Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to sperm

Detalhes bibliográficos
Autor(a) principal: Jiang, Hua
Data de Publicação: 2015
Outros Autores: Yi, Jun, Boavida, Leonor C., Chen, Yuan, Becker, Jörg D., Köhler, Claudia, McCormick, Sheila
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.7/500
Resumo: An Arabidopsis pollen grain (male gametophyte) consists of three cells: the vegetative cell, which forms the pollen tube, and two sperm cells enclosed within the vegetative cell. It is still unclear if there is intercellular communication between the vegetative cell and the sperm cells. Here we show that ABA-hypersensitive germination3 (AHG3), encoding a protein phosphatase, is specifically transcribed in the vegetative cell but predominantly translated in sperm cells. We used a series of deletion constructs and promoter exchanges to document transport of AHG3 transcripts from the vegetative cell to sperm and showed that their transport requires sequences in both the 5' UTR and the coding region. Thus, in addition its known role in transporting sperm during pollen tube growth, the vegetative cell also contributes transcripts to the sperm cells.
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spelling Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to spermpollenspermmRNA transportprotein phosphatase 2Cvegetative nucleusAn Arabidopsis pollen grain (male gametophyte) consists of three cells: the vegetative cell, which forms the pollen tube, and two sperm cells enclosed within the vegetative cell. It is still unclear if there is intercellular communication between the vegetative cell and the sperm cells. Here we show that ABA-hypersensitive germination3 (AHG3), encoding a protein phosphatase, is specifically transcribed in the vegetative cell but predominantly translated in sperm cells. We used a series of deletion constructs and promoter exchanges to document transport of AHG3 transcripts from the vegetative cell to sperm and showed that their transport requires sequences in both the 5' UTR and the coding region. Thus, in addition its known role in transporting sperm during pollen tube growth, the vegetative cell also contributes transcripts to the sperm cells.US Department of Agriculture-Agricultural Research Service Current Research Information System Grant: (5335–21000–030–00D), European Research Council Starting Independent Researcher grant.National Academy of SciencesARCAJiang, HuaYi, JunBoavida, Leonor C.Chen, YuanBecker, Jörg D.Köhler, ClaudiaMcCormick, Sheila2015-11-18T15:21:14Z2015-10-272015-10-27T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/500engHua Jiang, Jun Yi, Leonor C. Boavida, Yuan Chen, Jörg D. Becker, Claudia Köhler, and Sheila McCormick Intercellular communication in Arabidopsis thaliana pollen discovered via AHG3 transcript movement from the vegetative cell to sperm PNAS 2015 112: 13378-13383.10.1073/pnas.1510854112info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:34:53Zoai:arca.igc.gulbenkian.pt:10400.7/500Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:46.327566Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to sperm
title Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to sperm
spellingShingle Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to sperm
Jiang, Hua
pollen
sperm
mRNA transport
protein phosphatase 2C
vegetative nucleus
title_short Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to sperm
title_full Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to sperm
title_fullStr Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to sperm
title_full_unstemmed Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to sperm
title_sort Intercellular communication inArabidopsis thalianapollen discovered viaAHG3transcript movement from the vegetative cell to sperm
author Jiang, Hua
author_facet Jiang, Hua
Yi, Jun
Boavida, Leonor C.
Chen, Yuan
Becker, Jörg D.
Köhler, Claudia
McCormick, Sheila
author_role author
author2 Yi, Jun
Boavida, Leonor C.
Chen, Yuan
Becker, Jörg D.
Köhler, Claudia
McCormick, Sheila
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv ARCA
dc.contributor.author.fl_str_mv Jiang, Hua
Yi, Jun
Boavida, Leonor C.
Chen, Yuan
Becker, Jörg D.
Köhler, Claudia
McCormick, Sheila
dc.subject.por.fl_str_mv pollen
sperm
mRNA transport
protein phosphatase 2C
vegetative nucleus
topic pollen
sperm
mRNA transport
protein phosphatase 2C
vegetative nucleus
description An Arabidopsis pollen grain (male gametophyte) consists of three cells: the vegetative cell, which forms the pollen tube, and two sperm cells enclosed within the vegetative cell. It is still unclear if there is intercellular communication between the vegetative cell and the sperm cells. Here we show that ABA-hypersensitive germination3 (AHG3), encoding a protein phosphatase, is specifically transcribed in the vegetative cell but predominantly translated in sperm cells. We used a series of deletion constructs and promoter exchanges to document transport of AHG3 transcripts from the vegetative cell to sperm and showed that their transport requires sequences in both the 5' UTR and the coding region. Thus, in addition its known role in transporting sperm during pollen tube growth, the vegetative cell also contributes transcripts to the sperm cells.
publishDate 2015
dc.date.none.fl_str_mv 2015-11-18T15:21:14Z
2015-10-27
2015-10-27T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.7/500
url http://hdl.handle.net/10400.7/500
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Hua Jiang, Jun Yi, Leonor C. Boavida, Yuan Chen, Jörg D. Becker, Claudia Köhler, and Sheila McCormick Intercellular communication in Arabidopsis thaliana pollen discovered via AHG3 transcript movement from the vegetative cell to sperm PNAS 2015 112: 13378-13383.
10.1073/pnas.1510854112
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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