The bacterial MrpORP is a novel Mrp/NBP35 protein involved in iron-sulfur biogenesis

Detalhes bibliográficos
Autor(a) principal: Pardoux, Romain
Data de Publicação: 2019
Outros Autores: Fiévet, Anouchka, Carreira, Cíntia, Brochier-Armanet, Céline, Valette, Odile, Dermoun, Zorah, Py, Béatrice, Dolla, Alain, Pauleta, Sofia R., Aubert, Corinne
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1038/s41598-018-37021-8
Resumo: The French National Research Agency (ANR) funded this research project (ANR-12-ISV8-0003-01). This work was supported by Fundacao para a Ciencia e Tecnologia (FCT) (grant to SRP, FCT-ANR/BBB-MET/0023/2012, and to CC, SFRH/BD/87898/2012) and by the Belgian Federal Science Policy Office (Belspo) (grant to BD, IAP7/44, iPROS project). Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO was financed by national funds from FCT/MEC (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). This article is based upon work from COST Action CA15133, supported by COST (European Cooperation in Science and Technology).
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spelling The bacterial MrpORP is a novel Mrp/NBP35 protein involved in iron-sulfur biogenesisGeneralThe French National Research Agency (ANR) funded this research project (ANR-12-ISV8-0003-01). This work was supported by Fundacao para a Ciencia e Tecnologia (FCT) (grant to SRP, FCT-ANR/BBB-MET/0023/2012, and to CC, SFRH/BD/87898/2012) and by the Belgian Federal Science Policy Office (Belspo) (grant to BD, IAP7/44, iPROS project). Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO was financed by national funds from FCT/MEC (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). This article is based upon work from COST Action CA15133, supported by COST (European Cooperation in Science and Technology).Despite recent advances in understanding the biogenesis of iron-sulfur (Fe-S) proteins, most studies focused on aerobic bacteria as model organisms. Accordingly, multiple players have been proposed to participate in the Fe-S delivery step to apo-target proteins, but critical gaps exist in the knowledge of Fe-S proteins biogenesis in anaerobic organisms. Mrp/NBP35 ATP-binding proteins are a subclass of the soluble P-loop containing nucleoside triphosphate hydrolase superfamily (P-loop NTPase) known to bind and transfer Fe-S clusters in vitro. Here, we report investigations of a novel atypical two-domain Mrp/NBP35 ATP-binding protein named MrpORP associating a P-loop NTPase domain with a dinitrogenase iron-molybdenum cofactor biosynthesis domain (Di-Nase). Characterization of full length MrpORP, as well as of its two domains, showed that both domains bind Fe-S clusters. We provide in vitro evidence that the P-loop NTPase domain of the MrpORP can efficiently transfer its Fe-S cluster to apo-target proteins of the ORange Protein (ORP) complex, suggesting that this novel protein is involved in the maturation of these Fe-S proteins. Last, we showed for the first time, by fluorescence microscopy imaging a polar localization of a Mrp/NBP35 protein.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNPardoux, RomainFiévet, AnouchkaCarreira, CíntiaBrochier-Armanet, CélineValette, OdileDermoun, ZorahPy, BéatriceDolla, AlainPauleta, Sofia R.Aubert, Corinne2019-07-17T22:41:06Z2019-12-012019-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1038/s41598-018-37021-8eng2045-2322PURE: 12002713http://www.scopus.com/inward/record.url?scp=85060516481&partnerID=8YFLogxKhttps://doi.org/10.1038/s41598-018-37021-8info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:34:39Zoai:run.unl.pt:10362/75788Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:35:34.213462Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The bacterial MrpORP is a novel Mrp/NBP35 protein involved in iron-sulfur biogenesis
title The bacterial MrpORP is a novel Mrp/NBP35 protein involved in iron-sulfur biogenesis
spellingShingle The bacterial MrpORP is a novel Mrp/NBP35 protein involved in iron-sulfur biogenesis
Pardoux, Romain
General
title_short The bacterial MrpORP is a novel Mrp/NBP35 protein involved in iron-sulfur biogenesis
title_full The bacterial MrpORP is a novel Mrp/NBP35 protein involved in iron-sulfur biogenesis
title_fullStr The bacterial MrpORP is a novel Mrp/NBP35 protein involved in iron-sulfur biogenesis
title_full_unstemmed The bacterial MrpORP is a novel Mrp/NBP35 protein involved in iron-sulfur biogenesis
title_sort The bacterial MrpORP is a novel Mrp/NBP35 protein involved in iron-sulfur biogenesis
author Pardoux, Romain
author_facet Pardoux, Romain
Fiévet, Anouchka
Carreira, Cíntia
Brochier-Armanet, Céline
Valette, Odile
Dermoun, Zorah
Py, Béatrice
Dolla, Alain
Pauleta, Sofia R.
Aubert, Corinne
author_role author
author2 Fiévet, Anouchka
Carreira, Cíntia
Brochier-Armanet, Céline
Valette, Odile
Dermoun, Zorah
Py, Béatrice
Dolla, Alain
Pauleta, Sofia R.
Aubert, Corinne
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Pardoux, Romain
Fiévet, Anouchka
Carreira, Cíntia
Brochier-Armanet, Céline
Valette, Odile
Dermoun, Zorah
Py, Béatrice
Dolla, Alain
Pauleta, Sofia R.
Aubert, Corinne
dc.subject.por.fl_str_mv General
topic General
description The French National Research Agency (ANR) funded this research project (ANR-12-ISV8-0003-01). This work was supported by Fundacao para a Ciencia e Tecnologia (FCT) (grant to SRP, FCT-ANR/BBB-MET/0023/2012, and to CC, SFRH/BD/87898/2012) and by the Belgian Federal Science Policy Office (Belspo) (grant to BD, IAP7/44, iPROS project). Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO was financed by national funds from FCT/MEC (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). This article is based upon work from COST Action CA15133, supported by COST (European Cooperation in Science and Technology).
publishDate 2019
dc.date.none.fl_str_mv 2019-07-17T22:41:06Z
2019-12-01
2019-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1038/s41598-018-37021-8
url https://doi.org/10.1038/s41598-018-37021-8
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2045-2322
PURE: 12002713
http://www.scopus.com/inward/record.url?scp=85060516481&partnerID=8YFLogxK
https://doi.org/10.1038/s41598-018-37021-8
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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