A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks

Detalhes bibliográficos
Autor(a) principal: Simões, Lúcia Chaves
Data de Publicação: 2023
Outros Autores: Oliveira, Isabel, Borges, Anabela, Gomes, Inês Bezerra, Simões, Manuel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/88381
Resumo: The following material is available online at: https://journals.asm.org/doi/10.1128/jmbe.00099-23
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spelling A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasksagitationhydrodynamic forcemicrobial growthshake flasksstandardizationThe following material is available online at: https://journals.asm.org/doi/10.1128/jmbe.00099-23Shake flask cultivation is a routine technique in microbiology and biotechnology laboratories where cell growth can be affected by the hydrodynamic conditions, which depend on the agitation velocity, shaking diameter, and shake flask size. Liquid agitation is implemented inherently to increase aeration, substrate transfer to the cells, and prevent sedimentation, disregarding the role of hydrodynamics in microbial growth and metabolism. Here, we present a simple approach to help standardize the hydrodynamic forces in orbital shakers to increase the experimental accuracy and reproducibility and give students a better knowledge of the significance of the agitation process in microbial growth.This research was funded by LA/P/0045/2020 (ALiCE), UIDB/00511/2020, and UIDP/00511/2020 (LEPABE), funded by national funds through FCT/MCTES (PIDDAC); UIDB/04469/2020 (CEB) and LABBELS—Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020; Germirrad-POCI-01–0247-FEDER-072237, funded by FEDER funds through COMPETE2020–Programa Operacional Competitividade e Internacionalização (POCI) and by national funds (PIDDAC) through FCT/MCTES.info:eu-repo/semantics/publishedVersionAmerican Society for MicrobiologyUniversidade do MinhoSimões, Lúcia ChavesOliveira, IsabelBorges, AnabelaGomes, Inês BezerraSimões, Manuel2023-122023-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/88381engSimões, L. C., Oliveira, I., Borges, A., Gomes, I. B., & Simões, M. (2023, December 14). A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks. (L. J. MacDonald, Ed.), Journal of Microbiology & Biology Education. American Society for Microbiology. http://doi.org/10.1128/jmbe.00099-231935-78771935-788510.1128/jmbe.00099-23https://journals.asm.org/doi/10.1128/jmbe.00099-23info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-03T01:20:45Zoai:repositorium.sdum.uminho.pt:1822/88381Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:07:31.130991Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks
title A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks
spellingShingle A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks
Simões, Lúcia Chaves
agitation
hydrodynamic force
microbial growth
shake flasks
standardization
title_short A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks
title_full A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks
title_fullStr A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks
title_full_unstemmed A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks
title_sort A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks
author Simões, Lúcia Chaves
author_facet Simões, Lúcia Chaves
Oliveira, Isabel
Borges, Anabela
Gomes, Inês Bezerra
Simões, Manuel
author_role author
author2 Oliveira, Isabel
Borges, Anabela
Gomes, Inês Bezerra
Simões, Manuel
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Simões, Lúcia Chaves
Oliveira, Isabel
Borges, Anabela
Gomes, Inês Bezerra
Simões, Manuel
dc.subject.por.fl_str_mv agitation
hydrodynamic force
microbial growth
shake flasks
standardization
topic agitation
hydrodynamic force
microbial growth
shake flasks
standardization
description The following material is available online at: https://journals.asm.org/doi/10.1128/jmbe.00099-23
publishDate 2023
dc.date.none.fl_str_mv 2023-12
2023-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/88381
url https://hdl.handle.net/1822/88381
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Simões, L. C., Oliveira, I., Borges, A., Gomes, I. B., & Simões, M. (2023, December 14). A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks. (L. J. MacDonald, Ed.), Journal of Microbiology & Biology Education. American Society for Microbiology. http://doi.org/10.1128/jmbe.00099-23
1935-7877
1935-7885
10.1128/jmbe.00099-23
https://journals.asm.org/doi/10.1128/jmbe.00099-23
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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