Acute effects of insulin and insulin-induced hypoglycaemia on carotid body chemoreceptor activity and cardiorespiratory responses in dogs

Detalhes bibliográficos
Autor(a) principal: Baby, Santhosh M.
Data de Publicação: 2022
Outros Autores: Zaidi, Faisal, Hunsberger, Gerald E., Sokal, David, Gupta, Isha, Conde, Silvia V., Chew, Daniel, Rall, Kristen, Coatney, Robert W.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/146187
Resumo: Funding: Silvia V. Conde declares that Galvani Bioelectronics provided funds to support their work associated with Type 2 diabetic project.
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spelling Acute effects of insulin and insulin-induced hypoglycaemia on carotid body chemoreceptor activity and cardiorespiratory responses in dogscarotid bodycarotid body chemoreceptorsdogshypoglycaemiainsulinsodium cyanidePhysiologyNutrition and DieteticsPhysiology (medical)Funding: Silvia V. Conde declares that Galvani Bioelectronics provided funds to support their work associated with Type 2 diabetic project.New Findings: What is the central question of this study? What are the effects of insulin and insulin-induced hypoglycaemia on carotid body chemoreceptor activity in vivo and how do carotid body chemoreceptor stimulation-mediated cardiorespiratory responses in beagle dogs compare during euglycaemia and insulin-induced hypoglycaemia? What is the main finding and its importance? Intracarotid insulin administration leads to sustained increase in carotid body chemoreceptor activity and respiratory response with significant cardiovascular effects. Insulin-induced hypoglycaemia exacerbated NaCN-mediated carotid body chemoreceptor activity and respiratory response with enhanced cardiovascular reflex response. These findings suggest that insulin-induced hypoglycaemia augments the carotid body chemoreceptors to initiate the adaptive counter-regulatory responses to restore the normoglycaemic condition. Abstract: The carotid body chemoreceptors (CBC) play an important role in the adaptive counter-regulatory response to hypoglycaemia by evoking the CBC-mediated sympathetic neuronal system to restore normoglycaemia. Ex vivo studies have shown varied responses of insulin-induced hypoglycaemia on CBC function, and several in vivo studies have indirectly established the role of CBCs in restoring normoglycaemia in both animals and humans. However, a direct effect of insulin and/or insulin-induced hypoglycaemia on CBC activity is not established in animal models. Therefore, the aim of this study was to evaluate in vivo effects of insulin and insulin-induced hypoglycaemia on CBC activity and cardiorespiration in a preclinical large animal model. The carotid sinus nerve (CSN) activity and cardiorespiratory responses to sodium cyanide (NaCN; 25 µg/kg) were compared before (euglycaemic) and after (hypoglycaemic) intracarotid administration of insulin (12.5–100 µU/dogs) in beagle dogs. Insulin administration increased CSN activity and minute ventilation ((Formula presented.) E) with significant (P < 0.0001) effects on heart rate and blood pressure. Insulin-mediated effects on CSN and cardiorespiration were sustained and the change in (Formula presented.) E was driven by tidal volume only. Insulin significantly (P < 0.0001) lowered blood glucose level. NaCN-mediated CSN activity and (Formula presented.) E were significantly (P < 0.0001) augmented during insulin-induced hypoglycaemia. The augmented (Formula presented.) E was primarily driven by respiratory frequency and partially by tidal volume. The cardiovascular reflex response mediated through CBC stimulation was significantly (P < 0.0001) exacerbated during insulin-induced hypoglycaemia. Collectively, these results demonstrate direct effects of insulin and insulin-induced hypoglycaemia on CBC chemosensitivity to potentiate CBC-mediated neuroregulatory pathways to initiate adaptive neuroendocrine and cardiorespiratory counter-regulatory responses to restore normoglycaemia.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNBaby, Santhosh M.Zaidi, FaisalHunsberger, Gerald E.Sokal, DavidGupta, IshaConde, Silvia V.Chew, DanielRall, KristenCoatney, Robert W.2022-12-13T22:18:15Z2023-02-012023-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/146187eng0958-0670PURE: 48290201https://doi.org/10.1113/EP090584info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:27:14Zoai:run.unl.pt:10362/146187Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:52:31.738305Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Acute effects of insulin and insulin-induced hypoglycaemia on carotid body chemoreceptor activity and cardiorespiratory responses in dogs
title Acute effects of insulin and insulin-induced hypoglycaemia on carotid body chemoreceptor activity and cardiorespiratory responses in dogs
spellingShingle Acute effects of insulin and insulin-induced hypoglycaemia on carotid body chemoreceptor activity and cardiorespiratory responses in dogs
Baby, Santhosh M.
carotid body
carotid body chemoreceptors
dogs
hypoglycaemia
insulin
sodium cyanide
Physiology
Nutrition and Dietetics
Physiology (medical)
title_short Acute effects of insulin and insulin-induced hypoglycaemia on carotid body chemoreceptor activity and cardiorespiratory responses in dogs
title_full Acute effects of insulin and insulin-induced hypoglycaemia on carotid body chemoreceptor activity and cardiorespiratory responses in dogs
title_fullStr Acute effects of insulin and insulin-induced hypoglycaemia on carotid body chemoreceptor activity and cardiorespiratory responses in dogs
title_full_unstemmed Acute effects of insulin and insulin-induced hypoglycaemia on carotid body chemoreceptor activity and cardiorespiratory responses in dogs
title_sort Acute effects of insulin and insulin-induced hypoglycaemia on carotid body chemoreceptor activity and cardiorespiratory responses in dogs
author Baby, Santhosh M.
author_facet Baby, Santhosh M.
Zaidi, Faisal
Hunsberger, Gerald E.
Sokal, David
Gupta, Isha
Conde, Silvia V.
Chew, Daniel
Rall, Kristen
Coatney, Robert W.
author_role author
author2 Zaidi, Faisal
Hunsberger, Gerald E.
Sokal, David
Gupta, Isha
Conde, Silvia V.
Chew, Daniel
Rall, Kristen
Coatney, Robert W.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Baby, Santhosh M.
Zaidi, Faisal
Hunsberger, Gerald E.
Sokal, David
Gupta, Isha
Conde, Silvia V.
Chew, Daniel
Rall, Kristen
Coatney, Robert W.
dc.subject.por.fl_str_mv carotid body
carotid body chemoreceptors
dogs
hypoglycaemia
insulin
sodium cyanide
Physiology
Nutrition and Dietetics
Physiology (medical)
topic carotid body
carotid body chemoreceptors
dogs
hypoglycaemia
insulin
sodium cyanide
Physiology
Nutrition and Dietetics
Physiology (medical)
description Funding: Silvia V. Conde declares that Galvani Bioelectronics provided funds to support their work associated with Type 2 diabetic project.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-13T22:18:15Z
2023-02-01
2023-02-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/146187
url http://hdl.handle.net/10362/146187
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0958-0670
PURE: 48290201
https://doi.org/10.1113/EP090584
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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