Preliminary structural characterization of human SOUL, a haem-binding protein

Detalhes bibliográficos
Autor(a) principal: Freire, Filipe
Data de Publicação: 2009
Outros Autores: Romão, Maria João, MacEdo, Anjos L., Aveiro, Susana S., Goodfellow, Brian J., Carvalho, Ana Luísa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
DOI: 10.1107/S174430910902291X
Texto Completo: https://doi.org/10.1107/S174430910902291X
Resumo: The authors acknowledge financial support from Fundacao para a Ciencia e Tecnologia, Portugal through grant SFRH/BPD/30239/2006 and project PTDC/QUI/64203/2006. The authors also acknowledge the ESRF, Grenoble (beamlines ID14- EH2, ID14- EH4, ID23- EH1 and ID-29) for access and technical support during data collection.
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spelling Preliminary structural characterization of human SOUL, a haem-binding proteinHaem-binding proteinsSOULBiophysicsStructural BiologyBiochemistryGeneticsCondensed Matter PhysicsThe authors acknowledge financial support from Fundacao para a Ciencia e Tecnologia, Portugal through grant SFRH/BPD/30239/2006 and project PTDC/QUI/64203/2006. The authors also acknowledge the ESRF, Grenoble (beamlines ID14- EH2, ID14- EH4, ID23- EH1 and ID-29) for access and technical support during data collection.Human SOUL (hSOUL) is a 23 kDa haem-binding protein that was first identified as the PP23 protein isolated from human full-term placentas. Here, the overexpression, purification and crystallization of hSOUL are reported. The crystals belonged to space group P6422, with unit-cell parameters a = b = 145, c = 60 Å and one protein molecule in the asymmetric unit. X-ray diffraction data were collected to 3.5 Å resolution at the ESRF. A preliminary model of the three-dimensional structure of hSOUL was obtained by molecular replacement using the structures of murine p22HBP (PDB codes 2gov and 2hva), obtained by solution NMR, as search models.DQ - Departamento de QuímicaCQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)RUNFreire, FilipeRomão, Maria JoãoMacEdo, Anjos L.Aveiro, Susana S.Goodfellow, Brian J.Carvalho, Ana Luísa2019-03-11T23:15:21Z2009-01-012009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4application/pdfhttps://doi.org/10.1107/S174430910902291Xeng1744-3091PURE: 12011100http://www.scopus.com/inward/record.url?scp=68149089746&partnerID=8YFLogxKhttps://doi.org/10.1107/S174430910902291Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:37:40Zoai:run.unl.pt:10362/62996Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:37:40Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Preliminary structural characterization of human SOUL, a haem-binding protein
title Preliminary structural characterization of human SOUL, a haem-binding protein
spellingShingle Preliminary structural characterization of human SOUL, a haem-binding protein
Preliminary structural characterization of human SOUL, a haem-binding protein
Freire, Filipe
Haem-binding proteins
SOUL
Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics
Freire, Filipe
Haem-binding proteins
SOUL
Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics
title_short Preliminary structural characterization of human SOUL, a haem-binding protein
title_full Preliminary structural characterization of human SOUL, a haem-binding protein
title_fullStr Preliminary structural characterization of human SOUL, a haem-binding protein
Preliminary structural characterization of human SOUL, a haem-binding protein
title_full_unstemmed Preliminary structural characterization of human SOUL, a haem-binding protein
Preliminary structural characterization of human SOUL, a haem-binding protein
title_sort Preliminary structural characterization of human SOUL, a haem-binding protein
author Freire, Filipe
author_facet Freire, Filipe
Freire, Filipe
Romão, Maria João
MacEdo, Anjos L.
Aveiro, Susana S.
Goodfellow, Brian J.
Carvalho, Ana Luísa
Romão, Maria João
MacEdo, Anjos L.
Aveiro, Susana S.
Goodfellow, Brian J.
Carvalho, Ana Luísa
author_role author
author2 Romão, Maria João
MacEdo, Anjos L.
Aveiro, Susana S.
Goodfellow, Brian J.
Carvalho, Ana Luísa
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
CQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)
RUN
dc.contributor.author.fl_str_mv Freire, Filipe
Romão, Maria João
MacEdo, Anjos L.
Aveiro, Susana S.
Goodfellow, Brian J.
Carvalho, Ana Luísa
dc.subject.por.fl_str_mv Haem-binding proteins
SOUL
Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics
topic Haem-binding proteins
SOUL
Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics
description The authors acknowledge financial support from Fundacao para a Ciencia e Tecnologia, Portugal through grant SFRH/BPD/30239/2006 and project PTDC/QUI/64203/2006. The authors also acknowledge the ESRF, Grenoble (beamlines ID14- EH2, ID14- EH4, ID23- EH1 and ID-29) for access and technical support during data collection.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
2009-01-01T00:00:00Z
2019-03-11T23:15:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1107/S174430910902291X
url https://doi.org/10.1107/S174430910902291X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1744-3091
PURE: 12011100
http://www.scopus.com/inward/record.url?scp=68149089746&partnerID=8YFLogxK
https://doi.org/10.1107/S174430910902291X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 4
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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dc.identifier.doi.none.fl_str_mv 10.1107/S174430910902291X

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