Shielding of actin by the endoplasmic reticulum impacts nuclear positioning
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/53158 |
Resumo: | © The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
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Shielding of actin by the endoplasmic reticulum impacts nuclear positioning© The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.Nuclear position is central to cell polarization, and its disruption is associated with various pathologies. The nucleus is moved away from the leading edge of migrating cells through its connection to moving dorsal actin cables, and the absence of connections to immobile ventral stress fibers. It is unclear how these asymmetric nucleo-cytoskeleton connections are established. Here, using an in vitro wound assay, we find that remodeling of endoplasmic reticulum (ER) impacts nuclear positioning through the formation of a barrier that shields immobile ventral stress fibers. The remodeling of ER and perinuclear ER accumulation is mediated by the ER shaping protein Climp-63. Furthermore, ectopic recruitment of the ER to stress fibers restores nuclear positioning in the absence of Climp-63. Our findings suggest that the ER mediates asymmetric nucleo-cytoskeleton connections to position the nucleus.This work was supported by the European Research Council H2020-GA 810207-ARPCOMPLEXITY and FP7-GA 617676 PHONICS (ERG), EMBO installation (ERG), Marine Biological Laboratory Whitman Center Fellowship (ERG), Association pour la Recherche sur le Cancer and La Ligue Nacionale contre le Cancer (ERG) and Fundação para a Ciência e a Tecnologia (SFRH/BD/112286/2015)(CJ), European Research Council H2020-GA 679368 AXIAL.EC (CF); Fundação para a Ciência e Tecnologia (PTDC/MED-PAT/31639/2017; CEECIND/04251/2017)(CF) and Fondation LeDucq (17CVD03)(CF).Springer NatureRepositório da Universidade de LisboaJanota, CátiaPinto, AndreiaPezzarossa, AnnaMachado, PedroCosta, JuditeCampinho, PedroFranco, ClaudioGomes, Edgar2022-05-24T14:30:56Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/53158engNat Commun. 2022 May 19;13(1):276310.1038/s41467-022-30388-32041-1723info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:58:49Zoai:repositorio.ul.pt:10451/53158Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:04:07.912995Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Shielding of actin by the endoplasmic reticulum impacts nuclear positioning |
title |
Shielding of actin by the endoplasmic reticulum impacts nuclear positioning |
spellingShingle |
Shielding of actin by the endoplasmic reticulum impacts nuclear positioning Janota, Cátia |
title_short |
Shielding of actin by the endoplasmic reticulum impacts nuclear positioning |
title_full |
Shielding of actin by the endoplasmic reticulum impacts nuclear positioning |
title_fullStr |
Shielding of actin by the endoplasmic reticulum impacts nuclear positioning |
title_full_unstemmed |
Shielding of actin by the endoplasmic reticulum impacts nuclear positioning |
title_sort |
Shielding of actin by the endoplasmic reticulum impacts nuclear positioning |
author |
Janota, Cátia |
author_facet |
Janota, Cátia Pinto, Andreia Pezzarossa, Anna Machado, Pedro Costa, Judite Campinho, Pedro Franco, Claudio Gomes, Edgar |
author_role |
author |
author2 |
Pinto, Andreia Pezzarossa, Anna Machado, Pedro Costa, Judite Campinho, Pedro Franco, Claudio Gomes, Edgar |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Janota, Cátia Pinto, Andreia Pezzarossa, Anna Machado, Pedro Costa, Judite Campinho, Pedro Franco, Claudio Gomes, Edgar |
description |
© The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-24T14:30:56Z 2022 2022-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/53158 |
url |
http://hdl.handle.net/10451/53158 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Nat Commun. 2022 May 19;13(1):2763 10.1038/s41467-022-30388-3 2041-1723 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Springer Nature |
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Springer Nature |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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