Adenosine A3 receptors in the rat hippocampus: Lack of interaction with A1 receptors
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/8431 https://doi.org/10.1002/ddr.10188 |
Resumo: | Adenosine acts as a neuromodulator in the hippocampus essentially through activation of inhibitory A1 receptors. Using single-cell PCR analysis, we found that CA1 pyramidal cells coexpress A1 receptor mRNA together with that of another adenosine receptor, the A3 receptor. As occurs for the A1 receptor, Western blot analysis indicated that the A3 receptor is also located in hippocampal nerve terminals. However, activation of A3 receptors with its purportedly selective agonist Cl-IBMECA (0.1-10 µM) failed to affect hippocampal synaptic transmission or to modify the evoked release of glutamate or GABA. Also, blockade of A3 receptors with MRS 1191 (5 µM) failed to affect either hypoxia- or ischemia-induced depression of hippocampal synaptic transmission. Activation of A3 receptors also failed to control A1 receptor function, as Cl-IBMECA (100 nM) did not modify the ability of CPA to displace [3H]DPCPX binding to hippocampal membranes or the A1 receptor-mediated inhibition of hippocampal synaptic transmission. However, ligand binding studies revealed that Cl-IBMECA displaced the binding of an A1 receptor agonist ([3H]R-PIA, Ki=47 nM) or antagonist ([3H]DPCPX, Ki=130 nM), which suggests that A3 receptor ligands also act on native A1 receptors. We believe that A3 receptors are expressed in hippocampal neurons and are located in hippocampal nerve terminals, though their function remains elusive. Drug Dev. Res. 58:428-438, 2003. © 2003 Wiley-Liss, Inc. |
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Adenosine A3 receptors in the rat hippocampus: Lack of interaction with A1 receptorsAdenosine acts as a neuromodulator in the hippocampus essentially through activation of inhibitory A1 receptors. Using single-cell PCR analysis, we found that CA1 pyramidal cells coexpress A1 receptor mRNA together with that of another adenosine receptor, the A3 receptor. As occurs for the A1 receptor, Western blot analysis indicated that the A3 receptor is also located in hippocampal nerve terminals. However, activation of A3 receptors with its purportedly selective agonist Cl-IBMECA (0.1-10 µM) failed to affect hippocampal synaptic transmission or to modify the evoked release of glutamate or GABA. Also, blockade of A3 receptors with MRS 1191 (5 µM) failed to affect either hypoxia- or ischemia-induced depression of hippocampal synaptic transmission. Activation of A3 receptors also failed to control A1 receptor function, as Cl-IBMECA (100 nM) did not modify the ability of CPA to displace [3H]DPCPX binding to hippocampal membranes or the A1 receptor-mediated inhibition of hippocampal synaptic transmission. However, ligand binding studies revealed that Cl-IBMECA displaced the binding of an A1 receptor agonist ([3H]R-PIA, Ki=47 nM) or antagonist ([3H]DPCPX, Ki=130 nM), which suggests that A3 receptor ligands also act on native A1 receptors. We believe that A3 receptors are expressed in hippocampal neurons and are located in hippocampal nerve terminals, though their function remains elusive. Drug Dev. Res. 58:428-438, 2003. © 2003 Wiley-Liss, Inc.2003info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/8431http://hdl.handle.net/10316/8431https://doi.org/10.1002/ddr.10188engDrug Development Research. 58:4 (2003) 428-438Lopes, Luísa V.Rebola, NelsonCostenla, Ana RitaHalldner, LindaJacobson, Marlene A.Oliveira, Catarina R.Richardson, Peter J.Fredholm, Bertil B.Ribeiro, Joaquim A.Cunha, Rodrigo A.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-05-27T16:14:32Zoai:estudogeral.uc.pt:10316/8431Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:43:32.234463Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Adenosine A3 receptors in the rat hippocampus: Lack of interaction with A1 receptors |
title |
Adenosine A3 receptors in the rat hippocampus: Lack of interaction with A1 receptors |
spellingShingle |
Adenosine A3 receptors in the rat hippocampus: Lack of interaction with A1 receptors Lopes, Luísa V. |
title_short |
Adenosine A3 receptors in the rat hippocampus: Lack of interaction with A1 receptors |
title_full |
Adenosine A3 receptors in the rat hippocampus: Lack of interaction with A1 receptors |
title_fullStr |
Adenosine A3 receptors in the rat hippocampus: Lack of interaction with A1 receptors |
title_full_unstemmed |
Adenosine A3 receptors in the rat hippocampus: Lack of interaction with A1 receptors |
title_sort |
Adenosine A3 receptors in the rat hippocampus: Lack of interaction with A1 receptors |
author |
Lopes, Luísa V. |
author_facet |
Lopes, Luísa V. Rebola, Nelson Costenla, Ana Rita Halldner, Linda Jacobson, Marlene A. Oliveira, Catarina R. Richardson, Peter J. Fredholm, Bertil B. Ribeiro, Joaquim A. Cunha, Rodrigo A. |
author_role |
author |
author2 |
Rebola, Nelson Costenla, Ana Rita Halldner, Linda Jacobson, Marlene A. Oliveira, Catarina R. Richardson, Peter J. Fredholm, Bertil B. Ribeiro, Joaquim A. Cunha, Rodrigo A. |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lopes, Luísa V. Rebola, Nelson Costenla, Ana Rita Halldner, Linda Jacobson, Marlene A. Oliveira, Catarina R. Richardson, Peter J. Fredholm, Bertil B. Ribeiro, Joaquim A. Cunha, Rodrigo A. |
description |
Adenosine acts as a neuromodulator in the hippocampus essentially through activation of inhibitory A1 receptors. Using single-cell PCR analysis, we found that CA1 pyramidal cells coexpress A1 receptor mRNA together with that of another adenosine receptor, the A3 receptor. As occurs for the A1 receptor, Western blot analysis indicated that the A3 receptor is also located in hippocampal nerve terminals. However, activation of A3 receptors with its purportedly selective agonist Cl-IBMECA (0.1-10 µM) failed to affect hippocampal synaptic transmission or to modify the evoked release of glutamate or GABA. Also, blockade of A3 receptors with MRS 1191 (5 µM) failed to affect either hypoxia- or ischemia-induced depression of hippocampal synaptic transmission. Activation of A3 receptors also failed to control A1 receptor function, as Cl-IBMECA (100 nM) did not modify the ability of CPA to displace [3H]DPCPX binding to hippocampal membranes or the A1 receptor-mediated inhibition of hippocampal synaptic transmission. However, ligand binding studies revealed that Cl-IBMECA displaced the binding of an A1 receptor agonist ([3H]R-PIA, Ki=47 nM) or antagonist ([3H]DPCPX, Ki=130 nM), which suggests that A3 receptor ligands also act on native A1 receptors. We believe that A3 receptors are expressed in hippocampal neurons and are located in hippocampal nerve terminals, though their function remains elusive. Drug Dev. Res. 58:428-438, 2003. © 2003 Wiley-Liss, Inc. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/8431 http://hdl.handle.net/10316/8431 https://doi.org/10.1002/ddr.10188 |
url |
http://hdl.handle.net/10316/8431 https://doi.org/10.1002/ddr.10188 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Drug Development Research. 58:4 (2003) 428-438 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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