Exploring genetic variability of Giardia duodenalis and Enterocytozoon bieneusi in raw vegetables and fruits

Detalhes bibliográficos
Autor(a) principal: Salamandane, Cátia
Data de Publicação: 2023
Outros Autores: Lobo, Maria Luísa, Afonso, Sónia, Xiao, Lihua, Matos, Olga
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/159220
Resumo: Funding Information: This research was funded by Fundação para a Ciência e a Tecnologia (FCT) with a scholarship reference SFRH/BD/135355/2017 from TropikMan Doctoral Program, Lisboa, Portugal and the funds to GHTM – UID/Multi/04413/2019. Publisher Copyright: Copyright © 2023 Salamandane, Lobo, Afonso, Xiao and Matos.
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spelling Exploring genetic variability of Giardia duodenalis and Enterocytozoon bieneusi in raw vegetables and fruitsImplications for food safety and public health in Mozambiquefoodborne diseasesintestinal protozoaMaputomicrosporidiaOne Healthraw horticultural productszoonotic transmissionMicrobiologyMicrobiology (medical)SDG 3 - Good Health and Well-beingFunding Information: This research was funded by Fundação para a Ciência e a Tecnologia (FCT) with a scholarship reference SFRH/BD/135355/2017 from TropikMan Doctoral Program, Lisboa, Portugal and the funds to GHTM – UID/Multi/04413/2019. Publisher Copyright: Copyright © 2023 Salamandane, Lobo, Afonso, Xiao and Matos.Giardia duodenalis and Enterocytozoon bieneusi are etiological agents of enteric diseases characterized by diarrhea that can progress to chronicity in humans, especially in children and in immunocompromised patients. This study aims to assess the genetic pattern of G. duodenalis and E. bieneusi detected in vegetables and fruits commercialized in Maputo markets, Mozambique and determine their public health importance. Eight study points were sampled: a farmer zone, a wholesale, four retail markets, and two supermarkets in Maputo city, where eight types of horticultural products were purchased. Using nested-PCR methods, 2.8% (9/321) and 1.3% (4/321) of samples monitored were positive for G. duodenalis and E. bieneusi, respectively. Based on the analysis of the β-giardin and ITS rRNA sequences of G. duodenalis and E. bieneusi detected, respectively, four different sequences of G. duodenalis (three novel sequences: BgMZ1, BgMZ2, and BgMZ3, and one known sequence) all from assemblage B and three genotypes of E. bieneusi (two novel sequences: EbMZ4 and EbMZ5, and one known sequence: KIN-1) from group 1. These microorganisms were found and characterized for the first time in horticultural products in Maputo markets. All identified G. duodenalis and E. bieneusi display high genetic similarity within their β-giardin and ITS rRNA sequences, respectively, having been clustered into assemblages and genotypes with high zoonotic transmission potential. Our study may represent a relevant step in the understanding of these intestinal pathogens in association with fresh vegetables and fruits for human consumption, for a better and broader “One Health” approach.Instituto de Higiene e Medicina Tropical (IHMT)NOVA School of Business and Economics (NOVA SBE)Global Health and Tropical Medicine (GHTM)RUNSalamandane, CátiaLobo, Maria LuísaAfonso, SóniaXiao, LihuaMatos, Olga2023-10-23T22:20:24Z2023-08-042023-08-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/159220eng1664-302XPURE: 74526584https://doi.org/10.3389/fmicb.2023.1223151info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:41:39Zoai:run.unl.pt:10362/159220Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:26.976479Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Exploring genetic variability of Giardia duodenalis and Enterocytozoon bieneusi in raw vegetables and fruits
Implications for food safety and public health in Mozambique
title Exploring genetic variability of Giardia duodenalis and Enterocytozoon bieneusi in raw vegetables and fruits
spellingShingle Exploring genetic variability of Giardia duodenalis and Enterocytozoon bieneusi in raw vegetables and fruits
Salamandane, Cátia
foodborne diseases
intestinal protozoa
Maputo
microsporidia
One Health
raw horticultural products
zoonotic transmission
Microbiology
Microbiology (medical)
SDG 3 - Good Health and Well-being
title_short Exploring genetic variability of Giardia duodenalis and Enterocytozoon bieneusi in raw vegetables and fruits
title_full Exploring genetic variability of Giardia duodenalis and Enterocytozoon bieneusi in raw vegetables and fruits
title_fullStr Exploring genetic variability of Giardia duodenalis and Enterocytozoon bieneusi in raw vegetables and fruits
title_full_unstemmed Exploring genetic variability of Giardia duodenalis and Enterocytozoon bieneusi in raw vegetables and fruits
title_sort Exploring genetic variability of Giardia duodenalis and Enterocytozoon bieneusi in raw vegetables and fruits
author Salamandane, Cátia
author_facet Salamandane, Cátia
Lobo, Maria Luísa
Afonso, Sónia
Xiao, Lihua
Matos, Olga
author_role author
author2 Lobo, Maria Luísa
Afonso, Sónia
Xiao, Lihua
Matos, Olga
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Higiene e Medicina Tropical (IHMT)
NOVA School of Business and Economics (NOVA SBE)
Global Health and Tropical Medicine (GHTM)
RUN
dc.contributor.author.fl_str_mv Salamandane, Cátia
Lobo, Maria Luísa
Afonso, Sónia
Xiao, Lihua
Matos, Olga
dc.subject.por.fl_str_mv foodborne diseases
intestinal protozoa
Maputo
microsporidia
One Health
raw horticultural products
zoonotic transmission
Microbiology
Microbiology (medical)
SDG 3 - Good Health and Well-being
topic foodborne diseases
intestinal protozoa
Maputo
microsporidia
One Health
raw horticultural products
zoonotic transmission
Microbiology
Microbiology (medical)
SDG 3 - Good Health and Well-being
description Funding Information: This research was funded by Fundação para a Ciência e a Tecnologia (FCT) with a scholarship reference SFRH/BD/135355/2017 from TropikMan Doctoral Program, Lisboa, Portugal and the funds to GHTM – UID/Multi/04413/2019. Publisher Copyright: Copyright © 2023 Salamandane, Lobo, Afonso, Xiao and Matos.
publishDate 2023
dc.date.none.fl_str_mv 2023-10-23T22:20:24Z
2023-08-04
2023-08-04T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/159220
url http://hdl.handle.net/10362/159220
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1664-302X
PURE: 74526584
https://doi.org/10.3389/fmicb.2023.1223151
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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