An image processing application for quantification of protein aggregates in Caenorhabditis elegans

Detalhes bibliográficos
Autor(a) principal: Teixeira-Castro, Andreia
Data de Publicação: 2011
Outros Autores: Dias, Nuno, Rodrigues, Pedro, Oliveira, João Filipe, F. Rodrigues, Nuno, Maciel, Patrícia, Vilaça, João L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/11110/419
Resumo: Protein aggregation became a widely accepted marker of many polyQ disorders, including Machado-Joseph disease (MJD), and is often used as readout for disease progression and development of therapeutic strategies. The lack of good platforms to rapidly quantify protein aggregates in a wide range of disease animal models prompted us to generate a novel image processing application that automatically identifies and quantifies the aggregates in a standardized and operator-independent manner. We propose here a novel image processing tool to quantify the protein aggregates in a Caenorhabditis elegans (C. elegans) model of MJD. Confocal mi-croscopy images were obtained from animals of different genetic conditions. The image processing application was developed using MeVisLab as a platform to pro-cess, analyse and visualize the images obtained from those animals. All segmenta-tion algorithms were based on intensity pixel levels.The quantification of area or numbers of aggregates per total body area, as well as the number of aggregates per animal were shown to be reliable and reproducible measures of protein aggrega-tion in C. elegans. The results obtained were consistent with the levels of aggrega-tion observed in the images. In conclusion, this novel imaging processing applica-tion allows the non-biased, reliable and high throughput quantification of protein aggregates in a C. elegans model of MJD, which may contribute to a significant improvement on the prognosis of treatment effectiveness for this group of disor-ders
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spelling An image processing application for quantification of protein aggregates in Caenorhabditis elegansC. elegansimage processingquantification of aggregatesProtein aggregation became a widely accepted marker of many polyQ disorders, including Machado-Joseph disease (MJD), and is often used as readout for disease progression and development of therapeutic strategies. The lack of good platforms to rapidly quantify protein aggregates in a wide range of disease animal models prompted us to generate a novel image processing application that automatically identifies and quantifies the aggregates in a standardized and operator-independent manner. We propose here a novel image processing tool to quantify the protein aggregates in a Caenorhabditis elegans (C. elegans) model of MJD. Confocal mi-croscopy images were obtained from animals of different genetic conditions. The image processing application was developed using MeVisLab as a platform to pro-cess, analyse and visualize the images obtained from those animals. All segmenta-tion algorithms were based on intensity pixel levels.The quantification of area or numbers of aggregates per total body area, as well as the number of aggregates per animal were shown to be reliable and reproducible measures of protein aggrega-tion in C. elegans. The results obtained were consistent with the levels of aggrega-tion observed in the images. In conclusion, this novel imaging processing applica-tion allows the non-biased, reliable and high throughput quantification of protein aggregates in a C. elegans model of MJD, which may contribute to a significant improvement on the prognosis of treatment effectiveness for this group of disor-ders2013-12-14T14:39:54Z2011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/11110/419oai:ciencipca.ipca.pt:11110/419enghttp://hdl.handle.net/11110/419metadata only accessinfo:eu-repo/semantics/openAccessTeixeira-Castro, AndreiaDias, NunoRodrigues, PedroOliveira, João FilipeF. Rodrigues, NunoMaciel, PatríciaVilaça, João L.reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-05T12:52:00Zoai:ciencipca.ipca.pt:11110/419Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:00:51.778226Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv An image processing application for quantification of protein aggregates in Caenorhabditis elegans
title An image processing application for quantification of protein aggregates in Caenorhabditis elegans
spellingShingle An image processing application for quantification of protein aggregates in Caenorhabditis elegans
Teixeira-Castro, Andreia
C. elegans
image processing
quantification of aggregates
title_short An image processing application for quantification of protein aggregates in Caenorhabditis elegans
title_full An image processing application for quantification of protein aggregates in Caenorhabditis elegans
title_fullStr An image processing application for quantification of protein aggregates in Caenorhabditis elegans
title_full_unstemmed An image processing application for quantification of protein aggregates in Caenorhabditis elegans
title_sort An image processing application for quantification of protein aggregates in Caenorhabditis elegans
author Teixeira-Castro, Andreia
author_facet Teixeira-Castro, Andreia
Dias, Nuno
Rodrigues, Pedro
Oliveira, João Filipe
F. Rodrigues, Nuno
Maciel, Patrícia
Vilaça, João L.
author_role author
author2 Dias, Nuno
Rodrigues, Pedro
Oliveira, João Filipe
F. Rodrigues, Nuno
Maciel, Patrícia
Vilaça, João L.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Teixeira-Castro, Andreia
Dias, Nuno
Rodrigues, Pedro
Oliveira, João Filipe
F. Rodrigues, Nuno
Maciel, Patrícia
Vilaça, João L.
dc.subject.por.fl_str_mv C. elegans
image processing
quantification of aggregates
topic C. elegans
image processing
quantification of aggregates
description Protein aggregation became a widely accepted marker of many polyQ disorders, including Machado-Joseph disease (MJD), and is often used as readout for disease progression and development of therapeutic strategies. The lack of good platforms to rapidly quantify protein aggregates in a wide range of disease animal models prompted us to generate a novel image processing application that automatically identifies and quantifies the aggregates in a standardized and operator-independent manner. We propose here a novel image processing tool to quantify the protein aggregates in a Caenorhabditis elegans (C. elegans) model of MJD. Confocal mi-croscopy images were obtained from animals of different genetic conditions. The image processing application was developed using MeVisLab as a platform to pro-cess, analyse and visualize the images obtained from those animals. All segmenta-tion algorithms were based on intensity pixel levels.The quantification of area or numbers of aggregates per total body area, as well as the number of aggregates per animal were shown to be reliable and reproducible measures of protein aggrega-tion in C. elegans. The results obtained were consistent with the levels of aggrega-tion observed in the images. In conclusion, this novel imaging processing applica-tion allows the non-biased, reliable and high throughput quantification of protein aggregates in a C. elegans model of MJD, which may contribute to a significant improvement on the prognosis of treatment effectiveness for this group of disor-ders
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01T00:00:00Z
2013-12-14T14:39:54Z
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