Diversity of bacteria in the marine sponge Aplysina fulva in Brazilian coastal waters
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/11732 |
Resumo: | Microorganisms can account for up to 60% of the fresh weight of marine sponges. Marine sponges have been hypothesized to serve as accumulation spots of particular microbial communities, but it is unknown to what extent these communities are directed by the organism or the site or occur randomly. To address this question, we assessed the composition of specific bacterial communities associated with Aplysina fulva, one of the prevalent sponge species inhabiting Brazilian waters. Specimens of A. fulva and surrounding seawater were collected in triplicate in shallow water at two sites, Caboclo Island and Tartaruga beach, Buzios, Brazil. Total community DNA was extracted from the samples using "direct" and "indirect" approaches. 16S rRNA-based PCR-denaturing gradient gel electrophoresis (PCR-DGGE) analyses of the total bacterial community and of specific bacterial groups-Pseudomonas and Actinobacteria-revealed that the structure of these assemblages in A. fulva differed drastically from that observed in seawater. The DNA extraction methodology and sampling site were determinative for the composition of actinobacterial communities in A. fulva. However, no such effects could be gleaned from total bacterial and Pseudomonas PCR-DGGE profiles. Bacterial 16S rRNA gene clone libraries constructed from directly and indirectly extracted DNA did not differ significantly with respect to diversity and composition. Altogether, the libraries encompassed 15 bacterial phyla and the candidate division TM7. Clone sequences affiliated with the Cyanobacteria, Chloroflexi, Gamma-and Alphaproteobacteria, Actinobacteria, Bacteroidetes, and Acidobacteria were, in this order, most abundant. The bacterial communities associated with the A. fulva specimens were distinct and differed from those described in studies of sponge-associated microbiota performed with other sponge species. |
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Diversity of bacteria in the marine sponge Aplysina fulva in Brazilian coastal waters16S Ribosomal-RnaPolyketide synthase genesPseudoceratina-ClavataMicrobial communityAntimicrobial activitiesCyanobacterial SymbiontMediterranean spongesPhylogenetic analysisAnaerobic-bacteriaSoilMicroorganisms can account for up to 60% of the fresh weight of marine sponges. Marine sponges have been hypothesized to serve as accumulation spots of particular microbial communities, but it is unknown to what extent these communities are directed by the organism or the site or occur randomly. To address this question, we assessed the composition of specific bacterial communities associated with Aplysina fulva, one of the prevalent sponge species inhabiting Brazilian waters. Specimens of A. fulva and surrounding seawater were collected in triplicate in shallow water at two sites, Caboclo Island and Tartaruga beach, Buzios, Brazil. Total community DNA was extracted from the samples using "direct" and "indirect" approaches. 16S rRNA-based PCR-denaturing gradient gel electrophoresis (PCR-DGGE) analyses of the total bacterial community and of specific bacterial groups-Pseudomonas and Actinobacteria-revealed that the structure of these assemblages in A. fulva differed drastically from that observed in seawater. The DNA extraction methodology and sampling site were determinative for the composition of actinobacterial communities in A. fulva. However, no such effects could be gleaned from total bacterial and Pseudomonas PCR-DGGE profiles. Bacterial 16S rRNA gene clone libraries constructed from directly and indirectly extracted DNA did not differ significantly with respect to diversity and composition. Altogether, the libraries encompassed 15 bacterial phyla and the candidate division TM7. Clone sequences affiliated with the Cyanobacteria, Chloroflexi, Gamma-and Alphaproteobacteria, Actinobacteria, Bacteroidetes, and Acidobacteria were, in this order, most abundant. The bacterial communities associated with the A. fulva specimens were distinct and differed from those described in studies of sponge-associated microbiota performed with other sponge species.Soil Biotechnology Foundation, Wageningen, The Netherlands; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico; Fundacao de Amparo a Pesquisa do Estado do Rio de JaneiroAmerican Society for MicrobiologySapientiaHardoim, C. C. P.Costa, R.Araujo, F. V.Hajdu, E.Peixoto, R.Lins, U.Rosado, A. S.van Elsas, J. D.2018-12-07T14:57:51Z2009-052009-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/11732eng0099-224010.1128/AEM.02101-08info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:23:34Zoai:sapientia.ualg.pt:10400.1/11732Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:03:11.766601Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Diversity of bacteria in the marine sponge Aplysina fulva in Brazilian coastal waters |
title |
Diversity of bacteria in the marine sponge Aplysina fulva in Brazilian coastal waters |
spellingShingle |
Diversity of bacteria in the marine sponge Aplysina fulva in Brazilian coastal waters Hardoim, C. C. P. 16S Ribosomal-Rna Polyketide synthase genes Pseudoceratina-Clavata Microbial community Antimicrobial activities Cyanobacterial Symbiont Mediterranean sponges Phylogenetic analysis Anaerobic-bacteria Soil |
title_short |
Diversity of bacteria in the marine sponge Aplysina fulva in Brazilian coastal waters |
title_full |
Diversity of bacteria in the marine sponge Aplysina fulva in Brazilian coastal waters |
title_fullStr |
Diversity of bacteria in the marine sponge Aplysina fulva in Brazilian coastal waters |
title_full_unstemmed |
Diversity of bacteria in the marine sponge Aplysina fulva in Brazilian coastal waters |
title_sort |
Diversity of bacteria in the marine sponge Aplysina fulva in Brazilian coastal waters |
author |
Hardoim, C. C. P. |
author_facet |
Hardoim, C. C. P. Costa, R. Araujo, F. V. Hajdu, E. Peixoto, R. Lins, U. Rosado, A. S. van Elsas, J. D. |
author_role |
author |
author2 |
Costa, R. Araujo, F. V. Hajdu, E. Peixoto, R. Lins, U. Rosado, A. S. van Elsas, J. D. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Hardoim, C. C. P. Costa, R. Araujo, F. V. Hajdu, E. Peixoto, R. Lins, U. Rosado, A. S. van Elsas, J. D. |
dc.subject.por.fl_str_mv |
16S Ribosomal-Rna Polyketide synthase genes Pseudoceratina-Clavata Microbial community Antimicrobial activities Cyanobacterial Symbiont Mediterranean sponges Phylogenetic analysis Anaerobic-bacteria Soil |
topic |
16S Ribosomal-Rna Polyketide synthase genes Pseudoceratina-Clavata Microbial community Antimicrobial activities Cyanobacterial Symbiont Mediterranean sponges Phylogenetic analysis Anaerobic-bacteria Soil |
description |
Microorganisms can account for up to 60% of the fresh weight of marine sponges. Marine sponges have been hypothesized to serve as accumulation spots of particular microbial communities, but it is unknown to what extent these communities are directed by the organism or the site or occur randomly. To address this question, we assessed the composition of specific bacterial communities associated with Aplysina fulva, one of the prevalent sponge species inhabiting Brazilian waters. Specimens of A. fulva and surrounding seawater were collected in triplicate in shallow water at two sites, Caboclo Island and Tartaruga beach, Buzios, Brazil. Total community DNA was extracted from the samples using "direct" and "indirect" approaches. 16S rRNA-based PCR-denaturing gradient gel electrophoresis (PCR-DGGE) analyses of the total bacterial community and of specific bacterial groups-Pseudomonas and Actinobacteria-revealed that the structure of these assemblages in A. fulva differed drastically from that observed in seawater. The DNA extraction methodology and sampling site were determinative for the composition of actinobacterial communities in A. fulva. However, no such effects could be gleaned from total bacterial and Pseudomonas PCR-DGGE profiles. Bacterial 16S rRNA gene clone libraries constructed from directly and indirectly extracted DNA did not differ significantly with respect to diversity and composition. Altogether, the libraries encompassed 15 bacterial phyla and the candidate division TM7. Clone sequences affiliated with the Cyanobacteria, Chloroflexi, Gamma-and Alphaproteobacteria, Actinobacteria, Bacteroidetes, and Acidobacteria were, in this order, most abundant. The bacterial communities associated with the A. fulva specimens were distinct and differed from those described in studies of sponge-associated microbiota performed with other sponge species. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-05 2009-05-01T00:00:00Z 2018-12-07T14:57:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/11732 |
url |
http://hdl.handle.net/10400.1/11732 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0099-2240 10.1128/AEM.02101-08 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Society for Microbiology |
publisher.none.fl_str_mv |
American Society for Microbiology |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133266178998272 |