Effects of singlet oxygen generated by a broad-spectrum viral fusion inhibitor on membrane nanoarchitecture

Detalhes bibliográficos
Autor(a) principal: Hollmann, Axel
Data de Publicação: 2015
Outros Autores: Gonçalves, Sónia, Augusto, Marcelo T., Castanho, Miguel A. R. B., Lee, Benhur, Santos, Nuno C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/23501
Resumo: © 2015 Elsevier Inc. All rights reserved.
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spelling Effects of singlet oxygen generated by a broad-spectrum viral fusion inhibitor on membrane nanoarchitectureBroad-spectrum antiviralSinglet oxygenAFMMembrane organization© 2015 Elsevier Inc. All rights reserved.Targeting membranes of enveloped viruses represents an exciting new paradigm to explore on the development of broad-spectrum antivirals. Recently, broad-spectrum small-molecule antiviral drugs were described, preventing enveloped virus entry at an intermediate step, after virus binding but before virus–cell fusion. Those compounds, including an oxazolidine-2,4-dithione named JL103 that presented the most promissing results, act deleteriously on the virus envelope but not at the cell membrane level. In this work, by using atomic force microscopy (AFM), we aimed at unraveling the effects that JL103 is able to induce in the lipid membrane architecture at the nanoscale. Our results indicate that singlet oxygen produced by JL103 decreases membrane thickness, with an expansion of the area per phospholipid, by attacking the double bonds of unsaturated phospholipids. This membrane reorganization prevents the fusion between enveloped virus and target cell membranes, resulting in viral entry inhibition.Fundação para a Ciência e Tecnologia, Ministério da Educação e Ciência (FCT-MEC, Portugal) project VIH/SAU/ 0047/2011, and fellowships SFRH/BPD/72037/2010 and SFRH/BD/95624/ 2013 to A.H. and M.T.A., respectively, as well as NIH grants U01 AI070495 and U01 AI082100. JL103 and LJ025 were generously provided by Michael E. Jung and Jihye Lee (University of California, Los Angeles)ElsevierRepositório da Universidade de LisboaHollmann, AxelGonçalves, SóniaAugusto, Marcelo T.Castanho, Miguel A. R. B.Lee, BenhurSantos, Nuno C.2016-04-21T12:45:53Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/23501engNanomedicine: Nanotechnology, Biology, and Medicine 11 (2015) 1163–11671549-963410.1016/j.nano.2015.02.014metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:11:28Zoai:repositorio.ul.pt:10451/23501Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:40:48.053937Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effects of singlet oxygen generated by a broad-spectrum viral fusion inhibitor on membrane nanoarchitecture
title Effects of singlet oxygen generated by a broad-spectrum viral fusion inhibitor on membrane nanoarchitecture
spellingShingle Effects of singlet oxygen generated by a broad-spectrum viral fusion inhibitor on membrane nanoarchitecture
Hollmann, Axel
Broad-spectrum antiviral
Singlet oxygen
AFM
Membrane organization
title_short Effects of singlet oxygen generated by a broad-spectrum viral fusion inhibitor on membrane nanoarchitecture
title_full Effects of singlet oxygen generated by a broad-spectrum viral fusion inhibitor on membrane nanoarchitecture
title_fullStr Effects of singlet oxygen generated by a broad-spectrum viral fusion inhibitor on membrane nanoarchitecture
title_full_unstemmed Effects of singlet oxygen generated by a broad-spectrum viral fusion inhibitor on membrane nanoarchitecture
title_sort Effects of singlet oxygen generated by a broad-spectrum viral fusion inhibitor on membrane nanoarchitecture
author Hollmann, Axel
author_facet Hollmann, Axel
Gonçalves, Sónia
Augusto, Marcelo T.
Castanho, Miguel A. R. B.
Lee, Benhur
Santos, Nuno C.
author_role author
author2 Gonçalves, Sónia
Augusto, Marcelo T.
Castanho, Miguel A. R. B.
Lee, Benhur
Santos, Nuno C.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Hollmann, Axel
Gonçalves, Sónia
Augusto, Marcelo T.
Castanho, Miguel A. R. B.
Lee, Benhur
Santos, Nuno C.
dc.subject.por.fl_str_mv Broad-spectrum antiviral
Singlet oxygen
AFM
Membrane organization
topic Broad-spectrum antiviral
Singlet oxygen
AFM
Membrane organization
description © 2015 Elsevier Inc. All rights reserved.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
2016-04-21T12:45:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/23501
url http://hdl.handle.net/10451/23501
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nanomedicine: Nanotechnology, Biology, and Medicine 11 (2015) 1163–1167
1549-9634
10.1016/j.nano.2015.02.014
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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