A Biocontrol in vitro of Trichoderma spp. to pathogens Rhizoctonia solani, Fusarium oxysporium and, Curvularia lunata
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.19084/rca.21282 |
Resumo: | Fungus of the genera Rhizoctonia, Fusarium, Curvularia is among the main fungi that cause disease in plants. Trichoderma fungi are an important tool in the biological control of phytopathogens. As a result, the present study aimed to evaluate the efficiency of in vitro antagonism of Trichoderma species to the pathogens Rhizoctonia solani, Fusarium oxysporium, Curvularia lunata. To test the action of Trichoderma spp. the colony matching technique was used and the percentage of colonization, inhibition, and scores assigned on an adapted scale were evaluated. For inhibition by volatile compounds, the plate overlap method was used, and the colony diameter was measured after seven days. The experimental design was completely randomized in triplicate and analyzed in a 4 x 3 factorial scheme, with 4 isolated and 3 pathogens. In the inhibition by volatile compounds, the UFT-204 isolate showed better efficiency in controlling pathogens. In the colony matching to F. oxysporum, the UFT-25 and UFT-204 isolates showed greater antagonistic action. The UFT-25 and UFT-205 isolates provided greater antagonism in colonization and inhibition of C. lunata. All isolates were efficient in controlling R. solani. |
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A Biocontrol in vitro of Trichoderma spp. to pathogens Rhizoctonia solani, Fusarium oxysporium and, Curvularia lunataA Biocontrol in vitro of Trichoderma spp. for pathogens Rhizoctonia solani, Fusarium oxysporum, and Curvularia lunataGeralFungus of the genera Rhizoctonia, Fusarium, Curvularia is among the main fungi that cause disease in plants. Trichoderma fungi are an important tool in the biological control of phytopathogens. As a result, the present study aimed to evaluate the efficiency of in vitro antagonism of Trichoderma species to the pathogens Rhizoctonia solani, Fusarium oxysporium, Curvularia lunata. To test the action of Trichoderma spp. the colony matching technique was used and the percentage of colonization, inhibition, and scores assigned on an adapted scale were evaluated. For inhibition by volatile compounds, the plate overlap method was used, and the colony diameter was measured after seven days. The experimental design was completely randomized in triplicate and analyzed in a 4 x 3 factorial scheme, with 4 isolated and 3 pathogens. In the inhibition by volatile compounds, the UFT-204 isolate showed better efficiency in controlling pathogens. In the colony matching to F. oxysporum, the UFT-25 and UFT-204 isolates showed greater antagonistic action. The UFT-25 and UFT-205 isolates provided greater antagonism in colonization and inhibition of C. lunata. All isolates were efficient in controlling R. solani.Rhizoctonia, Fusarium, and Curvularia species are among the main fungi that cause disease in plants. Trichoderma spp. fungi are an important tool in the biological control of phytopathogens. As a result, the present study aimed to evaluate the efficiency of Trichoderma species in vitro antagonism for pathogens Rhizoctonia solani, Fusarium oxysporum, and Curvularia lunata. To test the action of Trichoderma spp. the colony matching technique was used and the percentage of colonization, inhibition, and scores assigned on an adapted scale were evaluated. For inhibition by volatile compounds, the plate overlap method was used and the colony diameter was measured after seven days. The experimental design was completely randomized in triplicate and analysed in a 4x3 factorial scheme, with 4 isolated and 3 pathogens. In the inhibition by volatile compounds, the UFT-204 isolate showed better efficiency in controlling pathogens. The colonies of the pathogens obtained mycelial growth of 74.7% for R. solani, 57.5% for F. oxysporum, and 69% for C. lunata. In the confrontation with F. oxysporum, the isolate Trichoderma UFT-205 obtained the best result (80.8%) in the percentage of colonization. In the percentage of inhibition, the isolates of Trichoderma UFT-25 (87.0%), UFT-205 (85.3%), and UFT-57 (84.2%) presented better averages. For C. lunata, the isolates of Trichoderma UFT-25 (77%), UFT-205 (81.4%), and UFT-57 (76%) provided the highest percentage of colonization. In the inhibition, the isolates of Trichoderma UFT-25 (76.3%) and UFT-205 (80.7%) presented better averages. R. solani did not differ statistically, obtaining a colonization percentage of 68.8% to 76.7% and inhibition of 76.7% to 84.6%.Sociedade de Ciências Agrárias de Portugal2021-05-25T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://doi.org/10.19084/rca.21282eng2183-041X0871-018XOliveira, Rodrigo Silva daMartins, AdilonMartins, Albert Lennon LimaNunes, Helber VerasNunes, Bruno Henrique di NapoliChagas, Lillian França BorgesChagas Júnior, Aloisio Freitasinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-06T09:25:27Zoai:ojs.revistas.rcaap.pt:article/21282Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:31:24.682285Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A Biocontrol in vitro of Trichoderma spp. to pathogens Rhizoctonia solani, Fusarium oxysporium and, Curvularia lunata A Biocontrol in vitro of Trichoderma spp. for pathogens Rhizoctonia solani, Fusarium oxysporum, and Curvularia lunata |
title |
A Biocontrol in vitro of Trichoderma spp. to pathogens Rhizoctonia solani, Fusarium oxysporium and, Curvularia lunata |
spellingShingle |
A Biocontrol in vitro of Trichoderma spp. to pathogens Rhizoctonia solani, Fusarium oxysporium and, Curvularia lunata Oliveira, Rodrigo Silva da Geral |
title_short |
A Biocontrol in vitro of Trichoderma spp. to pathogens Rhizoctonia solani, Fusarium oxysporium and, Curvularia lunata |
title_full |
A Biocontrol in vitro of Trichoderma spp. to pathogens Rhizoctonia solani, Fusarium oxysporium and, Curvularia lunata |
title_fullStr |
A Biocontrol in vitro of Trichoderma spp. to pathogens Rhizoctonia solani, Fusarium oxysporium and, Curvularia lunata |
title_full_unstemmed |
A Biocontrol in vitro of Trichoderma spp. to pathogens Rhizoctonia solani, Fusarium oxysporium and, Curvularia lunata |
title_sort |
A Biocontrol in vitro of Trichoderma spp. to pathogens Rhizoctonia solani, Fusarium oxysporium and, Curvularia lunata |
author |
Oliveira, Rodrigo Silva da |
author_facet |
Oliveira, Rodrigo Silva da Martins, Adilon Martins, Albert Lennon Lima Nunes, Helber Veras Nunes, Bruno Henrique di Napoli Chagas, Lillian França Borges Chagas Júnior, Aloisio Freitas |
author_role |
author |
author2 |
Martins, Adilon Martins, Albert Lennon Lima Nunes, Helber Veras Nunes, Bruno Henrique di Napoli Chagas, Lillian França Borges Chagas Júnior, Aloisio Freitas |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Rodrigo Silva da Martins, Adilon Martins, Albert Lennon Lima Nunes, Helber Veras Nunes, Bruno Henrique di Napoli Chagas, Lillian França Borges Chagas Júnior, Aloisio Freitas |
dc.subject.por.fl_str_mv |
Geral |
topic |
Geral |
description |
Fungus of the genera Rhizoctonia, Fusarium, Curvularia is among the main fungi that cause disease in plants. Trichoderma fungi are an important tool in the biological control of phytopathogens. As a result, the present study aimed to evaluate the efficiency of in vitro antagonism of Trichoderma species to the pathogens Rhizoctonia solani, Fusarium oxysporium, Curvularia lunata. To test the action of Trichoderma spp. the colony matching technique was used and the percentage of colonization, inhibition, and scores assigned on an adapted scale were evaluated. For inhibition by volatile compounds, the plate overlap method was used, and the colony diameter was measured after seven days. The experimental design was completely randomized in triplicate and analyzed in a 4 x 3 factorial scheme, with 4 isolated and 3 pathogens. In the inhibition by volatile compounds, the UFT-204 isolate showed better efficiency in controlling pathogens. In the colony matching to F. oxysporum, the UFT-25 and UFT-204 isolates showed greater antagonistic action. The UFT-25 and UFT-205 isolates provided greater antagonism in colonization and inhibition of C. lunata. All isolates were efficient in controlling R. solani. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-05-25T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.19084/rca.21282 |
url |
https://doi.org/10.19084/rca.21282 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2183-041X 0871-018X |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Sociedade de Ciências Agrárias de Portugal |
publisher.none.fl_str_mv |
Sociedade de Ciências Agrárias de Portugal |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799130179308617728 |