Responding double-porous lipid membrane: Lyotropic phases in a polymer scaffold
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5846 https://doi.org/10.1016/j.bbamem.2007.10.020 |
Resumo: | The large osmotic gradient over the outermost layer of human skin implies major structural changes along the gradient, which in turn affects transport. In particular, the possibility of phase changes introduces a non-linear element to the transport behaviour. We present a novel model membrane system to be used for studying these transport mechanisms, where we use a hydrophobic porous polymer membrane as a scaffold for lipid lyotropic phases. The polymer membrane provides mechanical robustness, but also prevents defects of the lipid lyotropic phases, and it can induce an orientation of anisotropic phases. We study the location, structure and phase behaviour of the confined phases. It is shown that this model membrane system allow for accurate measurements of transport through lipid membranes in the presence of different osmotic gradients. A theoretical description is evaluated and shows that this phenomenon can be understood in terms of the proposed mechanism of phase changes. The novel double-porous lipid membrane constitutes a mechanically robust system for studies in aligned systems, which is generally very difficult to achieve. This could have large implications for studies of transport processes in, e.g. skin and other biomembrane model systems. |
id |
RCAP_75582c5eed36cbd8ea1eea02e5c173b9 |
---|---|
oai_identifier_str |
oai:estudogeral.uc.pt:10316/5846 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Responding double-porous lipid membrane: Lyotropic phases in a polymer scaffoldDiffusive transportPermeabilityOsmotic gradientPhase transformationStratum corneumResponding membraneThe large osmotic gradient over the outermost layer of human skin implies major structural changes along the gradient, which in turn affects transport. In particular, the possibility of phase changes introduces a non-linear element to the transport behaviour. We present a novel model membrane system to be used for studying these transport mechanisms, where we use a hydrophobic porous polymer membrane as a scaffold for lipid lyotropic phases. The polymer membrane provides mechanical robustness, but also prevents defects of the lipid lyotropic phases, and it can induce an orientation of anisotropic phases. We study the location, structure and phase behaviour of the confined phases. It is shown that this model membrane system allow for accurate measurements of transport through lipid membranes in the presence of different osmotic gradients. A theoretical description is evaluated and shows that this phenomenon can be understood in terms of the proposed mechanism of phase changes. The novel double-porous lipid membrane constitutes a mechanically robust system for studies in aligned systems, which is generally very difficult to achieve. This could have large implications for studies of transport processes in, e.g. skin and other biomembrane model systems.http://www.sciencedirect.com/science/article/B6T1T-4R1KVV0-1/1/11a3309ec00dad9b6758be173fe9ddc92008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5846http://hdl.handle.net/10316/5846https://doi.org/10.1016/j.bbamem.2007.10.020engBiochimica et Biophysica Acta (BBA) - Biomembranes. 1778:2 (2008) 549-558Åberg, ChristofferPairin, CécileCosta-Balogh, Fátima O.Sparr, Emmainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-11-06T16:48:49Zoai:estudogeral.uc.pt:10316/5846Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:47:19.625695Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Responding double-porous lipid membrane: Lyotropic phases in a polymer scaffold |
title |
Responding double-porous lipid membrane: Lyotropic phases in a polymer scaffold |
spellingShingle |
Responding double-porous lipid membrane: Lyotropic phases in a polymer scaffold Åberg, Christoffer Diffusive transport Permeability Osmotic gradient Phase transformation Stratum corneum Responding membrane |
title_short |
Responding double-porous lipid membrane: Lyotropic phases in a polymer scaffold |
title_full |
Responding double-porous lipid membrane: Lyotropic phases in a polymer scaffold |
title_fullStr |
Responding double-porous lipid membrane: Lyotropic phases in a polymer scaffold |
title_full_unstemmed |
Responding double-porous lipid membrane: Lyotropic phases in a polymer scaffold |
title_sort |
Responding double-porous lipid membrane: Lyotropic phases in a polymer scaffold |
author |
Åberg, Christoffer |
author_facet |
Åberg, Christoffer Pairin, Cécile Costa-Balogh, Fátima O. Sparr, Emma |
author_role |
author |
author2 |
Pairin, Cécile Costa-Balogh, Fátima O. Sparr, Emma |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Åberg, Christoffer Pairin, Cécile Costa-Balogh, Fátima O. Sparr, Emma |
dc.subject.por.fl_str_mv |
Diffusive transport Permeability Osmotic gradient Phase transformation Stratum corneum Responding membrane |
topic |
Diffusive transport Permeability Osmotic gradient Phase transformation Stratum corneum Responding membrane |
description |
The large osmotic gradient over the outermost layer of human skin implies major structural changes along the gradient, which in turn affects transport. In particular, the possibility of phase changes introduces a non-linear element to the transport behaviour. We present a novel model membrane system to be used for studying these transport mechanisms, where we use a hydrophobic porous polymer membrane as a scaffold for lipid lyotropic phases. The polymer membrane provides mechanical robustness, but also prevents defects of the lipid lyotropic phases, and it can induce an orientation of anisotropic phases. We study the location, structure and phase behaviour of the confined phases. It is shown that this model membrane system allow for accurate measurements of transport through lipid membranes in the presence of different osmotic gradients. A theoretical description is evaluated and shows that this phenomenon can be understood in terms of the proposed mechanism of phase changes. The novel double-porous lipid membrane constitutes a mechanically robust system for studies in aligned systems, which is generally very difficult to achieve. This could have large implications for studies of transport processes in, e.g. skin and other biomembrane model systems. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5846 http://hdl.handle.net/10316/5846 https://doi.org/10.1016/j.bbamem.2007.10.020 |
url |
http://hdl.handle.net/10316/5846 https://doi.org/10.1016/j.bbamem.2007.10.020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biochimica et Biophysica Acta (BBA) - Biomembranes. 1778:2 (2008) 549-558 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133750580215808 |