Dilution and magnification effects on image analysis applications in activated sludge characterization

Detalhes bibliográficos
Autor(a) principal: Mesquita, D.P.
Data de Publicação: 2010
Outros Autores: Dias, O., Elias, R.A.V., Amaral, António L., Ferreira, E.C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/17685
Resumo: The properties of activated sludge systems can be characterized using image analysis procedures. When these systems operate with high biomass content, accurate sludge characterization requires samples to be diluted. Selection of the best image acquisition magnification is directly related to the amount of biomass screened. The aim of the present study was to survey the effects of dilution and magnification on the assessment of aggregated and filamentous bacterial content and structure using image analysis procedures. Assessments of biomass content and structure were affected by dilutions. Therefore, the correct operating dilution requires careful consideration. Moreover, the acquisition methodology comprising a 100× magnification allowed data on aggregated and filamentous biomass to be determined and smaller aggregates to be identified and characterized, without affecting the accuracy of lower magnifications regarding biomass representativeness.
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spelling Dilution and magnification effects on image analysis applications in activated sludge characterizationActivated sludgeImage analysisDilutionMagnificationAggregatesFilamentsThe properties of activated sludge systems can be characterized using image analysis procedures. When these systems operate with high biomass content, accurate sludge characterization requires samples to be diluted. Selection of the best image acquisition magnification is directly related to the amount of biomass screened. The aim of the present study was to survey the effects of dilution and magnification on the assessment of aggregated and filamentous bacterial content and structure using image analysis procedures. Assessments of biomass content and structure were affected by dilutions. Therefore, the correct operating dilution requires careful consideration. Moreover, the acquisition methodology comprising a 100× magnification allowed data on aggregated and filamentous biomass to be determined and smaller aggregates to be identified and characterized, without affecting the accuracy of lower magnifications regarding biomass representativeness.The authors acknowledge financial support to D.P.M. and O.D. through the grant SFRH/BD/32329/2006 and the project POCI/AMB/57069/2004, respectively, provided by Fundação para a Ciência e Tecnologia ~Portugal!. The authors also express their gratitude to AGERE ~Empresa de Águas, Efluentes e Resíduos de Braga – EM! and AGS ~Administração e Gestão de Sistemas de Salubridade, S.A.! for their cooperation.Biblioteca Digital do IPBMesquita, D.P.Dias, O.Elias, R.A.V.Amaral, António L.Ferreira, E.C.2018-06-08T14:39:10Z20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/17685engMesquita, D.P.; Dias, O.; Elias, R.A.V.; Amaral, A.L.; Ferreira, E.C. (2010). Dilution and magnification effects on image analysis applications in activated sludge characterization. Microscopy and Microanalysis. ISSN 1431-9276. 16:5, p. 561-5681431-927610.1017/S1431927610093785info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:40:31Zoai:bibliotecadigital.ipb.pt:10198/17685Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:07:30.510792Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Dilution and magnification effects on image analysis applications in activated sludge characterization
title Dilution and magnification effects on image analysis applications in activated sludge characterization
spellingShingle Dilution and magnification effects on image analysis applications in activated sludge characterization
Mesquita, D.P.
Activated sludge
Image analysis
Dilution
Magnification
Aggregates
Filaments
title_short Dilution and magnification effects on image analysis applications in activated sludge characterization
title_full Dilution and magnification effects on image analysis applications in activated sludge characterization
title_fullStr Dilution and magnification effects on image analysis applications in activated sludge characterization
title_full_unstemmed Dilution and magnification effects on image analysis applications in activated sludge characterization
title_sort Dilution and magnification effects on image analysis applications in activated sludge characterization
author Mesquita, D.P.
author_facet Mesquita, D.P.
Dias, O.
Elias, R.A.V.
Amaral, António L.
Ferreira, E.C.
author_role author
author2 Dias, O.
Elias, R.A.V.
Amaral, António L.
Ferreira, E.C.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Mesquita, D.P.
Dias, O.
Elias, R.A.V.
Amaral, António L.
Ferreira, E.C.
dc.subject.por.fl_str_mv Activated sludge
Image analysis
Dilution
Magnification
Aggregates
Filaments
topic Activated sludge
Image analysis
Dilution
Magnification
Aggregates
Filaments
description The properties of activated sludge systems can be characterized using image analysis procedures. When these systems operate with high biomass content, accurate sludge characterization requires samples to be diluted. Selection of the best image acquisition magnification is directly related to the amount of biomass screened. The aim of the present study was to survey the effects of dilution and magnification on the assessment of aggregated and filamentous bacterial content and structure using image analysis procedures. Assessments of biomass content and structure were affected by dilutions. Therefore, the correct operating dilution requires careful consideration. Moreover, the acquisition methodology comprising a 100× magnification allowed data on aggregated and filamentous biomass to be determined and smaller aggregates to be identified and characterized, without affecting the accuracy of lower magnifications regarding biomass representativeness.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01T00:00:00Z
2018-06-08T14:39:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/17685
url http://hdl.handle.net/10198/17685
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Mesquita, D.P.; Dias, O.; Elias, R.A.V.; Amaral, A.L.; Ferreira, E.C. (2010). Dilution and magnification effects on image analysis applications in activated sludge characterization. Microscopy and Microanalysis. ISSN 1431-9276. 16:5, p. 561-568
1431-9276
10.1017/S1431927610093785
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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