Therapeutic Potential of Deflamin against Colorectal Cancer Development and Progression

Detalhes bibliográficos
Autor(a) principal: Silva, Sara
Data de Publicação: 2022
Outros Autores: Cavaco, Ana, Basso, Bianca, Mota, Joana, Cruz-Duarte, Raquel, Costa, Miguel, Carvalho, Lara, Lima, Ana, Costa, Luís, Ferreira, Ricardo, Martins, Marta
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/27237
Resumo: Matrix metalloproteinases (MMPs) are proteolytic enzymes that play a crucial role in tumor microenvironment remodeling, contributing to inflammatory and angiogenic processes, and ultimately promoting tumor maintenance and progression. Several studies on bioactive polypeptides isolated from legumes have shown anti-migratory, anti-MMPs, and anti-tumor effects, potentially constituting novel strategies for both the prevention and progression of cancer. In this work, we inves- tigated the anti-tumor role of deflamin, a protein oligomer isolated from white lupine seeds (Lupinus albus) reported to inhibit MMP-9 and cell migration in colorectal cancer (CRC) cell lines. We found that deflamin exerts an inhibitory effect on tumor growth and metastasis formation, contributing to increased tumor apoptosis in the xenotransplanted zebrafish larvae model. Furthermore, deflamin resulted not only in a significant reduction in MMP-2 and MMP-9 activity but also in impaired cancer cell migration and invasion in vitro. Using the xenograft zebrafish model, we observed that deflamin inhibits collagen degradation and angiogenesis in the tumor microenvironment in vivo. Overall, our work reveals the potential of deflamin as an agent against CRC development and progression.
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spelling Therapeutic Potential of Deflamin against Colorectal Cancer Development and Progressiondeflamincolorectal cancerMMP-2MMP-9Matrix metalloproteinases (MMPs) are proteolytic enzymes that play a crucial role in tumor microenvironment remodeling, contributing to inflammatory and angiogenic processes, and ultimately promoting tumor maintenance and progression. Several studies on bioactive polypeptides isolated from legumes have shown anti-migratory, anti-MMPs, and anti-tumor effects, potentially constituting novel strategies for both the prevention and progression of cancer. In this work, we inves- tigated the anti-tumor role of deflamin, a protein oligomer isolated from white lupine seeds (Lupinus albus) reported to inhibit MMP-9 and cell migration in colorectal cancer (CRC) cell lines. We found that deflamin exerts an inhibitory effect on tumor growth and metastasis formation, contributing to increased tumor apoptosis in the xenotransplanted zebrafish larvae model. Furthermore, deflamin resulted not only in a significant reduction in MMP-2 and MMP-9 activity but also in impaired cancer cell migration and invasion in vitro. Using the xenograft zebrafish model, we observed that deflamin inhibits collagen degradation and angiogenesis in the tumor microenvironment in vivo. Overall, our work reveals the potential of deflamin as an agent against CRC development and progression.MDPIRepositório da Universidade de LisboaSilva, SaraCavaco, AnaBasso, BiancaMota, JoanaCruz-Duarte, RaquelCosta, MiguelCarvalho, LaraLima, AnaCosta, LuísFerreira, RicardoMartins, Marta2023-02-10T19:36:07Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/27237engSilva, S.; Cavaco, A.; Basso, B.; Mota, J.; Cruz-Duarte, R.; Costa, M.; Carvalho, L.; Lima, A.; Costa, L.; Ferreira, R.; et al. Therapeutic Potential of Deflamin against Colorectal Cancer Development and Progression. Cancers 2022, 14, 6182. https://doi.org/10.3390/ cancers1424618210.3390/ cancers14246182info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:56:37Zoai:www.repository.utl.pt:10400.5/27237Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:10:45.798852Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Therapeutic Potential of Deflamin against Colorectal Cancer Development and Progression
title Therapeutic Potential of Deflamin against Colorectal Cancer Development and Progression
spellingShingle Therapeutic Potential of Deflamin against Colorectal Cancer Development and Progression
Silva, Sara
deflamin
colorectal cancer
MMP-2
MMP-9
title_short Therapeutic Potential of Deflamin against Colorectal Cancer Development and Progression
title_full Therapeutic Potential of Deflamin against Colorectal Cancer Development and Progression
title_fullStr Therapeutic Potential of Deflamin against Colorectal Cancer Development and Progression
title_full_unstemmed Therapeutic Potential of Deflamin against Colorectal Cancer Development and Progression
title_sort Therapeutic Potential of Deflamin against Colorectal Cancer Development and Progression
author Silva, Sara
author_facet Silva, Sara
Cavaco, Ana
Basso, Bianca
Mota, Joana
Cruz-Duarte, Raquel
Costa, Miguel
Carvalho, Lara
Lima, Ana
Costa, Luís
Ferreira, Ricardo
Martins, Marta
author_role author
author2 Cavaco, Ana
Basso, Bianca
Mota, Joana
Cruz-Duarte, Raquel
Costa, Miguel
Carvalho, Lara
Lima, Ana
Costa, Luís
Ferreira, Ricardo
Martins, Marta
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Silva, Sara
Cavaco, Ana
Basso, Bianca
Mota, Joana
Cruz-Duarte, Raquel
Costa, Miguel
Carvalho, Lara
Lima, Ana
Costa, Luís
Ferreira, Ricardo
Martins, Marta
dc.subject.por.fl_str_mv deflamin
colorectal cancer
MMP-2
MMP-9
topic deflamin
colorectal cancer
MMP-2
MMP-9
description Matrix metalloproteinases (MMPs) are proteolytic enzymes that play a crucial role in tumor microenvironment remodeling, contributing to inflammatory and angiogenic processes, and ultimately promoting tumor maintenance and progression. Several studies on bioactive polypeptides isolated from legumes have shown anti-migratory, anti-MMPs, and anti-tumor effects, potentially constituting novel strategies for both the prevention and progression of cancer. In this work, we inves- tigated the anti-tumor role of deflamin, a protein oligomer isolated from white lupine seeds (Lupinus albus) reported to inhibit MMP-9 and cell migration in colorectal cancer (CRC) cell lines. We found that deflamin exerts an inhibitory effect on tumor growth and metastasis formation, contributing to increased tumor apoptosis in the xenotransplanted zebrafish larvae model. Furthermore, deflamin resulted not only in a significant reduction in MMP-2 and MMP-9 activity but also in impaired cancer cell migration and invasion in vitro. Using the xenograft zebrafish model, we observed that deflamin inhibits collagen degradation and angiogenesis in the tumor microenvironment in vivo. Overall, our work reveals the potential of deflamin as an agent against CRC development and progression.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-01T00:00:00Z
2023-02-10T19:36:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/27237
url http://hdl.handle.net/10400.5/27237
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Silva, S.; Cavaco, A.; Basso, B.; Mota, J.; Cruz-Duarte, R.; Costa, M.; Carvalho, L.; Lima, A.; Costa, L.; Ferreira, R.; et al. Therapeutic Potential of Deflamin against Colorectal Cancer Development and Progression. Cancers 2022, 14, 6182. https://doi.org/10.3390/ cancers14246182
10.3390/ cancers14246182
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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