Expression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in rats
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.13/3166 |
Resumo: | Correlation between intensity of free radical processes estimated by biochemiluminesce parameters, content of lipoperoxidation products, and changes of glutathione peroxidase (GP, EC 1.11.1.9) and glutathione reductase (GR, EC 1.6.4.2) activities at rats liver injury, after 12, 36, 70, 96, 110, and 125 hours & tetrachloromethane administration have been investigated. The histological examination of the liver sections of rats showed that prominent hepatocytes with marked vacuolisation and inflammatory cells which were arranged around the necrotic tissue are more at 96 h after exposure to CCl4. Moreover maximum increase in GR and GP activities, 2.1 and 2.5 times, respectively, was observed at 96 h after exposure to CCl4, what coincided with the maximum of free radical oxidation processes. Using a combination of reverse transcription and real-time polymerase chain reaction, expression of the glutathione peroxidase and glutathione reductase genes (Gpx1 and Gsr) was analyzed by the determination of their respective mRNAs in the rat liver tissue under toxic hepatitis conditions. The analyses of Gpx1 and Gsr expression revealed that the transcript levels increased in 2.5- and 3.0-folds, respectively. Western blot analysis revealed that the amounts of hepatic Gpx1 and Gsr proteins increased considerably after CCl4 administration. It can be proposed that the overexpression of these enzymes could be a mechanism of enhancement of hepatocytes tolerance to oxidative stress. |
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Expression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in ratsGlutathione peroxidaseGlutathione reductaseHepatitis in rats.Faculdade de Ciências da VidaCorrelation between intensity of free radical processes estimated by biochemiluminesce parameters, content of lipoperoxidation products, and changes of glutathione peroxidase (GP, EC 1.11.1.9) and glutathione reductase (GR, EC 1.6.4.2) activities at rats liver injury, after 12, 36, 70, 96, 110, and 125 hours & tetrachloromethane administration have been investigated. The histological examination of the liver sections of rats showed that prominent hepatocytes with marked vacuolisation and inflammatory cells which were arranged around the necrotic tissue are more at 96 h after exposure to CCl4. Moreover maximum increase in GR and GP activities, 2.1 and 2.5 times, respectively, was observed at 96 h after exposure to CCl4, what coincided with the maximum of free radical oxidation processes. Using a combination of reverse transcription and real-time polymerase chain reaction, expression of the glutathione peroxidase and glutathione reductase genes (Gpx1 and Gsr) was analyzed by the determination of their respective mRNAs in the rat liver tissue under toxic hepatitis conditions. The analyses of Gpx1 and Gsr expression revealed that the transcript levels increased in 2.5- and 3.0-folds, respectively. Western blot analysis revealed that the amounts of hepatic Gpx1 and Gsr proteins increased considerably after CCl4 administration. It can be proposed that the overexpression of these enzymes could be a mechanism of enhancement of hepatocytes tolerance to oxidative stress.Hindawi Publishing CorporationDigitUMaIskusnykh, Igor Y.Popova, Tatyana N.Agarkov, Aleksander A.Carvalho, Miguel Â. A. Pinheiro deRjevskiy, Stanislav G.2021-03-12T14:26:59Z2013-01-01T00:00:00Z2013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/3166engIskusnykh, I. Y., Popova, T. N., Agarkov, A. A., Carvalho, M. Â.P., & Rjevskiy, S. G. (2013). Expression of glutathione peroxidase and glutathione reductase and level of free radical processes under toxic hepatitis in rats. Journal of Toxicology, 2013, 277-285. http://dx.doi.org/10.1155/2013/87062810.1155/2013/870628info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-05T12:56:05Zoai:digituma.uma.pt:10400.13/3166Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:06:06.955208Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Expression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in rats |
title |
Expression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in rats |
spellingShingle |
Expression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in rats Iskusnykh, Igor Y. Glutathione peroxidase Glutathione reductase Hepatitis in rats . Faculdade de Ciências da Vida |
title_short |
Expression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in rats |
title_full |
Expression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in rats |
title_fullStr |
Expression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in rats |
title_full_unstemmed |
Expression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in rats |
title_sort |
Expression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in rats |
author |
Iskusnykh, Igor Y. |
author_facet |
Iskusnykh, Igor Y. Popova, Tatyana N. Agarkov, Aleksander A. Carvalho, Miguel Â. A. Pinheiro de Rjevskiy, Stanislav G. |
author_role |
author |
author2 |
Popova, Tatyana N. Agarkov, Aleksander A. Carvalho, Miguel Â. A. Pinheiro de Rjevskiy, Stanislav G. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
DigitUMa |
dc.contributor.author.fl_str_mv |
Iskusnykh, Igor Y. Popova, Tatyana N. Agarkov, Aleksander A. Carvalho, Miguel Â. A. Pinheiro de Rjevskiy, Stanislav G. |
dc.subject.por.fl_str_mv |
Glutathione peroxidase Glutathione reductase Hepatitis in rats . Faculdade de Ciências da Vida |
topic |
Glutathione peroxidase Glutathione reductase Hepatitis in rats . Faculdade de Ciências da Vida |
description |
Correlation between intensity of free radical processes estimated by biochemiluminesce parameters, content of lipoperoxidation products, and changes of glutathione peroxidase (GP, EC 1.11.1.9) and glutathione reductase (GR, EC 1.6.4.2) activities at rats liver injury, after 12, 36, 70, 96, 110, and 125 hours & tetrachloromethane administration have been investigated. The histological examination of the liver sections of rats showed that prominent hepatocytes with marked vacuolisation and inflammatory cells which were arranged around the necrotic tissue are more at 96 h after exposure to CCl4. Moreover maximum increase in GR and GP activities, 2.1 and 2.5 times, respectively, was observed at 96 h after exposure to CCl4, what coincided with the maximum of free radical oxidation processes. Using a combination of reverse transcription and real-time polymerase chain reaction, expression of the glutathione peroxidase and glutathione reductase genes (Gpx1 and Gsr) was analyzed by the determination of their respective mRNAs in the rat liver tissue under toxic hepatitis conditions. The analyses of Gpx1 and Gsr expression revealed that the transcript levels increased in 2.5- and 3.0-folds, respectively. Western blot analysis revealed that the amounts of hepatic Gpx1 and Gsr proteins increased considerably after CCl4 administration. It can be proposed that the overexpression of these enzymes could be a mechanism of enhancement of hepatocytes tolerance to oxidative stress. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01T00:00:00Z 2013-01-01T00:00:00Z 2021-03-12T14:26:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.13/3166 |
url |
http://hdl.handle.net/10400.13/3166 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Iskusnykh, I. Y., Popova, T. N., Agarkov, A. A., Carvalho, M. Â.P., & Rjevskiy, S. G. (2013). Expression of glutathione peroxidase and glutathione reductase and level of free radical processes under toxic hepatitis in rats. Journal of Toxicology, 2013, 277-285. http://dx.doi.org/10.1155/2013/870628 10.1155/2013/870628 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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