkpogheneta, OnomeFerreira, FilipeVale-Costa, SílviaMedina-Lopes, MónicaBatista, FredericoGarcia, Ana MargaridaBarreto, Vasco M.Paulino, CathyCosta, JoãoSobral, JoãoDiniz-Da-Costa, MariaLadeiro, SusanaCorte-Real, RitaAlves, José DelgadoLeite, Ricardo B.Demengeot, JocelyneBrito, Maria João RochaAmorim, Maria João2022-07-18T22:27:08Z2022-062022-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/142070eng1932-6203PURE: 45372087https://doi.org/10.1371/journal.pone.0268388info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T18:03:32Zoai:run.unl.pt:10362/142070Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T18:03:32Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Saliva molecular testing bypassing RNA extraction is suitable for monitoring and diagnosing SARS-CoV-2 infection in children |
| title |
Saliva molecular testing bypassing RNA extraction is suitable for monitoring and diagnosing SARS-CoV-2 infection in children |
| spellingShingle |
Saliva molecular testing bypassing RNA extraction is suitable for monitoring and diagnosing SARS-CoV-2 infection in children Alenquer, Marta General SDG 3 - Good Health and Well-being |
| title_short |
Saliva molecular testing bypassing RNA extraction is suitable for monitoring and diagnosing SARS-CoV-2 infection in children |
| title_full |
Saliva molecular testing bypassing RNA extraction is suitable for monitoring and diagnosing SARS-CoV-2 infection in children |
| title_fullStr |
Saliva molecular testing bypassing RNA extraction is suitable for monitoring and diagnosing SARS-CoV-2 infection in children |
| title_full_unstemmed |
Saliva molecular testing bypassing RNA extraction is suitable for monitoring and diagnosing SARS-CoV-2 infection in children |
| title_sort |
Saliva molecular testing bypassing RNA extraction is suitable for monitoring and diagnosing SARS-CoV-2 infection in children |
| author |
Alenquer, Marta |
| author_facet |
Alenquer, Marta Silva, Tiago Milheiro Akpogheneta, Onome Ferreira, Filipe Vale-Costa, Sílvia Medina-Lopes, Mónica Batista, Frederico Garcia, Ana Margarida Barreto, Vasco M. Paulino, Cathy Costa, João Sobral, João Diniz-Da-Costa, Maria Ladeiro, Susana Corte-Real, Rita Alves, José Delgado Leite, Ricardo B. Demengeot, Jocelyne Brito, Maria João Rocha Amorim, Maria João |
| author_role |
author |
| author2 |
Silva, Tiago Milheiro Akpogheneta, Onome Ferreira, Filipe Vale-Costa, Sílvia Medina-Lopes, Mónica Batista, Frederico Garcia, Ana Margarida Barreto, Vasco M. Paulino, Cathy Costa, João Sobral, João Diniz-Da-Costa, Maria Ladeiro, Susana Corte-Real, Rita Alves, José Delgado Leite, Ricardo B. Demengeot, Jocelyne Brito, Maria João Rocha Amorim, Maria João |
| author2_role |
author author author author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Centro de Estudos de Doenças Crónicas (CEDOC) RUN |
| dc.contributor.author.fl_str_mv |
Alenquer, Marta Silva, Tiago Milheiro Akpogheneta, Onome Ferreira, Filipe Vale-Costa, Sílvia Medina-Lopes, Mónica Batista, Frederico Garcia, Ana Margarida Barreto, Vasco M. Paulino, Cathy Costa, João Sobral, João Diniz-Da-Costa, Maria Ladeiro, Susana Corte-Real, Rita Alves, José Delgado Leite, Ricardo B. Demengeot, Jocelyne Brito, Maria João Rocha Amorim, Maria João |
| dc.subject.por.fl_str_mv |
General SDG 3 - Good Health and Well-being |
| topic |
General SDG 3 - Good Health and Well-being |
| description |
Funding Information: This work was funded by the Fundação para a Ciência and Tecnologia (FCT, Portugal) under RESEARCH4COVID 19 call with reference 283_596885654 and co-funded by ANI under INOV4COVID (Funding to V.M.B.). M.J.A. is funded by the FCT (CEECIND/02373/2020). M.A. is funded by a Junior Researcher working contract from FCT and Instituto Gulbenkian de Ciência (IGC, Portugal). This work benefited from COVID19 emergency funds 2020 from Calouste Gulbenkian Foundation and from Oeiras city council. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We would like to thank the technical support of IGC’s Advanced Imaging Facility (AIF-UIC). |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-07-18T22:27:08Z 2022-06 2022-06-01T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10362/142070 |
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http://hdl.handle.net/10362/142070 |
| dc.language.iso.fl_str_mv |
eng |
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eng |
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1932-6203 PURE: 45372087 https://doi.org/10.1371/journal.pone.0268388 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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mluisa.alvim@gmail.com |
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