The effect of heat and salicylic acid on the DAHPS expression and activity in grape cells

Detalhes bibliográficos
Autor(a) principal: Noronha, Henrique Luís Silva
Data de Publicação: 2011
Outros Autores: Teixeira, António, Gerós, H.
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/15997
Resumo: High temperatures affect grape berry set and development, alter the normal sugar content of the fruit, and reduce the accumulation of anthocyanins. Also, peaks of high temperature may stop the ripening progress. Despite its impact in fruit and wine quality, the mechanisms contributing to the reduction of anthocyanin levels under high temperature are still elusive. Some studies indicate that this reduction is caused by a downregulation of the genes responsible for anthocyanin synthesis, while others propose that heat upregulates anthocyanin degradation. The enzyme 3-deoxy-D-arabino-heptulo-sonate 7-phosphate synthase (DAHPS), which as two isoforms in plants (DAHPS-01 cytosolic and Co2+ activated; DAHPS-02 plastidial and Mn2+ activated), catalyzes the first step of the Shikimate pathway by condensing phosphoenolpyruvate and D-erythrose 4-phosphate to form 3-deoxy-D-arabino-heptulo-sonate 7-phosphate. The final product of this pathway is phenylalanine that fuels the Flavonoid pathway responsible for the biosynthesis of anthocyanins and proanthocyanidins (condensed tannins). Despite its recognized importance, very little is known about the role of DAHPS in the synthesis and accumulation of important secondary metabolites under heat stress. In this study, we aim at the elucidation of the genetic and biochemical steps that regulate anthocyanin accumulation in grape cells under heat. Results on the effect of high temperature and salicylic acid (SA), a plant hormone involved in abiotic stress response, on the expression and biochemical activity of DAHPS in grape cell suspensions are presented and discussed.
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spelling The effect of heat and salicylic acid on the DAHPS expression and activity in grape cellsDAHPSPlantHeat stressGrape cellsHigh temperatures affect grape berry set and development, alter the normal sugar content of the fruit, and reduce the accumulation of anthocyanins. Also, peaks of high temperature may stop the ripening progress. Despite its impact in fruit and wine quality, the mechanisms contributing to the reduction of anthocyanin levels under high temperature are still elusive. Some studies indicate that this reduction is caused by a downregulation of the genes responsible for anthocyanin synthesis, while others propose that heat upregulates anthocyanin degradation. The enzyme 3-deoxy-D-arabino-heptulo-sonate 7-phosphate synthase (DAHPS), which as two isoforms in plants (DAHPS-01 cytosolic and Co2+ activated; DAHPS-02 plastidial and Mn2+ activated), catalyzes the first step of the Shikimate pathway by condensing phosphoenolpyruvate and D-erythrose 4-phosphate to form 3-deoxy-D-arabino-heptulo-sonate 7-phosphate. The final product of this pathway is phenylalanine that fuels the Flavonoid pathway responsible for the biosynthesis of anthocyanins and proanthocyanidins (condensed tannins). Despite its recognized importance, very little is known about the role of DAHPS in the synthesis and accumulation of important secondary metabolites under heat stress. In this study, we aim at the elucidation of the genetic and biochemical steps that regulate anthocyanin accumulation in grape cells under heat. Results on the effect of high temperature and salicylic acid (SA), a plant hormone involved in abiotic stress response, on the expression and biochemical activity of DAHPS in grape cell suspensions are presented and discussed.This work is supported by FCT (PTDC/AGR-ALI/100636/2008) Henrique Noronha is supported by FCT, grant ref. SFRH/BD/75257/2010Universidade do MinhoNoronha, Henrique Luís SilvaTeixeira, AntónioGerós, H.2011-12-292011-12-29T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/mswordhttp://hdl.handle.net/1822/15997enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T06:45:22Zoai:repositorium.sdum.uminho.pt:1822/15997Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T06:45:22Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The effect of heat and salicylic acid on the DAHPS expression and activity in grape cells
title The effect of heat and salicylic acid on the DAHPS expression and activity in grape cells
spellingShingle The effect of heat and salicylic acid on the DAHPS expression and activity in grape cells
Noronha, Henrique Luís Silva
DAHPS
Plant
Heat stress
Grape cells
title_short The effect of heat and salicylic acid on the DAHPS expression and activity in grape cells
title_full The effect of heat and salicylic acid on the DAHPS expression and activity in grape cells
title_fullStr The effect of heat and salicylic acid on the DAHPS expression and activity in grape cells
title_full_unstemmed The effect of heat and salicylic acid on the DAHPS expression and activity in grape cells
title_sort The effect of heat and salicylic acid on the DAHPS expression and activity in grape cells
author Noronha, Henrique Luís Silva
author_facet Noronha, Henrique Luís Silva
Teixeira, António
Gerós, H.
author_role author
author2 Teixeira, António
Gerós, H.
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Noronha, Henrique Luís Silva
Teixeira, António
Gerós, H.
dc.subject.por.fl_str_mv DAHPS
Plant
Heat stress
Grape cells
topic DAHPS
Plant
Heat stress
Grape cells
description High temperatures affect grape berry set and development, alter the normal sugar content of the fruit, and reduce the accumulation of anthocyanins. Also, peaks of high temperature may stop the ripening progress. Despite its impact in fruit and wine quality, the mechanisms contributing to the reduction of anthocyanin levels under high temperature are still elusive. Some studies indicate that this reduction is caused by a downregulation of the genes responsible for anthocyanin synthesis, while others propose that heat upregulates anthocyanin degradation. The enzyme 3-deoxy-D-arabino-heptulo-sonate 7-phosphate synthase (DAHPS), which as two isoforms in plants (DAHPS-01 cytosolic and Co2+ activated; DAHPS-02 plastidial and Mn2+ activated), catalyzes the first step of the Shikimate pathway by condensing phosphoenolpyruvate and D-erythrose 4-phosphate to form 3-deoxy-D-arabino-heptulo-sonate 7-phosphate. The final product of this pathway is phenylalanine that fuels the Flavonoid pathway responsible for the biosynthesis of anthocyanins and proanthocyanidins (condensed tannins). Despite its recognized importance, very little is known about the role of DAHPS in the synthesis and accumulation of important secondary metabolites under heat stress. In this study, we aim at the elucidation of the genetic and biochemical steps that regulate anthocyanin accumulation in grape cells under heat. Results on the effect of high temperature and salicylic acid (SA), a plant hormone involved in abiotic stress response, on the expression and biochemical activity of DAHPS in grape cell suspensions are presented and discussed.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-29
2011-12-29T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/15997
url http://hdl.handle.net/1822/15997
dc.language.iso.fl_str_mv eng
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instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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