Noise-induced gastric lesions : a light and electron microscopy study of the rat gastric wall exposed to low frequency noise
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.26/9562 |
Resumo: | "Context - Only a few studies evaluated the digestive alterations caused by low frequency noise (LFN) and most focused only on mucosal alterations. Objectives - To investigate the morphological injury of LFN-exposed gastric wall, beyond the epithelial layer. Methods - Wistar rats were exposed to low frequency noise (LFN), during increasing periods, 1 to 13 weeks. A control group was kept in silence. Gastric specimens were studied using: (i) light microscopy with hematoxylin-eosin and immunostaining for collagens; (ii) transmission electron microscopy; (iii) morphometry allowing statistical analysis. Results - Submucosa of all LFN-exposed animals exhibit increased thickness with fibrous proliferation. Transmission electron microscopy showed massive collagen deposition. Immunostaining identified collagen IV as responsible for the increased thickness. Morphometry allowed the demonstration of a significant difference of thickness between control and exposed groups. Vascular alterations included: i) intima proliferation and thickening, rupture of the internal elastic lamina, thrombotic changes; ii) thickening of the media; iii) after 9 weeks of LFN-exposure, we found new formed vessel presenting tortuous and twisted. There is a significant difference of arterial wall thickness between control and exposed groups. Conclusions - Deeper layers of gastric wall undergo alterations, including fibrosis of the submucosa caused by collagen IV deposition, an early marker of neoangiogenesis. Vascular alterations included thickening and thrombotic phenomena, but also images of newly formed vessels. This study suggests that, at least in the stomach, LFN-induced fibrosis could be linked with neoangiogenesis." |
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Noise-induced gastric lesions : a light and electron microscopy study of the rat gastric wall exposed to low frequency noiseGastric mucosaPhysiopathologyNoise effectsMicroscopy, electronRats"Context - Only a few studies evaluated the digestive alterations caused by low frequency noise (LFN) and most focused only on mucosal alterations. Objectives - To investigate the morphological injury of LFN-exposed gastric wall, beyond the epithelial layer. Methods - Wistar rats were exposed to low frequency noise (LFN), during increasing periods, 1 to 13 weeks. A control group was kept in silence. Gastric specimens were studied using: (i) light microscopy with hematoxylin-eosin and immunostaining for collagens; (ii) transmission electron microscopy; (iii) morphometry allowing statistical analysis. Results - Submucosa of all LFN-exposed animals exhibit increased thickness with fibrous proliferation. Transmission electron microscopy showed massive collagen deposition. Immunostaining identified collagen IV as responsible for the increased thickness. Morphometry allowed the demonstration of a significant difference of thickness between control and exposed groups. Vascular alterations included: i) intima proliferation and thickening, rupture of the internal elastic lamina, thrombotic changes; ii) thickening of the media; iii) after 9 weeks of LFN-exposure, we found new formed vessel presenting tortuous and twisted. There is a significant difference of arterial wall thickness between control and exposed groups. Conclusions - Deeper layers of gastric wall undergo alterations, including fibrosis of the submucosa caused by collagen IV deposition, an early marker of neoangiogenesis. Vascular alterations included thickening and thrombotic phenomena, but also images of newly formed vessels. This study suggests that, at least in the stomach, LFN-induced fibrosis could be linked with neoangiogenesis."IBEPEGE, CBCD, SBMD, FBG, SBH, SOBEDRepositório ComumFonseca, JorgeMartins-dos-Santos, JoséOliveira, PedroLaranjeira, NunoÁguas, ArturCastelo-Branco, Nuno2015-08-03T10:07:47Z2012-01-01T00:00:00Z2012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.26/9562engFonseca J, Martins-dos-Santos J, Oliveira P, Laranjeira N, Aguas A, Castelo-Branco N. Noise-induced gastric lesions: a light and electron microscopy study of the rat gastric wall exposed to low frequency noise. Arq Gastroenterol. 2012 Jan-Mar;49(1):82-80004-280310.1590/S0004-28032012000100014info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-10-06T14:51:39Zoai:comum.rcaap.pt:10400.26/9562Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:06:31.814706Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Noise-induced gastric lesions : a light and electron microscopy study of the rat gastric wall exposed to low frequency noise |
title |
Noise-induced gastric lesions : a light and electron microscopy study of the rat gastric wall exposed to low frequency noise |
spellingShingle |
Noise-induced gastric lesions : a light and electron microscopy study of the rat gastric wall exposed to low frequency noise Fonseca, Jorge Gastric mucosa Physiopathology Noise effects Microscopy, electron Rats |
title_short |
Noise-induced gastric lesions : a light and electron microscopy study of the rat gastric wall exposed to low frequency noise |
title_full |
Noise-induced gastric lesions : a light and electron microscopy study of the rat gastric wall exposed to low frequency noise |
title_fullStr |
Noise-induced gastric lesions : a light and electron microscopy study of the rat gastric wall exposed to low frequency noise |
title_full_unstemmed |
Noise-induced gastric lesions : a light and electron microscopy study of the rat gastric wall exposed to low frequency noise |
title_sort |
Noise-induced gastric lesions : a light and electron microscopy study of the rat gastric wall exposed to low frequency noise |
author |
Fonseca, Jorge |
author_facet |
Fonseca, Jorge Martins-dos-Santos, José Oliveira, Pedro Laranjeira, Nuno Águas, Artur Castelo-Branco, Nuno |
author_role |
author |
author2 |
Martins-dos-Santos, José Oliveira, Pedro Laranjeira, Nuno Águas, Artur Castelo-Branco, Nuno |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Comum |
dc.contributor.author.fl_str_mv |
Fonseca, Jorge Martins-dos-Santos, José Oliveira, Pedro Laranjeira, Nuno Águas, Artur Castelo-Branco, Nuno |
dc.subject.por.fl_str_mv |
Gastric mucosa Physiopathology Noise effects Microscopy, electron Rats |
topic |
Gastric mucosa Physiopathology Noise effects Microscopy, electron Rats |
description |
"Context - Only a few studies evaluated the digestive alterations caused by low frequency noise (LFN) and most focused only on mucosal alterations. Objectives - To investigate the morphological injury of LFN-exposed gastric wall, beyond the epithelial layer. Methods - Wistar rats were exposed to low frequency noise (LFN), during increasing periods, 1 to 13 weeks. A control group was kept in silence. Gastric specimens were studied using: (i) light microscopy with hematoxylin-eosin and immunostaining for collagens; (ii) transmission electron microscopy; (iii) morphometry allowing statistical analysis. Results - Submucosa of all LFN-exposed animals exhibit increased thickness with fibrous proliferation. Transmission electron microscopy showed massive collagen deposition. Immunostaining identified collagen IV as responsible for the increased thickness. Morphometry allowed the demonstration of a significant difference of thickness between control and exposed groups. Vascular alterations included: i) intima proliferation and thickening, rupture of the internal elastic lamina, thrombotic changes; ii) thickening of the media; iii) after 9 weeks of LFN-exposure, we found new formed vessel presenting tortuous and twisted. There is a significant difference of arterial wall thickness between control and exposed groups. Conclusions - Deeper layers of gastric wall undergo alterations, including fibrosis of the submucosa caused by collagen IV deposition, an early marker of neoangiogenesis. Vascular alterations included thickening and thrombotic phenomena, but also images of newly formed vessels. This study suggests that, at least in the stomach, LFN-induced fibrosis could be linked with neoangiogenesis." |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01T00:00:00Z 2012-01-01T00:00:00Z 2015-08-03T10:07:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.26/9562 |
url |
http://hdl.handle.net/10400.26/9562 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fonseca J, Martins-dos-Santos J, Oliveira P, Laranjeira N, Aguas A, Castelo-Branco N. Noise-induced gastric lesions: a light and electron microscopy study of the rat gastric wall exposed to low frequency noise. Arq Gastroenterol. 2012 Jan-Mar;49(1):82-8 0004-2803 10.1590/S0004-28032012000100014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
IBEPEGE, CBCD, SBMD, FBG, SBH, SOBED |
publisher.none.fl_str_mv |
IBEPEGE, CBCD, SBMD, FBG, SBH, SOBED |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799129936293789696 |