Detection of mcr-1 Gene in undefined Vibrio species isolated from clams

Detalhes bibliográficos
Autor(a) principal: Valdez, Christian
Data de Publicação: 2022
Outros Autores: Costa, Cátia, Simões, Marco, Carvalho, Carla C. C. R. De, Baptista, Teresa, Campos, Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.8/6723
Resumo: Funding: This study had the support of Fundação para a Ciência e a Tecnologia (FCT) through the Strategic Project UID/MAR/04292/2020 granted to MARE and grant agreement nº POCI‐01‐0247‐FEDER‐035234; AlgaValor, Portugal 2020 program.
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spelling Detection of mcr-1 Gene in undefined Vibrio species isolated from clamsAntimicrobial resistanceColistinmcr‐1Vibrio spp.Funding: This study had the support of Fundação para a Ciência e a Tecnologia (FCT) through the Strategic Project UID/MAR/04292/2020 granted to MARE and grant agreement nº POCI‐01‐0247‐FEDER‐035234; AlgaValor, Portugal 2020 program.The increase of antimicrobial resistant strains is leading to an emerging threat to public health. Pathogenic Vibrio are responsible for human and animal illness. The Enterobacteriaceae family includes microorganisms that affect humans, causing several infections. One of the main causes of human infection is related to the ingestion of undercooked seafood. Due to their filterfeeding habit, marine invertebrates, such as clams, are known to be a natural reservoir of specific microbial communities. In the present study, Vibrionaceae and coliforms microorganisms were isolated from clams. A microbial susceptibility test was performed using the disk diffusion method. From 43 presumptive Vibrio spp. and 17 coliforms, three Vibrio spp. with MICs to colistin > 512 mg L−1 were found. From the 23 antimicrobial resistance genes investigated, only the three isolates that showed phenotypic resistance to colistin contained the mcr‐1 gene. Genotypic analysis for virulence genes in EB07V indicated chiA gene presence. The results from the plasmid cure and transformation showed that the resistance is chromosomally mediated. Biochemical analysis and MLSA, on the basis of four protein‐coding gene sequences (recA, rpoB, groEL and dnaJ), grouped the isolates into the genus Vibrio but distinguished them as different from any known Vibrio spp.MDPIIC-OnlineValdez, ChristianCosta, CátiaSimões, MarcoCarvalho, Carla C. C. R. DeBaptista, TeresaCampos, Maria2022-02-28T14:59:42Z20222022-02-18T18:08:15Z2022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/6723engValdez, C.; Costa, C.; Simões, M.; de Carvalho, C.C.C.R.; Baptista, T.; Campos, M.J. Detection of mcr‐1 gene in undefined Vibrio species isolated from clams. Microorganisms 2022, 10, 394. https://doi.org/10.3390/ microorganisms100203942076-2607cv-prod-271982110.3390/microorganisms10020394info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:53:47Zoai:iconline.ipleiria.pt:10400.8/6723Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:49:53.535960Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Detection of mcr-1 Gene in undefined Vibrio species isolated from clams
title Detection of mcr-1 Gene in undefined Vibrio species isolated from clams
spellingShingle Detection of mcr-1 Gene in undefined Vibrio species isolated from clams
Valdez, Christian
Antimicrobial resistance
Colistin
mcr‐1
Vibrio spp.
title_short Detection of mcr-1 Gene in undefined Vibrio species isolated from clams
title_full Detection of mcr-1 Gene in undefined Vibrio species isolated from clams
title_fullStr Detection of mcr-1 Gene in undefined Vibrio species isolated from clams
title_full_unstemmed Detection of mcr-1 Gene in undefined Vibrio species isolated from clams
title_sort Detection of mcr-1 Gene in undefined Vibrio species isolated from clams
author Valdez, Christian
author_facet Valdez, Christian
Costa, Cátia
Simões, Marco
Carvalho, Carla C. C. R. De
Baptista, Teresa
Campos, Maria
author_role author
author2 Costa, Cátia
Simões, Marco
Carvalho, Carla C. C. R. De
Baptista, Teresa
Campos, Maria
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv IC-Online
dc.contributor.author.fl_str_mv Valdez, Christian
Costa, Cátia
Simões, Marco
Carvalho, Carla C. C. R. De
Baptista, Teresa
Campos, Maria
dc.subject.por.fl_str_mv Antimicrobial resistance
Colistin
mcr‐1
Vibrio spp.
topic Antimicrobial resistance
Colistin
mcr‐1
Vibrio spp.
description Funding: This study had the support of Fundação para a Ciência e a Tecnologia (FCT) through the Strategic Project UID/MAR/04292/2020 granted to MARE and grant agreement nº POCI‐01‐0247‐FEDER‐035234; AlgaValor, Portugal 2020 program.
publishDate 2022
dc.date.none.fl_str_mv 2022-02-28T14:59:42Z
2022
2022-02-18T18:08:15Z
2022-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.8/6723
url http://hdl.handle.net/10400.8/6723
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Valdez, C.; Costa, C.; Simões, M.; de Carvalho, C.C.C.R.; Baptista, T.; Campos, M.J. Detection of mcr‐1 gene in undefined Vibrio species isolated from clams. Microorganisms 2022, 10, 394. https://doi.org/10.3390/ microorganisms10020394
2076-2607
cv-prod-2719821
10.3390/microorganisms10020394
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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