Evaluation of the Effects of Exposure to Urban Particulate Matter (PM) on Marine Organisms
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/155195 |
Resumo: | Atmospheric particulate matter (PM) is a significant threat, as it contains pollutants that can cause damage to the environment and especially to the aquatic biota. This thesis falls within the scope of the research project "AMBIEnCE" and explores how pollutants from wet precipitation of atmospheric PM cause toxicity in marine organisms (Dicentrarchus labrax, Mytilus galloprovincialis, and Palaemon varians). The assays included exposure to different concentrations of suspended PM (5.7 and 11.4 mg/L). After 7, 14 and 21 days, for analyses, selected organs were sampled (muscle, liver/ digestive gland, gills, and intestines/viscera), depending on the target species. An additional trial was performed to study the eventual trophic transfer of PM by feeding seabass with PM-contaminated Polychaetes After the exposure assays, several oxidative stress biomarkers were analysed (CAT, SOD, GPX, and GST), lipid peroxidation (MDA content) and total ubiquitin, which allowed the assessment of the response to exposure to the different concentrations of PM-tested. Overall, seabass showed increased enzyme activities in the exposed animals exposed, especially those exposed to 5.7 mg/L compared to respective controls. In the seabass contaminated via diet, higher enzymatic activity was observed in muscle and liver compared to the respective controls. An increase in oxidative stress enzymes was also observed in mussels and shrimp exposed to PM. The MDA and ubiquitin levels provided information on cellular and protein damage. Although variable results, an increase in MDA and ubiquitin levels was evident in the gills of exposed mussels. Nonetheless, the higher mortality observed in fish and shrimps exposed to the highest PM concentration suggests a severe effect on these species. Overall, these results show that PM exposure can significantly impact marine organisms with increasing PM concentration and exposure time. Additionally, it provides valuable information for the risk assessment of marine ecosystems. |
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Evaluation of the Effects of Exposure to Urban Particulate Matter (PM) on Marine OrganismsParticulate Matter (PM)Estuarine organismsOxidative stressBiomarkersDomínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e TecnologiasAtmospheric particulate matter (PM) is a significant threat, as it contains pollutants that can cause damage to the environment and especially to the aquatic biota. This thesis falls within the scope of the research project "AMBIEnCE" and explores how pollutants from wet precipitation of atmospheric PM cause toxicity in marine organisms (Dicentrarchus labrax, Mytilus galloprovincialis, and Palaemon varians). The assays included exposure to different concentrations of suspended PM (5.7 and 11.4 mg/L). After 7, 14 and 21 days, for analyses, selected organs were sampled (muscle, liver/ digestive gland, gills, and intestines/viscera), depending on the target species. An additional trial was performed to study the eventual trophic transfer of PM by feeding seabass with PM-contaminated Polychaetes After the exposure assays, several oxidative stress biomarkers were analysed (CAT, SOD, GPX, and GST), lipid peroxidation (MDA content) and total ubiquitin, which allowed the assessment of the response to exposure to the different concentrations of PM-tested. Overall, seabass showed increased enzyme activities in the exposed animals exposed, especially those exposed to 5.7 mg/L compared to respective controls. In the seabass contaminated via diet, higher enzymatic activity was observed in muscle and liver compared to the respective controls. An increase in oxidative stress enzymes was also observed in mussels and shrimp exposed to PM. The MDA and ubiquitin levels provided information on cellular and protein damage. Although variable results, an increase in MDA and ubiquitin levels was evident in the gills of exposed mussels. Nonetheless, the higher mortality observed in fish and shrimps exposed to the highest PM concentration suggests a severe effect on these species. Overall, these results show that PM exposure can significantly impact marine organisms with increasing PM concentration and exposure time. Additionally, it provides valuable information for the risk assessment of marine ecosystems.O material particulado atmosférico (PM) é uma ameaça significativa, pois contém poluentes que podem causar danos no ambiente e principalmente no biota aquático. Esta tese enquadra-se no âmbito do projeto de investigação "AMBIEnCE" e explora como os poluentes da precipitação húmida de PM atmosféricos causam toxicidade em organismos marinhos (Dicentrarchus labrax, Mytilus gallopro-vincialis e Palaemon variantes). Os ensaios incluíram a exposição a diferentes concentrações de PM em suspensão (5,7 e 11,4 mg/L). Após 7, 14 e 21 dias, para as análises, foram amostrados vários órgãos (músculo, fígado/glândula digestiva, brânquias e intestinos), selecionados de acordo com a espécie alvo. Um ensaio adicional foi realizado para estudar a eventual transferência trófica de PM alimentando robalos com Polychaetes contaminados com PM. Após os ensaios de exposição, vários biomarcadores de stress oxidativo foram analisados (CAT, SOD, GPX e GST), peroxidação lipídica (níveis de MDA) e ubiquitina total, o que permitiu avaliar a resposta à exposição às diferentes concentrações de PM testadas. No geral, o robalo mostrou atividades enzimáticas aumentadas nos animais expostos, especialmente naqueles expostos a 5,7 mg/L em comparação com os respetivos controlos. Nos robalos contaminados através da dieta, observou-se maior atividade enzimática no músculo e fígado em comparação com os respetivos controlos. Um aumento nas enzimas do estresse oxidativo também foi observado nos mexilhões e camarões expostos às diferentes concentrações de PM. Os níveis de MDA e ubiquitina forneceram informações sobre danos celulares e proteicos. Embora se tenha observado que os resultados obtidos apresentaram alguma variabilidade, um aumento nos níveis de MDA e ubiquitina foi evidente nas brânquias dos mexilhões expostos a PM. No entanto, foi o aumento da mortalidade observada em peixes e camarões expostos à maior concentração de PM que sugeriu um efeito severo da exposição a PM. No geral, os resultados mostram que a exposição ao PM tem um impacto significativo nos organismos marinhos com o aumento da concentração de PM e do tempo de exposição. Além disso, fornece informações valiosas de avaliação de risco para os ecossistemas marinhos.Diniz, MárioDuarte, ReginaRUNRodrigues, Inês Araújo2023-07-13T08:13:42Z2022-122022-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/155195enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:37:44Zoai:run.unl.pt:10362/155195Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:55:58.211820Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Evaluation of the Effects of Exposure to Urban Particulate Matter (PM) on Marine Organisms |
title |
Evaluation of the Effects of Exposure to Urban Particulate Matter (PM) on Marine Organisms |
spellingShingle |
Evaluation of the Effects of Exposure to Urban Particulate Matter (PM) on Marine Organisms Rodrigues, Inês Araújo Particulate Matter (PM) Estuarine organisms Oxidative stress Biomarkers Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias |
title_short |
Evaluation of the Effects of Exposure to Urban Particulate Matter (PM) on Marine Organisms |
title_full |
Evaluation of the Effects of Exposure to Urban Particulate Matter (PM) on Marine Organisms |
title_fullStr |
Evaluation of the Effects of Exposure to Urban Particulate Matter (PM) on Marine Organisms |
title_full_unstemmed |
Evaluation of the Effects of Exposure to Urban Particulate Matter (PM) on Marine Organisms |
title_sort |
Evaluation of the Effects of Exposure to Urban Particulate Matter (PM) on Marine Organisms |
author |
Rodrigues, Inês Araújo |
author_facet |
Rodrigues, Inês Araújo |
author_role |
author |
dc.contributor.none.fl_str_mv |
Diniz, Mário Duarte, Regina RUN |
dc.contributor.author.fl_str_mv |
Rodrigues, Inês Araújo |
dc.subject.por.fl_str_mv |
Particulate Matter (PM) Estuarine organisms Oxidative stress Biomarkers Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias |
topic |
Particulate Matter (PM) Estuarine organisms Oxidative stress Biomarkers Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias |
description |
Atmospheric particulate matter (PM) is a significant threat, as it contains pollutants that can cause damage to the environment and especially to the aquatic biota. This thesis falls within the scope of the research project "AMBIEnCE" and explores how pollutants from wet precipitation of atmospheric PM cause toxicity in marine organisms (Dicentrarchus labrax, Mytilus galloprovincialis, and Palaemon varians). The assays included exposure to different concentrations of suspended PM (5.7 and 11.4 mg/L). After 7, 14 and 21 days, for analyses, selected organs were sampled (muscle, liver/ digestive gland, gills, and intestines/viscera), depending on the target species. An additional trial was performed to study the eventual trophic transfer of PM by feeding seabass with PM-contaminated Polychaetes After the exposure assays, several oxidative stress biomarkers were analysed (CAT, SOD, GPX, and GST), lipid peroxidation (MDA content) and total ubiquitin, which allowed the assessment of the response to exposure to the different concentrations of PM-tested. Overall, seabass showed increased enzyme activities in the exposed animals exposed, especially those exposed to 5.7 mg/L compared to respective controls. In the seabass contaminated via diet, higher enzymatic activity was observed in muscle and liver compared to the respective controls. An increase in oxidative stress enzymes was also observed in mussels and shrimp exposed to PM. The MDA and ubiquitin levels provided information on cellular and protein damage. Although variable results, an increase in MDA and ubiquitin levels was evident in the gills of exposed mussels. Nonetheless, the higher mortality observed in fish and shrimps exposed to the highest PM concentration suggests a severe effect on these species. Overall, these results show that PM exposure can significantly impact marine organisms with increasing PM concentration and exposure time. Additionally, it provides valuable information for the risk assessment of marine ecosystems. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12 2022-12-01T00:00:00Z 2023-07-13T08:13:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/155195 |
url |
http://hdl.handle.net/10362/155195 |
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eng |
language |
eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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