Metabolism and occurrence of methanogenic and sulfate-reducing syntrophic acetate oxidizing communities in haloalkaline environments

Detalhes bibliográficos
Autor(a) principal: Timmers, Peer H. A.
Data de Publicação: 2018
Outros Autores: Vavourakis, Charlotte D., Kleerebezem, Robbert, Damste, Jaap S. Sinninghe, Muyzer, Gerard, Stams, Alfons Johannes Maria, Sorokin, Dimitry Y., Plugge, Caroline M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/57412
Resumo: The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb. 2018.03039/full#supplementary-material
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spelling Metabolism and occurrence of methanogenic and sulfate-reducing syntrophic acetate oxidizing communities in haloalkaline environmentssyntrophic acetate oxidationhaloalkaliphilessoda lakessyntrophySAOBsyntrophic acetate oxidizing bacteriagenome-centric metagenomicsScience & TechnologyThe Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb. 2018.03039/full#supplementary-materialAnaerobic syntrophic acetate oxidation (SAO) is a thermodynamically unfavorable process involving a syntrophic acetate oxidizing bacterium (SAOB) that forms interspecies electron carriers (IECs). These IECs are consumed by syntrophic partners, typically hydrogenotrophic methanogenic archaea or sulfate reducing bacteria. In this work, the metabolism and occurrence of SAOB at extremely haloalkaline conditions were investigated, using highly enriched methanogenic (M-SAO) and sulfate-reducing (S-SAO) cultures from south-eastern Siberian hypersaline soda lakes. Activity tests with the M-SAO and S-SAO cultures and thermodynamic calculations indicated that hydrogen and formate are important IECs in both SAO cultures. Metagenomic analysis of the M-SAO cultures showed that the dominant SAOB was Candidatus Syntrophonatronum acetioxidans, and a near-complete draft genome of this SAOB was reconstructed. Ca. S. acetioxidans has all genes necessary for operating the Wood-Ljungdahl pathway, which is likely employed for acetate oxidation. It also encodes several genes essential to thrive at haloalkaline conditions; including a Na+-dependent ATP synthase and marker genes for salt-out strategies for osmotic homeostasis at high soda conditions. Membrane lipid analysis of the M-SAO culture showed the presence of unusual bacterial diether membrane lipids which are presumably beneficial at extreme haloalkaline conditions. To determine the importance of SAO in haloalkaline environments, previously obtained 16S rRNA gene sequencing data and metagenomic data of five different hypersaline soda lake sediment samples were investigated, including the soda lakes where the enrichment cultures originated from. The draft genome of Ca. S. acetioxidans showed highest identity with two metagenome-assembled genomes (MAGs) of putative SAOBs that belonged to the highly abundant and diverse Syntrophomonadaceae family present in the soda lake sediments. The 16S rRNA amplicon datasets of the soda lake sediments showed a high similarity of reads to Ca. S. acetioxidans with abundance as high as 1.3% of all reads, whereas aceticlastic methanogens and acetate oxidizing sulfate-reducers were not abundant (0.1%) or could not be detected. These combined results indicate that SAO is the primary anaerobic acetate oxidizing pathway at extreme haloalkaline conditions performed by haloalkaliphilic syntrophic consortia.This research was supported by the Soehngen Institute of AnaerobicMicrobiology(SIAM) Gravitation grant(024.002.002) of the Netherlands Ministry of Education, Culture and Science and the Netherlands Organisation for Scientific Research (NWO). GM and CV were supported by the ERC Advanced Grant PARASOL (No. 322551). DS also received support from the Russian Foundation for Basic Research (16-04-00035) and the Russian Academy of Sciences and Federal Agency of Scientific Organizations(0104-2018-0033), AS by the ERC Advanced Grant Novel Anaerobes (No. 323009), and JD by the ERC Advanced Grant Microlipids (No.694569).info:eu-repo/semantics/publishedVersionFrontiers MediaUniversidade do MinhoTimmers, Peer H. A.Vavourakis, Charlotte D.Kleerebezem, RobbertDamste, Jaap S. SinningheMuyzer, GerardStams, Alfons Johannes MariaSorokin, Dimitry Y.Plugge, Caroline M.2018-122018-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/57412engTimmers, Peer; Vavourakis, Charlotte; Kleerebezem, Robbert; Damste, Jaap S. S.; Muyzer, Gerard; Stams, A. J. M.; Sorokin, Dimitry Y.; Plugge, Caroline M., Metabolism and occurrence of methanogenic and sulfate-reducing syntrophic acetate oxidizing communities in haloalkaline environments. Frontiers in Microbiology, 9, 3039, 20181664-302X10.3389/fmicb.2018.03039http://journal.frontiersin.org/journal/microbiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:24:22Zoai:repositorium.sdum.uminho.pt:1822/57412Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:18:21.951585Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Metabolism and occurrence of methanogenic and sulfate-reducing syntrophic acetate oxidizing communities in haloalkaline environments
title Metabolism and occurrence of methanogenic and sulfate-reducing syntrophic acetate oxidizing communities in haloalkaline environments
spellingShingle Metabolism and occurrence of methanogenic and sulfate-reducing syntrophic acetate oxidizing communities in haloalkaline environments
Timmers, Peer H. A.
syntrophic acetate oxidation
haloalkaliphiles
soda lakes
syntrophy
SAOB
syntrophic acetate oxidizing bacteria
genome-centric metagenomics
Science & Technology
title_short Metabolism and occurrence of methanogenic and sulfate-reducing syntrophic acetate oxidizing communities in haloalkaline environments
title_full Metabolism and occurrence of methanogenic and sulfate-reducing syntrophic acetate oxidizing communities in haloalkaline environments
title_fullStr Metabolism and occurrence of methanogenic and sulfate-reducing syntrophic acetate oxidizing communities in haloalkaline environments
title_full_unstemmed Metabolism and occurrence of methanogenic and sulfate-reducing syntrophic acetate oxidizing communities in haloalkaline environments
title_sort Metabolism and occurrence of methanogenic and sulfate-reducing syntrophic acetate oxidizing communities in haloalkaline environments
author Timmers, Peer H. A.
author_facet Timmers, Peer H. A.
Vavourakis, Charlotte D.
Kleerebezem, Robbert
Damste, Jaap S. Sinninghe
Muyzer, Gerard
Stams, Alfons Johannes Maria
Sorokin, Dimitry Y.
Plugge, Caroline M.
author_role author
author2 Vavourakis, Charlotte D.
Kleerebezem, Robbert
Damste, Jaap S. Sinninghe
Muyzer, Gerard
Stams, Alfons Johannes Maria
Sorokin, Dimitry Y.
Plugge, Caroline M.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Timmers, Peer H. A.
Vavourakis, Charlotte D.
Kleerebezem, Robbert
Damste, Jaap S. Sinninghe
Muyzer, Gerard
Stams, Alfons Johannes Maria
Sorokin, Dimitry Y.
Plugge, Caroline M.
dc.subject.por.fl_str_mv syntrophic acetate oxidation
haloalkaliphiles
soda lakes
syntrophy
SAOB
syntrophic acetate oxidizing bacteria
genome-centric metagenomics
Science & Technology
topic syntrophic acetate oxidation
haloalkaliphiles
soda lakes
syntrophy
SAOB
syntrophic acetate oxidizing bacteria
genome-centric metagenomics
Science & Technology
description The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb. 2018.03039/full#supplementary-material
publishDate 2018
dc.date.none.fl_str_mv 2018-12
2018-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/57412
url http://hdl.handle.net/1822/57412
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Timmers, Peer; Vavourakis, Charlotte; Kleerebezem, Robbert; Damste, Jaap S. S.; Muyzer, Gerard; Stams, A. J. M.; Sorokin, Dimitry Y.; Plugge, Caroline M., Metabolism and occurrence of methanogenic and sulfate-reducing syntrophic acetate oxidizing communities in haloalkaline environments. Frontiers in Microbiology, 9, 3039, 2018
1664-302X
10.3389/fmicb.2018.03039
http://journal.frontiersin.org/journal/microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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