Expression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in rats

Detalhes bibliográficos
Autor(a) principal: Iskusnykh, Igor Y.
Data de Publicação: 2013
Outros Autores: Popova, Tatyana N., Agarkov, Aleksander A., Carvalho, Miguel Â. A. Pinheiro de, Rjevskiy, Stanislav G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.13/3166
Resumo: Correlation between intensity of free radical processes estimated by biochemiluminesce parameters, content of lipoperoxidation products, and changes of glutathione peroxidase (GP, EC 1.11.1.9) and glutathione reductase (GR, EC 1.6.4.2) activities at rats liver injury, after 12, 36, 70, 96, 110, and 125 hours & tetrachloromethane administration have been investigated. The histological examination of the liver sections of rats showed that prominent hepatocytes with marked vacuolisation and inflammatory cells which were arranged around the necrotic tissue are more at 96 h after exposure to CCl4. Moreover maximum increase in GR and GP activities, 2.1 and 2.5 times, respectively, was observed at 96 h after exposure to CCl4, what coincided with the maximum of free radical oxidation processes. Using a combination of reverse transcription and real-time polymerase chain reaction, expression of the glutathione peroxidase and glutathione reductase genes (Gpx1 and Gsr) was analyzed by the determination of their respective mRNAs in the rat liver tissue under toxic hepatitis conditions. The analyses of Gpx1 and Gsr expression revealed that the transcript levels increased in 2.5- and 3.0-folds, respectively. Western blot analysis revealed that the amounts of hepatic Gpx1 and Gsr proteins increased considerably after CCl4 administration. It can be proposed that the overexpression of these enzymes could be a mechanism of enhancement of hepatocytes tolerance to oxidative stress.
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spelling Expression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in ratsGlutathione peroxidaseGlutathione reductaseHepatitis in rats.Faculdade de Ciências da VidaCorrelation between intensity of free radical processes estimated by biochemiluminesce parameters, content of lipoperoxidation products, and changes of glutathione peroxidase (GP, EC 1.11.1.9) and glutathione reductase (GR, EC 1.6.4.2) activities at rats liver injury, after 12, 36, 70, 96, 110, and 125 hours & tetrachloromethane administration have been investigated. The histological examination of the liver sections of rats showed that prominent hepatocytes with marked vacuolisation and inflammatory cells which were arranged around the necrotic tissue are more at 96 h after exposure to CCl4. Moreover maximum increase in GR and GP activities, 2.1 and 2.5 times, respectively, was observed at 96 h after exposure to CCl4, what coincided with the maximum of free radical oxidation processes. Using a combination of reverse transcription and real-time polymerase chain reaction, expression of the glutathione peroxidase and glutathione reductase genes (Gpx1 and Gsr) was analyzed by the determination of their respective mRNAs in the rat liver tissue under toxic hepatitis conditions. The analyses of Gpx1 and Gsr expression revealed that the transcript levels increased in 2.5- and 3.0-folds, respectively. Western blot analysis revealed that the amounts of hepatic Gpx1 and Gsr proteins increased considerably after CCl4 administration. It can be proposed that the overexpression of these enzymes could be a mechanism of enhancement of hepatocytes tolerance to oxidative stress.Hindawi Publishing CorporationDigitUMaIskusnykh, Igor Y.Popova, Tatyana N.Agarkov, Aleksander A.Carvalho, Miguel Â. A. Pinheiro deRjevskiy, Stanislav G.2021-03-12T14:26:59Z2013-01-01T00:00:00Z2013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/3166engIskusnykh, I. Y., Popova, T. N., Agarkov, A. A., Carvalho, M. Â.P., & Rjevskiy, S. G. (2013). Expression of glutathione peroxidase and glutathione reductase and level of free radical processes under toxic hepatitis in rats. Journal of Toxicology, 2013, 277-285. http://dx.doi.org/10.1155/2013/87062810.1155/2013/870628info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-05T12:56:05Zoai:digituma.uma.pt:10400.13/3166Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:06:06.955208Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Expression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in rats
title Expression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in rats
spellingShingle Expression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in rats
Iskusnykh, Igor Y.
Glutathione peroxidase
Glutathione reductase
Hepatitis in rats
.
Faculdade de Ciências da Vida
title_short Expression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in rats
title_full Expression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in rats
title_fullStr Expression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in rats
title_full_unstemmed Expression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in rats
title_sort Expression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in rats
author Iskusnykh, Igor Y.
author_facet Iskusnykh, Igor Y.
Popova, Tatyana N.
Agarkov, Aleksander A.
Carvalho, Miguel Â. A. Pinheiro de
Rjevskiy, Stanislav G.
author_role author
author2 Popova, Tatyana N.
Agarkov, Aleksander A.
Carvalho, Miguel Â. A. Pinheiro de
Rjevskiy, Stanislav G.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv DigitUMa
dc.contributor.author.fl_str_mv Iskusnykh, Igor Y.
Popova, Tatyana N.
Agarkov, Aleksander A.
Carvalho, Miguel Â. A. Pinheiro de
Rjevskiy, Stanislav G.
dc.subject.por.fl_str_mv Glutathione peroxidase
Glutathione reductase
Hepatitis in rats
.
Faculdade de Ciências da Vida
topic Glutathione peroxidase
Glutathione reductase
Hepatitis in rats
.
Faculdade de Ciências da Vida
description Correlation between intensity of free radical processes estimated by biochemiluminesce parameters, content of lipoperoxidation products, and changes of glutathione peroxidase (GP, EC 1.11.1.9) and glutathione reductase (GR, EC 1.6.4.2) activities at rats liver injury, after 12, 36, 70, 96, 110, and 125 hours & tetrachloromethane administration have been investigated. The histological examination of the liver sections of rats showed that prominent hepatocytes with marked vacuolisation and inflammatory cells which were arranged around the necrotic tissue are more at 96 h after exposure to CCl4. Moreover maximum increase in GR and GP activities, 2.1 and 2.5 times, respectively, was observed at 96 h after exposure to CCl4, what coincided with the maximum of free radical oxidation processes. Using a combination of reverse transcription and real-time polymerase chain reaction, expression of the glutathione peroxidase and glutathione reductase genes (Gpx1 and Gsr) was analyzed by the determination of their respective mRNAs in the rat liver tissue under toxic hepatitis conditions. The analyses of Gpx1 and Gsr expression revealed that the transcript levels increased in 2.5- and 3.0-folds, respectively. Western blot analysis revealed that the amounts of hepatic Gpx1 and Gsr proteins increased considerably after CCl4 administration. It can be proposed that the overexpression of these enzymes could be a mechanism of enhancement of hepatocytes tolerance to oxidative stress.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-01T00:00:00Z
2013-01-01T00:00:00Z
2021-03-12T14:26:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.13/3166
url http://hdl.handle.net/10400.13/3166
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Iskusnykh, I. Y., Popova, T. N., Agarkov, A. A., Carvalho, M. Â.P., & Rjevskiy, S. G. (2013). Expression of glutathione peroxidase and glutathione reductase and level of free radical processes under toxic hepatitis in rats. Journal of Toxicology, 2013, 277-285. http://dx.doi.org/10.1155/2013/870628
10.1155/2013/870628
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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