Candida utilis metabolism and morphology under increased air pressure up to 12 bar
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/31899 |
Resumo: | Batch cultures of C. utilis CBS 621 were carried out in a pressurized reactor under increased air pressure up to 6 bar. The effect of total air pressure was also investigated in a high cell density fed-batch culture, raising the total air pressure from 1 bar to 12 bar. The results showed that the rise of air pressure, for both operation modes, led to a substantial enhancement of biomass production. Moreover, ethanol formation was significantly reduced at 6 bar and 12 bar air pressure, respectively for batch and fed-batch processes. A method using automatic image analysis for classification of C. utilis cells based on their morphology was developed and applied to experimental data. Morphological parameters such as single and budding cells, cell size and elongation factor, were analyzed to assess the pressure effect on yeast culture. No significant differences were observed in cell size distribution and yeast cells retained the typical oval form, even at 12 bar air pressure. In what biological aspects are concerned, it is possible to state that C. utilis CBS 621 can cope with hyperbaric stress, meaning that the use of increased air pressure is a suitable method for oxygenation enhancement of high density cultures of this strain. |
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Candida utilis metabolism and morphology under increased air pressure up to 12 barCandida utilisAir pressureFed-batch cultureMorphologyEthanol formationScience & TechnologyBatch cultures of C. utilis CBS 621 were carried out in a pressurized reactor under increased air pressure up to 6 bar. The effect of total air pressure was also investigated in a high cell density fed-batch culture, raising the total air pressure from 1 bar to 12 bar. The results showed that the rise of air pressure, for both operation modes, led to a substantial enhancement of biomass production. Moreover, ethanol formation was significantly reduced at 6 bar and 12 bar air pressure, respectively for batch and fed-batch processes. A method using automatic image analysis for classification of C. utilis cells based on their morphology was developed and applied to experimental data. Morphological parameters such as single and budding cells, cell size and elongation factor, were analyzed to assess the pressure effect on yeast culture. No significant differences were observed in cell size distribution and yeast cells retained the typical oval form, even at 12 bar air pressure. In what biological aspects are concerned, it is possible to state that C. utilis CBS 621 can cope with hyperbaric stress, meaning that the use of increased air pressure is a suitable method for oxygenation enhancement of high density cultures of this strain.The authors acknowledge the financial support provided by "Fundacao para a Ciencia e Tecnologia", Portugal.ElsevierUniversidade do MinhoPinheiro, RitaLopes, MarleneBelo, IsabelMota, M.20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31899eng1359-511310.1016/j.procbio.2013.11.014http://www.sciencedirect.com/science/journal/13595113/49/3info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:49:48Zoai:repositorium.sdum.uminho.pt:1822/31899Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:48:22.258113Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Candida utilis metabolism and morphology under increased air pressure up to 12 bar |
title |
Candida utilis metabolism and morphology under increased air pressure up to 12 bar |
spellingShingle |
Candida utilis metabolism and morphology under increased air pressure up to 12 bar Pinheiro, Rita Candida utilis Air pressure Fed-batch culture Morphology Ethanol formation Science & Technology |
title_short |
Candida utilis metabolism and morphology under increased air pressure up to 12 bar |
title_full |
Candida utilis metabolism and morphology under increased air pressure up to 12 bar |
title_fullStr |
Candida utilis metabolism and morphology under increased air pressure up to 12 bar |
title_full_unstemmed |
Candida utilis metabolism and morphology under increased air pressure up to 12 bar |
title_sort |
Candida utilis metabolism and morphology under increased air pressure up to 12 bar |
author |
Pinheiro, Rita |
author_facet |
Pinheiro, Rita Lopes, Marlene Belo, Isabel Mota, M. |
author_role |
author |
author2 |
Lopes, Marlene Belo, Isabel Mota, M. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Pinheiro, Rita Lopes, Marlene Belo, Isabel Mota, M. |
dc.subject.por.fl_str_mv |
Candida utilis Air pressure Fed-batch culture Morphology Ethanol formation Science & Technology |
topic |
Candida utilis Air pressure Fed-batch culture Morphology Ethanol formation Science & Technology |
description |
Batch cultures of C. utilis CBS 621 were carried out in a pressurized reactor under increased air pressure up to 6 bar. The effect of total air pressure was also investigated in a high cell density fed-batch culture, raising the total air pressure from 1 bar to 12 bar. The results showed that the rise of air pressure, for both operation modes, led to a substantial enhancement of biomass production. Moreover, ethanol formation was significantly reduced at 6 bar and 12 bar air pressure, respectively for batch and fed-batch processes. A method using automatic image analysis for classification of C. utilis cells based on their morphology was developed and applied to experimental data. Morphological parameters such as single and budding cells, cell size and elongation factor, were analyzed to assess the pressure effect on yeast culture. No significant differences were observed in cell size distribution and yeast cells retained the typical oval form, even at 12 bar air pressure. In what biological aspects are concerned, it is possible to state that C. utilis CBS 621 can cope with hyperbaric stress, meaning that the use of increased air pressure is a suitable method for oxygenation enhancement of high density cultures of this strain. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/31899 |
url |
http://hdl.handle.net/1822/31899 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1359-5113 10.1016/j.procbio.2013.11.014 http://www.sciencedirect.com/science/journal/13595113/49/3 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133061631180800 |