M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in brain tissue

Detalhes bibliográficos
Autor(a) principal: Martins, Ivone M.
Data de Publicação: 2024
Outros Autores: Lima, Alexandre, de Graaff, Wim, Cristóvão, Joana S., Brosens, Niek, Aronica, Eleonora, Kluskens, Leon, Gomes, Cláudio M., Azeredo, Joana, Kessels, Helmut W.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/88858
Resumo: Oligomeric clusters of amyloid- (A) are one of the major biomarkers for Alzheimers disease (AD). However, proficient methods to detect A-oligomers in brain tissue are lacking. Here we show that synthetic M13 bacteriophages displaying A-derived peptides on their surface preferentially interact with A-oligomers. When exposed to brain tissue isolated from APP/PS1-transgenic mice, these bacteriophages detect small-sized A-aggregates in hippocampus at an early age, prior to the occurrence of A-plaques. Similarly, the bacteriophages reveal the presence of such small A-aggregates in post-mortem hippocampus tissue of ADpatients. These results advocate bacteriophages displaying A-peptides as a convenient and low-cost tool to identify A-oligomers in post-mortem brain tissue of AD-model mice and AD patients.
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spelling M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in brain tissueOligomeric clusters of amyloid- (A) are one of the major biomarkers for Alzheimers disease (AD). However, proficient methods to detect A-oligomers in brain tissue are lacking. Here we show that synthetic M13 bacteriophages displaying A-derived peptides on their surface preferentially interact with A-oligomers. When exposed to brain tissue isolated from APP/PS1-transgenic mice, these bacteriophages detect small-sized A-aggregates in hippocampus at an early age, prior to the occurrence of A-plaques. Similarly, the bacteriophages reveal the presence of such small A-aggregates in post-mortem hippocampus tissue of ADpatients. These results advocate bacteriophages displaying A-peptides as a convenient and low-cost tool to identify A-oligomers in post-mortem brain tissue of AD-model mice and AD patients.FCT -Fundação para a Ciência e a Tecnologia(SFRH/BD/101171/2014)info:eu-repo/semantics/publishedVersionSpringer NatureUniversidade do MinhoMartins, Ivone M.Lima, Alexandrede Graaff, WimCristóvão, Joana S.Brosens, NiekAronica, EleonoraKluskens, LeonGomes, Cláudio M.Azeredo, JoanaKessels, Helmut W.20242024-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/88858engMartins, I.M., Lima, A., de Graaff, W. et al. M13 phage grafted with peptide motifs as a tool to detect amyloid-β oligomers in brain tissue. Commun Biol 7, 134 (2024). https://doi.org/10.1038/s42003-024-05806-52399-364210.1038/s42003-024-05806-538280942https://www.nature.com/articles/s42003-024-05806-5info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-24T01:26:17Zoai:repositorium.sdum.uminho.pt:1822/88858Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:11:11.111126Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in brain tissue
title M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in brain tissue
spellingShingle M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in brain tissue
Martins, Ivone M.
title_short M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in brain tissue
title_full M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in brain tissue
title_fullStr M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in brain tissue
title_full_unstemmed M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in brain tissue
title_sort M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in brain tissue
author Martins, Ivone M.
author_facet Martins, Ivone M.
Lima, Alexandre
de Graaff, Wim
Cristóvão, Joana S.
Brosens, Niek
Aronica, Eleonora
Kluskens, Leon
Gomes, Cláudio M.
Azeredo, Joana
Kessels, Helmut W.
author_role author
author2 Lima, Alexandre
de Graaff, Wim
Cristóvão, Joana S.
Brosens, Niek
Aronica, Eleonora
Kluskens, Leon
Gomes, Cláudio M.
Azeredo, Joana
Kessels, Helmut W.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Martins, Ivone M.
Lima, Alexandre
de Graaff, Wim
Cristóvão, Joana S.
Brosens, Niek
Aronica, Eleonora
Kluskens, Leon
Gomes, Cláudio M.
Azeredo, Joana
Kessels, Helmut W.
description Oligomeric clusters of amyloid- (A) are one of the major biomarkers for Alzheimers disease (AD). However, proficient methods to detect A-oligomers in brain tissue are lacking. Here we show that synthetic M13 bacteriophages displaying A-derived peptides on their surface preferentially interact with A-oligomers. When exposed to brain tissue isolated from APP/PS1-transgenic mice, these bacteriophages detect small-sized A-aggregates in hippocampus at an early age, prior to the occurrence of A-plaques. Similarly, the bacteriophages reveal the presence of such small A-aggregates in post-mortem hippocampus tissue of ADpatients. These results advocate bacteriophages displaying A-peptides as a convenient and low-cost tool to identify A-oligomers in post-mortem brain tissue of AD-model mice and AD patients.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/88858
url https://hdl.handle.net/1822/88858
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Martins, I.M., Lima, A., de Graaff, W. et al. M13 phage grafted with peptide motifs as a tool to detect amyloid-β oligomers in brain tissue. Commun Biol 7, 134 (2024). https://doi.org/10.1038/s42003-024-05806-5
2399-3642
10.1038/s42003-024-05806-5
38280942
https://www.nature.com/articles/s42003-024-05806-5
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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