Preliminary structural characterization of human SOUL, a haem-binding protein

Detalhes bibliográficos
Autor(a) principal: Freire, Filipe
Data de Publicação: 2009
Outros Autores: Romão, Maria João, MacEdo, Anjos L., Aveiro, Susana S., Goodfellow, Brian J., Carvalho, Ana Luísa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1107/S174430910902291X
Resumo: The authors acknowledge financial support from Fundacao para a Ciencia e Tecnologia, Portugal through grant SFRH/BPD/30239/2006 and project PTDC/QUI/64203/2006. The authors also acknowledge the ESRF, Grenoble (beamlines ID14- EH2, ID14- EH4, ID23- EH1 and ID-29) for access and technical support during data collection.
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spelling Preliminary structural characterization of human SOUL, a haem-binding proteinHaem-binding proteinsSOULBiophysicsStructural BiologyBiochemistryGeneticsCondensed Matter PhysicsThe authors acknowledge financial support from Fundacao para a Ciencia e Tecnologia, Portugal through grant SFRH/BPD/30239/2006 and project PTDC/QUI/64203/2006. The authors also acknowledge the ESRF, Grenoble (beamlines ID14- EH2, ID14- EH4, ID23- EH1 and ID-29) for access and technical support during data collection.Human SOUL (hSOUL) is a 23 kDa haem-binding protein that was first identified as the PP23 protein isolated from human full-term placentas. Here, the overexpression, purification and crystallization of hSOUL are reported. The crystals belonged to space group P6422, with unit-cell parameters a = b = 145, c = 60 Å and one protein molecule in the asymmetric unit. X-ray diffraction data were collected to 3.5 Å resolution at the ESRF. A preliminary model of the three-dimensional structure of hSOUL was obtained by molecular replacement using the structures of murine p22HBP (PDB codes 2gov and 2hva), obtained by solution NMR, as search models.DQ - Departamento de QuímicaCQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)RUNFreire, FilipeRomão, Maria JoãoMacEdo, Anjos L.Aveiro, Susana S.Goodfellow, Brian J.Carvalho, Ana Luísa2019-03-11T23:15:21Z2009-01-012009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4application/pdfhttps://doi.org/10.1107/S174430910902291Xeng1744-3091PURE: 12011100http://www.scopus.com/inward/record.url?scp=68149089746&partnerID=8YFLogxKhttps://doi.org/10.1107/S174430910902291Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:29:46Zoai:run.unl.pt:10362/62996Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:33:50.061061Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Preliminary structural characterization of human SOUL, a haem-binding protein
title Preliminary structural characterization of human SOUL, a haem-binding protein
spellingShingle Preliminary structural characterization of human SOUL, a haem-binding protein
Freire, Filipe
Haem-binding proteins
SOUL
Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics
title_short Preliminary structural characterization of human SOUL, a haem-binding protein
title_full Preliminary structural characterization of human SOUL, a haem-binding protein
title_fullStr Preliminary structural characterization of human SOUL, a haem-binding protein
title_full_unstemmed Preliminary structural characterization of human SOUL, a haem-binding protein
title_sort Preliminary structural characterization of human SOUL, a haem-binding protein
author Freire, Filipe
author_facet Freire, Filipe
Romão, Maria João
MacEdo, Anjos L.
Aveiro, Susana S.
Goodfellow, Brian J.
Carvalho, Ana Luísa
author_role author
author2 Romão, Maria João
MacEdo, Anjos L.
Aveiro, Susana S.
Goodfellow, Brian J.
Carvalho, Ana Luísa
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
CQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)
RUN
dc.contributor.author.fl_str_mv Freire, Filipe
Romão, Maria João
MacEdo, Anjos L.
Aveiro, Susana S.
Goodfellow, Brian J.
Carvalho, Ana Luísa
dc.subject.por.fl_str_mv Haem-binding proteins
SOUL
Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics
topic Haem-binding proteins
SOUL
Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics
description The authors acknowledge financial support from Fundacao para a Ciencia e Tecnologia, Portugal through grant SFRH/BPD/30239/2006 and project PTDC/QUI/64203/2006. The authors also acknowledge the ESRF, Grenoble (beamlines ID14- EH2, ID14- EH4, ID23- EH1 and ID-29) for access and technical support during data collection.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
2009-01-01T00:00:00Z
2019-03-11T23:15:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1107/S174430910902291X
url https://doi.org/10.1107/S174430910902291X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1744-3091
PURE: 12011100
http://www.scopus.com/inward/record.url?scp=68149089746&partnerID=8YFLogxK
https://doi.org/10.1107/S174430910902291X
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instacron:RCAAP
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