Evidences for a new role of miR-214 in chondrogenesis
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/11329 |
Resumo: | miR-214 is known to play a role in mammalian skeletal development through inhibition of osteogenesis and stimulation of osteoclastogenesis, but data regarding other vertebrates, as well as a possible role in chondrogenesis, remain unknown. Here, we show that miR-214 expression is detected in bone and cartilage of zebrafish skeleton, and is downregulated during murine ATDC5 chondrocyte differentiation. Additionally, we observed a conservation of the transcriptional regulation of miR-214 primary transcript Dnm3os in vertebrates, being regulated by Ets1 in ATDC5 chondrogenic cells. Moreover, overexpression of miR-214 in vitro and in vivo mitigated chondrocyte differentiation probably by targeting activating transcription factor 4 (Atf4). Indeed, miR-214 overexpression in vivo hampered cranial cartilage formation of zebrafish and coincided with downregulation of atf4 and of the key chondrogenic players sox9 and col2a1. We show that miR-214 overexpression exerts a negative role in chondrogenesis by impacting on chondrocyte differentiation possibly through conserved mechanisms. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Evidences for a new role of miR-214 in chondrogenesisZebrafish Danio-RerioMatrix gla proteinCranial neural crestSkeletal developmentBone-formationOsteoblast differentiationGene-expressionOsteogenic DifferentiationGilthead seabreamCell lineagemiR-214 is known to play a role in mammalian skeletal development through inhibition of osteogenesis and stimulation of osteoclastogenesis, but data regarding other vertebrates, as well as a possible role in chondrogenesis, remain unknown. Here, we show that miR-214 expression is detected in bone and cartilage of zebrafish skeleton, and is downregulated during murine ATDC5 chondrocyte differentiation. Additionally, we observed a conservation of the transcriptional regulation of miR-214 primary transcript Dnm3os in vertebrates, being regulated by Ets1 in ATDC5 chondrogenic cells. Moreover, overexpression of miR-214 in vitro and in vivo mitigated chondrocyte differentiation probably by targeting activating transcription factor 4 (Atf4). Indeed, miR-214 overexpression in vivo hampered cranial cartilage formation of zebrafish and coincided with downregulation of atf4 and of the key chondrogenic players sox9 and col2a1. We show that miR-214 overexpression exerts a negative role in chondrogenesis by impacting on chondrocyte differentiation possibly through conserved mechanisms.Nature Publishing GroupSapientiaRoberto, Vania PalmaGavaia, PauloNunes, Maria JoaoRodrigues, ElsaM. Leonor CancelaTiago, Daniel2018-12-07T14:53:03Z2018-022018-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/11329eng2045-232210.1038/s41598-018-21735-winfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-29T10:50:57Zoai:sapientia.ualg.pt:10400.1/11329Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-29T10:50:57Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Evidences for a new role of miR-214 in chondrogenesis |
title |
Evidences for a new role of miR-214 in chondrogenesis |
spellingShingle |
Evidences for a new role of miR-214 in chondrogenesis Roberto, Vania Palma Zebrafish Danio-Rerio Matrix gla protein Cranial neural crest Skeletal development Bone-formation Osteoblast differentiation Gene-expression Osteogenic Differentiation Gilthead seabream Cell lineage |
title_short |
Evidences for a new role of miR-214 in chondrogenesis |
title_full |
Evidences for a new role of miR-214 in chondrogenesis |
title_fullStr |
Evidences for a new role of miR-214 in chondrogenesis |
title_full_unstemmed |
Evidences for a new role of miR-214 in chondrogenesis |
title_sort |
Evidences for a new role of miR-214 in chondrogenesis |
author |
Roberto, Vania Palma |
author_facet |
Roberto, Vania Palma Gavaia, Paulo Nunes, Maria Joao Rodrigues, Elsa M. Leonor Cancela Tiago, Daniel |
author_role |
author |
author2 |
Gavaia, Paulo Nunes, Maria Joao Rodrigues, Elsa M. Leonor Cancela Tiago, Daniel |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Roberto, Vania Palma Gavaia, Paulo Nunes, Maria Joao Rodrigues, Elsa M. Leonor Cancela Tiago, Daniel |
dc.subject.por.fl_str_mv |
Zebrafish Danio-Rerio Matrix gla protein Cranial neural crest Skeletal development Bone-formation Osteoblast differentiation Gene-expression Osteogenic Differentiation Gilthead seabream Cell lineage |
topic |
Zebrafish Danio-Rerio Matrix gla protein Cranial neural crest Skeletal development Bone-formation Osteoblast differentiation Gene-expression Osteogenic Differentiation Gilthead seabream Cell lineage |
description |
miR-214 is known to play a role in mammalian skeletal development through inhibition of osteogenesis and stimulation of osteoclastogenesis, but data regarding other vertebrates, as well as a possible role in chondrogenesis, remain unknown. Here, we show that miR-214 expression is detected in bone and cartilage of zebrafish skeleton, and is downregulated during murine ATDC5 chondrocyte differentiation. Additionally, we observed a conservation of the transcriptional regulation of miR-214 primary transcript Dnm3os in vertebrates, being regulated by Ets1 in ATDC5 chondrogenic cells. Moreover, overexpression of miR-214 in vitro and in vivo mitigated chondrocyte differentiation probably by targeting activating transcription factor 4 (Atf4). Indeed, miR-214 overexpression in vivo hampered cranial cartilage formation of zebrafish and coincided with downregulation of atf4 and of the key chondrogenic players sox9 and col2a1. We show that miR-214 overexpression exerts a negative role in chondrogenesis by impacting on chondrocyte differentiation possibly through conserved mechanisms. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-07T14:53:03Z 2018-02 2018-02-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/11329 |
url |
http://hdl.handle.net/10400.1/11329 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2045-2322 10.1038/s41598-018-21735-w |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817549837020692480 |