HER2-Targeted immunotherapy and combined protocols showed promising antiproliferative effects in feline mammary carcinoma cell-based models

Detalhes bibliográficos
Autor(a) principal: Gameiro, Andreia
Data de Publicação: 2021
Outros Autores: Nascimento, Catarina, Correia, Jorge Manuel de Jesus, Ferreira, Fernando
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/23405
Resumo: Research Areas: Oncology
id RCAP_72f75e582845828d3c72bed030358ea1
oai_identifier_str oai:www.repository.utl.pt:10400.5/23405
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling HER2-Targeted immunotherapy and combined protocols showed promising antiproliferative effects in feline mammary carcinoma cell-based modelsFeline mammary carcinomaHER2Monoclonal antibodiesCombined therapiesTyrosineKinase inhibitorsFeline her2 mutationsResearch Areas: OncologyABSTRACT - Feline mammary carcinoma (FMC) is a highly prevalent tumor, showing aggressive clinicopathological features, with HER2-positive being the most frequent subtype. While, in human breast cancer, the use of anti-HER2 monoclonal antibodies (mAbs) is common, acting by blocking the extracellular domain (ECD) of the HER2 protein and by inducing cell apoptosis, scarce information is available on use these immunoagents in FMC. Thus, the antiproliferative effects of two mAbs (trastuzumab and pertuzumab), of an antibody–drug conjugate compound (T-DM1) and of combined treatments with a tyrosine kinase inhibitor (lapatinib) were evaluated on three FMC cell lines (CATMT, FMCm and FMCp). In parallel, the DNA sequence of the her2 ECD (subdomains II and IV) was analyzed in 40 clinical samples of FMC, in order to identify mutations, which can lead to antibody resistance or be used as prognostic biomarkers. Results obtained revealed a strong antiproliferative effect in all feline cell lines, and a synergistic response was observed when combined therapies were performed. Additionally, the mutations found were not described as inducing resistance to therapy in breast cancer patients. Altogether, our results suggested that anti-HER2 mAbs could become useful in the treatment of FMC, particularly, if combined with lapatinib, since drug-resistance seems to be rare.MDPIRepositório da Universidade de LisboaGameiro, AndreiaNascimento, CatarinaCorreia, Jorge Manuel de JesusFerreira, Fernando2022-02-05T22:52:11Z2021-04-212021-04-21T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/23405engGameiro A, Nascimento C, Correia J, Ferreira F. 2021. HER2-Targeted immunotherapy and combined protocols showed promising antiproliferative effects in feline mammary carcinoma cell-based models. Cancers 13(9):2007. Doi: 10.3390/cancers1309200710.3390/cancers130920072072-6694info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:52:58Zoai:www.repository.utl.pt:10400.5/23405Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:07:39.028965Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv HER2-Targeted immunotherapy and combined protocols showed promising antiproliferative effects in feline mammary carcinoma cell-based models
title HER2-Targeted immunotherapy and combined protocols showed promising antiproliferative effects in feline mammary carcinoma cell-based models
spellingShingle HER2-Targeted immunotherapy and combined protocols showed promising antiproliferative effects in feline mammary carcinoma cell-based models
Gameiro, Andreia
Feline mammary carcinoma
HER2
Monoclonal antibodies
Combined therapies
Tyrosine
Kinase inhibitors
Feline her2 mutations
title_short HER2-Targeted immunotherapy and combined protocols showed promising antiproliferative effects in feline mammary carcinoma cell-based models
title_full HER2-Targeted immunotherapy and combined protocols showed promising antiproliferative effects in feline mammary carcinoma cell-based models
title_fullStr HER2-Targeted immunotherapy and combined protocols showed promising antiproliferative effects in feline mammary carcinoma cell-based models
title_full_unstemmed HER2-Targeted immunotherapy and combined protocols showed promising antiproliferative effects in feline mammary carcinoma cell-based models
title_sort HER2-Targeted immunotherapy and combined protocols showed promising antiproliferative effects in feline mammary carcinoma cell-based models
author Gameiro, Andreia
author_facet Gameiro, Andreia
Nascimento, Catarina
Correia, Jorge Manuel de Jesus
Ferreira, Fernando
author_role author
author2 Nascimento, Catarina
Correia, Jorge Manuel de Jesus
Ferreira, Fernando
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Gameiro, Andreia
Nascimento, Catarina
Correia, Jorge Manuel de Jesus
Ferreira, Fernando
dc.subject.por.fl_str_mv Feline mammary carcinoma
HER2
Monoclonal antibodies
Combined therapies
Tyrosine
Kinase inhibitors
Feline her2 mutations
topic Feline mammary carcinoma
HER2
Monoclonal antibodies
Combined therapies
Tyrosine
Kinase inhibitors
Feline her2 mutations
description Research Areas: Oncology
publishDate 2021
dc.date.none.fl_str_mv 2021-04-21
2021-04-21T00:00:00Z
2022-02-05T22:52:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/23405
url http://hdl.handle.net/10400.5/23405
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Gameiro A, Nascimento C, Correia J, Ferreira F. 2021. HER2-Targeted immunotherapy and combined protocols showed promising antiproliferative effects in feline mammary carcinoma cell-based models. Cancers 13(9):2007. Doi: 10.3390/cancers13092007
10.3390/cancers13092007
2072-6694
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799131170982592512

Registros relacionados