Production of nanofiber-based membranes for biomolecule recovery
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.6/3248 |
Resumo: | The demanding of ever-increasing quantities of highly purified biomolecules by bioindustries has triggered the development of new, more efficient, purification techniques. The application of membrane-based technologies has become very attractive in this field, for their high throughput capability, simplicity of operation and scale-up. In this thesis it is reported the production of a bi-layer membrane by electrospinning (ES), in which a support of poly ε-caprolactone nanofibers was coated with a polyethylene oxide/sodium alginate layer, and subsequently cross-linked with calcium chloride. The membranes were characterized by SEM, ATR-FTIR, contact angle measurements, and then were applied in the recovery process of a plasmid. The results obtained show that membranes retained the suspended solids while allowing the permeation of plasmid DNA, with high recovery yields and improved RNA retention. Moreover, they also showed a very low fouling tendency. |
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Production of nanofiber-based membranes for biomolecule recoveryProdução de membranas à base de nanofibras para a recuperação de biomoléculasMembranas de dupla faceElectrospinningThe demanding of ever-increasing quantities of highly purified biomolecules by bioindustries has triggered the development of new, more efficient, purification techniques. The application of membrane-based technologies has become very attractive in this field, for their high throughput capability, simplicity of operation and scale-up. In this thesis it is reported the production of a bi-layer membrane by electrospinning (ES), in which a support of poly ε-caprolactone nanofibers was coated with a polyethylene oxide/sodium alginate layer, and subsequently cross-linked with calcium chloride. The membranes were characterized by SEM, ATR-FTIR, contact angle measurements, and then were applied in the recovery process of a plasmid. The results obtained show that membranes retained the suspended solids while allowing the permeation of plasmid DNA, with high recovery yields and improved RNA retention. Moreover, they also showed a very low fouling tendency.A crescente procura de elevadas quantidades de biomoléculas altamente purificadas despoletou o desenvolvimento de novas, e mais eficientes, técnicas de purificação. A aplicação da tecnologia de membranas de microfiltração tem-se tornado muito atrativa nesta área, devido ao seu elevado rendimento, simplicidade de operação e de escalonamento. Na presente tese é apresentada a produção de uma membrana de dupla camada por electrospinning (ES), na qual um suporte de nanofibras de poli ε-caprolactona é revestido por uma camada de óxido polietileno/alginato de sódio, e subsequentemente reticulada em cloreto de cálcio. As membranas foram posteriormente caracterizadas por SEM, ATR-FTIR, determinação do ângulo de contacto. Posteriormente, as membranas foram usadas na recuperação de plasmídeo. Os resultados obtidos demonstraram que as membranas retêm os sólidos suspensos, permitindo a passagem de ADN plasmídeo, com elevados rendimentos de recuperação e melhorias na retenção de ARN. A membrana revela baixa tendência para a colmatação.Correia, Ilídio Joaquim SobreirauBibliorumAntunes, Bernardo Paiva2015-04-13T13:07:23Z2013-0620132013-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.6/3248porinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-15T09:39:36Zoai:ubibliorum.ubi.pt:10400.6/3248Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:44:43.399284Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Production of nanofiber-based membranes for biomolecule recovery Produção de membranas à base de nanofibras para a recuperação de biomoléculas |
title |
Production of nanofiber-based membranes for biomolecule recovery |
spellingShingle |
Production of nanofiber-based membranes for biomolecule recovery Antunes, Bernardo Paiva Membranas de dupla face Electrospinning |
title_short |
Production of nanofiber-based membranes for biomolecule recovery |
title_full |
Production of nanofiber-based membranes for biomolecule recovery |
title_fullStr |
Production of nanofiber-based membranes for biomolecule recovery |
title_full_unstemmed |
Production of nanofiber-based membranes for biomolecule recovery |
title_sort |
Production of nanofiber-based membranes for biomolecule recovery |
author |
Antunes, Bernardo Paiva |
author_facet |
Antunes, Bernardo Paiva |
author_role |
author |
dc.contributor.none.fl_str_mv |
Correia, Ilídio Joaquim Sobreira uBibliorum |
dc.contributor.author.fl_str_mv |
Antunes, Bernardo Paiva |
dc.subject.por.fl_str_mv |
Membranas de dupla face Electrospinning |
topic |
Membranas de dupla face Electrospinning |
description |
The demanding of ever-increasing quantities of highly purified biomolecules by bioindustries has triggered the development of new, more efficient, purification techniques. The application of membrane-based technologies has become very attractive in this field, for their high throughput capability, simplicity of operation and scale-up. In this thesis it is reported the production of a bi-layer membrane by electrospinning (ES), in which a support of poly ε-caprolactone nanofibers was coated with a polyethylene oxide/sodium alginate layer, and subsequently cross-linked with calcium chloride. The membranes were characterized by SEM, ATR-FTIR, contact angle measurements, and then were applied in the recovery process of a plasmid. The results obtained show that membranes retained the suspended solids while allowing the permeation of plasmid DNA, with high recovery yields and improved RNA retention. Moreover, they also showed a very low fouling tendency. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06 2013 2013-06-01T00:00:00Z 2015-04-13T13:07:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.6/3248 |
url |
http://hdl.handle.net/10400.6/3248 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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