Understanding the aspartic proteases role in grapevine resistance against Plasmopara viticola

Detalhes bibliográficos
Autor(a) principal: Figueiredo, Laura Alexandra Pereira
Data de Publicação: 2021
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/23525
Resumo: Mestrado em Biologia dos Recursos Vegetais / Instituto Superior de Agronomia. Universidade de Lisboa
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spelling Understanding the aspartic proteases role in grapevine resistance against Plasmopara viticolaplant-pathogen interactionVitis viniferadowny mildewdefense MechanismsproteasesMestrado em Biologia dos Recursos Vegetais / Instituto Superior de Agronomia. Universidade de LisboaGrapevine (Vitis vinifera L.) is one of the most important crops worldwide used mostly in wine production. Vitis vinifera cultivars are highly susceptible to downy mildew caused by the oomycete Plasmopara viticola (Berk. & M. A. Curtis) Berl. & De Toni. The damage caused by downy mildew is controlled with the intensive use of chemicals. Viticulturists and the scientific community are aiming for an optimization and reduction of pesticide usage in order to follow European Union guidelines. In Portugal, if the active substances at risk of exclusion by the EU are removed, it is estimated that downy mildew may cause a decline of 46% in the final production of wine grapes. To achieve a more sustainable viticulture practice, it is essential to understand this specific pathosystem. During plant-pathogen interaction, proteases play different roles in several layers of the activation of the immune response. The aim of this study was to identify and characterize the involvement of aspartic proteases (APs) in grapevine defense against P. viticola. We have conducted a genome wide search and bioinformatics characterization of V. vinifera AP gene family. A total of 81 APs proteins coded by 65 APs genes were found in V. vinifera genome. To investigate the phylogenetic relationship, we built a phylogenetic tree with 81 VviAPs and APs of other model plant species. Our results indicate that VviAPs can be divided into three categories, similar to those previously described for other plant models. Gene expression analysis of 12 selected VviAPs genes was conducted by quantitative real time PCR to further evaluate their role in the grapevine-P. viticola pathosystem. In the incompatible interaction, 5 VviAPs were significantly up-regulated, suggesting that VviAPs may have a role in grapevine defense against P. viticolaISA/ULSantos, Ana Rita BasílioRomeiras, Maria ManuelRepositório da Universidade de LisboaFigueiredo, Laura Alexandra Pereira2023-02-01T01:30:27Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.5/23525TID:203085388engFigueiredo, L.A.P. - Understanding the aspartic proteases role in grapevine resistance against Plasmopara viticola. Lisboa: ISA, 2021, 104 p.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:53:06Zoai:www.repository.utl.pt:10400.5/23525Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:07:44.565921Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Understanding the aspartic proteases role in grapevine resistance against Plasmopara viticola
title Understanding the aspartic proteases role in grapevine resistance against Plasmopara viticola
spellingShingle Understanding the aspartic proteases role in grapevine resistance against Plasmopara viticola
Figueiredo, Laura Alexandra Pereira
plant-pathogen interaction
Vitis vinifera
downy mildew
defense Mechanisms
proteases
title_short Understanding the aspartic proteases role in grapevine resistance against Plasmopara viticola
title_full Understanding the aspartic proteases role in grapevine resistance against Plasmopara viticola
title_fullStr Understanding the aspartic proteases role in grapevine resistance against Plasmopara viticola
title_full_unstemmed Understanding the aspartic proteases role in grapevine resistance against Plasmopara viticola
title_sort Understanding the aspartic proteases role in grapevine resistance against Plasmopara viticola
author Figueiredo, Laura Alexandra Pereira
author_facet Figueiredo, Laura Alexandra Pereira
author_role author
dc.contributor.none.fl_str_mv Santos, Ana Rita Basílio
Romeiras, Maria Manuel
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Figueiredo, Laura Alexandra Pereira
dc.subject.por.fl_str_mv plant-pathogen interaction
Vitis vinifera
downy mildew
defense Mechanisms
proteases
topic plant-pathogen interaction
Vitis vinifera
downy mildew
defense Mechanisms
proteases
description Mestrado em Biologia dos Recursos Vegetais / Instituto Superior de Agronomia. Universidade de Lisboa
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01T00:00:00Z
2023-02-01T01:30:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/23525
TID:203085388
url http://hdl.handle.net/10400.5/23525
identifier_str_mv TID:203085388
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Figueiredo, L.A.P. - Understanding the aspartic proteases role in grapevine resistance against Plasmopara viticola. Lisboa: ISA, 2021, 104 p.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ISA/UL
publisher.none.fl_str_mv ISA/UL
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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