M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in brain tissue
Autor(a) principal: | |
---|---|
Data de Publicação: | 2024 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/88858 |
Resumo: | Oligomeric clusters of amyloid- (A) are one of the major biomarkers for Alzheimers disease (AD). However, proficient methods to detect A-oligomers in brain tissue are lacking. Here we show that synthetic M13 bacteriophages displaying A-derived peptides on their surface preferentially interact with A-oligomers. When exposed to brain tissue isolated from APP/PS1-transgenic mice, these bacteriophages detect small-sized A-aggregates in hippocampus at an early age, prior to the occurrence of A-plaques. Similarly, the bacteriophages reveal the presence of such small A-aggregates in post-mortem hippocampus tissue of ADpatients. These results advocate bacteriophages displaying A-peptides as a convenient and low-cost tool to identify A-oligomers in post-mortem brain tissue of AD-model mice and AD patients. |
id |
RCAP_730af87dcfe307b5e6e6beb4e4410038 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/88858 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in brain tissueOligomeric clusters of amyloid- (A) are one of the major biomarkers for Alzheimers disease (AD). However, proficient methods to detect A-oligomers in brain tissue are lacking. Here we show that synthetic M13 bacteriophages displaying A-derived peptides on their surface preferentially interact with A-oligomers. When exposed to brain tissue isolated from APP/PS1-transgenic mice, these bacteriophages detect small-sized A-aggregates in hippocampus at an early age, prior to the occurrence of A-plaques. Similarly, the bacteriophages reveal the presence of such small A-aggregates in post-mortem hippocampus tissue of ADpatients. These results advocate bacteriophages displaying A-peptides as a convenient and low-cost tool to identify A-oligomers in post-mortem brain tissue of AD-model mice and AD patients.FCT -Fundação para a Ciência e a Tecnologia(SFRH/BD/101171/2014)info:eu-repo/semantics/publishedVersionSpringer NatureUniversidade do MinhoMartins, Ivone M.Lima, Alexandrede Graaff, WimCristóvão, Joana S.Brosens, NiekAronica, EleonoraKluskens, LeonGomes, Cláudio M.Azeredo, JoanaKessels, Helmut W.20242024-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/88858engMartins, I.M., Lima, A., de Graaff, W. et al. M13 phage grafted with peptide motifs as a tool to detect amyloid-β oligomers in brain tissue. Commun Biol 7, 134 (2024). https://doi.org/10.1038/s42003-024-05806-52399-364210.1038/s42003-024-05806-538280942https://www.nature.com/articles/s42003-024-05806-5info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-24T01:26:17Zoai:repositorium.sdum.uminho.pt:1822/88858Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:11:11.111126Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in brain tissue |
title |
M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in brain tissue |
spellingShingle |
M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in brain tissue Martins, Ivone M. |
title_short |
M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in brain tissue |
title_full |
M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in brain tissue |
title_fullStr |
M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in brain tissue |
title_full_unstemmed |
M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in brain tissue |
title_sort |
M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in brain tissue |
author |
Martins, Ivone M. |
author_facet |
Martins, Ivone M. Lima, Alexandre de Graaff, Wim Cristóvão, Joana S. Brosens, Niek Aronica, Eleonora Kluskens, Leon Gomes, Cláudio M. Azeredo, Joana Kessels, Helmut W. |
author_role |
author |
author2 |
Lima, Alexandre de Graaff, Wim Cristóvão, Joana S. Brosens, Niek Aronica, Eleonora Kluskens, Leon Gomes, Cláudio M. Azeredo, Joana Kessels, Helmut W. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Martins, Ivone M. Lima, Alexandre de Graaff, Wim Cristóvão, Joana S. Brosens, Niek Aronica, Eleonora Kluskens, Leon Gomes, Cláudio M. Azeredo, Joana Kessels, Helmut W. |
description |
Oligomeric clusters of amyloid- (A) are one of the major biomarkers for Alzheimers disease (AD). However, proficient methods to detect A-oligomers in brain tissue are lacking. Here we show that synthetic M13 bacteriophages displaying A-derived peptides on their surface preferentially interact with A-oligomers. When exposed to brain tissue isolated from APP/PS1-transgenic mice, these bacteriophages detect small-sized A-aggregates in hippocampus at an early age, prior to the occurrence of A-plaques. Similarly, the bacteriophages reveal the presence of such small A-aggregates in post-mortem hippocampus tissue of ADpatients. These results advocate bacteriophages displaying A-peptides as a convenient and low-cost tool to identify A-oligomers in post-mortem brain tissue of AD-model mice and AD patients. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024 2024-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/88858 |
url |
https://hdl.handle.net/1822/88858 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Martins, I.M., Lima, A., de Graaff, W. et al. M13 phage grafted with peptide motifs as a tool to detect amyloid-β oligomers in brain tissue. Commun Biol 7, 134 (2024). https://doi.org/10.1038/s42003-024-05806-5 2399-3642 10.1038/s42003-024-05806-5 38280942 https://www.nature.com/articles/s42003-024-05806-5 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Nature |
publisher.none.fl_str_mv |
Springer Nature |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799137761706377216 |