Diagnosis of Helicobacter pylori infection
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/59284 |
Resumo: | Several studies addressed the usefulness of using molecular techniques for the diagnosis of Helicobacter pylori, pointing to the emerging paradigm change for clinical microbiology laboratories. During the last year, new variations of PCR-based approaches (formats, probes), new targets, as well as non-PCR based methods were described. In most cases, molecular methods combine detection of H. pylori with antimicrobial susceptibility testing, to predict antibiotic treatment outcome and guide clinicians in their choice of therapy. Macrolides and fluoroquinolones, for which resistance determinants are well known and are a major cause of treatment failure, remain the main targets of the classical molecular methods. Several other non-molecular methods, based on different technologies, were described for direct or indirect detection of H. pylori in different biological samples. Newly described endoscopic technologies offer advantages in the identification of patients at high risk of developing gastric cancer. Regarding serology, the importance of using optimised serological assays in specific geographical regions, employing local H. pylori strains for preparing antigens was highlighted. Next Generation Sequencing (NGS) based methods appear as a powerful tool to predict antibiotic resistance and will soon lead to a paradigm shift in the transition from culture-based to molecular-based susceptibility testing. In this context, due to the complexity of the resistance mechanisms and to allow the inclusion of further antibiotics, more comprehensive genotypic-phenotypic comparisons are needed. Additionally, the review of papers on H. pylori diagnosis published last year provided information concerning current prevalence of infection and antibiotic resistance in distinct geographies, indicating a tendency towards declining prevalence and rising antibiotic resistance. |
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Diagnosis of Helicobacter pylori infectionSeveral studies addressed the usefulness of using molecular techniques for the diagnosis of Helicobacter pylori, pointing to the emerging paradigm change for clinical microbiology laboratories. During the last year, new variations of PCR-based approaches (formats, probes), new targets, as well as non-PCR based methods were described. In most cases, molecular methods combine detection of H. pylori with antimicrobial susceptibility testing, to predict antibiotic treatment outcome and guide clinicians in their choice of therapy. Macrolides and fluoroquinolones, for which resistance determinants are well known and are a major cause of treatment failure, remain the main targets of the classical molecular methods. Several other non-molecular methods, based on different technologies, were described for direct or indirect detection of H. pylori in different biological samples. Newly described endoscopic technologies offer advantages in the identification of patients at high risk of developing gastric cancer. Regarding serology, the importance of using optimised serological assays in specific geographical regions, employing local H. pylori strains for preparing antigens was highlighted. Next Generation Sequencing (NGS) based methods appear as a powerful tool to predict antibiotic resistance and will soon lead to a paradigm shift in the transition from culture-based to molecular-based susceptibility testing. In this context, due to the complexity of the resistance mechanisms and to allow the inclusion of further antibiotics, more comprehensive genotypic-phenotypic comparisons are needed. Additionally, the review of papers on H. pylori diagnosis published last year provided information concerning current prevalence of infection and antibiotic resistance in distinct geographies, indicating a tendency towards declining prevalence and rising antibiotic resistance.F.F.V. is funded by Fundação para a Ciência e a Tecnologia through an Assistant Researcher grant CEECIND/03023/2017, and project grants (PTDC/BTM-SAL/28978/2017 and PTDC/BTM-TEC/3238/2020).Verduci Editore / European Helicobacter and Microbiota Study Group – EHMSGRepositório da Universidade de LisboaVale, Filipa F.Oleastro, Mónica2023-09-14T10:26:39Z20222023-03-07T14:57:01Z2022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/59284engVale FF, Oleastro M. Review: Diagnosis of Helicobacter pylori infection. Microbiota in Health and Disease [Internet]. setembro de 2022;4(3). Disponível em: https://doi.org/10.26355/mhd_20229_725cv-prod-315872910.26355/mhd_20229_725info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:04:15Zoai:repositorio.ul.pt:10451/59284Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:07:06.671509Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Diagnosis of Helicobacter pylori infection |
title |
Diagnosis of Helicobacter pylori infection |
spellingShingle |
Diagnosis of Helicobacter pylori infection Vale, Filipa F. |
title_short |
Diagnosis of Helicobacter pylori infection |
title_full |
Diagnosis of Helicobacter pylori infection |
title_fullStr |
Diagnosis of Helicobacter pylori infection |
title_full_unstemmed |
Diagnosis of Helicobacter pylori infection |
title_sort |
Diagnosis of Helicobacter pylori infection |
author |
Vale, Filipa F. |
author_facet |
Vale, Filipa F. Oleastro, Mónica |
author_role |
author |
author2 |
Oleastro, Mónica |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Vale, Filipa F. Oleastro, Mónica |
description |
Several studies addressed the usefulness of using molecular techniques for the diagnosis of Helicobacter pylori, pointing to the emerging paradigm change for clinical microbiology laboratories. During the last year, new variations of PCR-based approaches (formats, probes), new targets, as well as non-PCR based methods were described. In most cases, molecular methods combine detection of H. pylori with antimicrobial susceptibility testing, to predict antibiotic treatment outcome and guide clinicians in their choice of therapy. Macrolides and fluoroquinolones, for which resistance determinants are well known and are a major cause of treatment failure, remain the main targets of the classical molecular methods. Several other non-molecular methods, based on different technologies, were described for direct or indirect detection of H. pylori in different biological samples. Newly described endoscopic technologies offer advantages in the identification of patients at high risk of developing gastric cancer. Regarding serology, the importance of using optimised serological assays in specific geographical regions, employing local H. pylori strains for preparing antigens was highlighted. Next Generation Sequencing (NGS) based methods appear as a powerful tool to predict antibiotic resistance and will soon lead to a paradigm shift in the transition from culture-based to molecular-based susceptibility testing. In this context, due to the complexity of the resistance mechanisms and to allow the inclusion of further antibiotics, more comprehensive genotypic-phenotypic comparisons are needed. Additionally, the review of papers on H. pylori diagnosis published last year provided information concerning current prevalence of infection and antibiotic resistance in distinct geographies, indicating a tendency towards declining prevalence and rising antibiotic resistance. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 2022-01-01T00:00:00Z 2023-09-14T10:26:39Z 2023-03-07T14:57:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/59284 |
url |
http://hdl.handle.net/10451/59284 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Vale FF, Oleastro M. Review: Diagnosis of Helicobacter pylori infection. Microbiota in Health and Disease [Internet]. setembro de 2022;4(3). Disponível em: https://doi.org/10.26355/mhd_20229_725 cv-prod-3158729 10.26355/mhd_20229_725 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Verduci Editore / European Helicobacter and Microbiota Study Group – EHMSG |
publisher.none.fl_str_mv |
Verduci Editore / European Helicobacter and Microbiota Study Group – EHMSG |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799134624648003584 |