New Method for the Monitoring of Antidepressants in Oral Fluid Using Dried Spot Sampling
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.26/41466 https://doi.org/10.3390/ph14121284 |
Resumo: | The increase in the consumption of antidepressants is a public health problem worldwide, as these are a class of compounds widely used in the treatment of several illnesses, such as depression and anxiety. This work aimed to develop and optimize a method for the quantification of a number of antidepressants and their metabolites (fluoxetine, venlafaxine, O-desmethylvenlafaxine, citalopram, sertraline, and paroxetine) in 100 μL of oral fluid using the dried saliva spots (DSS) sampling approach and gas chromatography coupled with tandem mass spectrometry (GC–MS/MS). The method was validated, presenting linearity within the studied range, with detection and quantification limits ranging between 10 and 100 ng/mL, and coefficients of determination (R2) of at least 0.99 for all analytes. Recoveries were between approximately 13 and 46%. The analysis of precision and accuracy presented acceptable coefficients of variation and relative errors, considering the criteria usually accepted in the validation of bioanalytical procedures. The method herein described is the first to be reported using DSS for the extraction of antidepressants, proving to be a sensitive, simple, and fast alternative to conventional techniques, and capable of being routinely applied in clinical and forensic toxicology scenarios |
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New Method for the Monitoring of Antidepressants in Oral Fluid Using Dried Spot SamplingAntidepressantBiological sampleDried saliva spotsGC–MS/MDrug monitorinThe increase in the consumption of antidepressants is a public health problem worldwide, as these are a class of compounds widely used in the treatment of several illnesses, such as depression and anxiety. This work aimed to develop and optimize a method for the quantification of a number of antidepressants and their metabolites (fluoxetine, venlafaxine, O-desmethylvenlafaxine, citalopram, sertraline, and paroxetine) in 100 μL of oral fluid using the dried saliva spots (DSS) sampling approach and gas chromatography coupled with tandem mass spectrometry (GC–MS/MS). The method was validated, presenting linearity within the studied range, with detection and quantification limits ranging between 10 and 100 ng/mL, and coefficients of determination (R2) of at least 0.99 for all analytes. Recoveries were between approximately 13 and 46%. The analysis of precision and accuracy presented acceptable coefficients of variation and relative errors, considering the criteria usually accepted in the validation of bioanalytical procedures. The method herein described is the first to be reported using DSS for the extraction of antidepressants, proving to be a sensitive, simple, and fast alternative to conventional techniques, and capable of being routinely applied in clinical and forensic toxicology scenariosinfo:eu-repo/semantics/publishedVersionMDPI2022-07-26T16:00:19Z2022-07-262021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10400.26/41466http://hdl.handle.net/10400.26/41466https://doi.org/10.3390/ph14121284engSoares S, Rosado T, Barroso M, Gallardo E. New Method for the Monitoring of Antidepressants in Oral Fluid Using Dried Spot Sampling. Pharmaceuticals. 2021; 14(12):12841424-8247https://www.mdpi.com/1424-8247/14/12/1284http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessSoares, SofiaRosado, TiagoBarroso, MárioGallardo, Eugeniareponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-03T04:22:03Zoai:comum.rcaap.pt:10400.26/41466Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:07:43.636940Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
New Method for the Monitoring of Antidepressants in Oral Fluid Using Dried Spot Sampling |
title |
New Method for the Monitoring of Antidepressants in Oral Fluid Using Dried Spot Sampling |
spellingShingle |
New Method for the Monitoring of Antidepressants in Oral Fluid Using Dried Spot Sampling Soares, Sofia Antidepressant Biological sample Dried saliva spots GC–MS/M Drug monitorin |
title_short |
New Method for the Monitoring of Antidepressants in Oral Fluid Using Dried Spot Sampling |
title_full |
New Method for the Monitoring of Antidepressants in Oral Fluid Using Dried Spot Sampling |
title_fullStr |
New Method for the Monitoring of Antidepressants in Oral Fluid Using Dried Spot Sampling |
title_full_unstemmed |
New Method for the Monitoring of Antidepressants in Oral Fluid Using Dried Spot Sampling |
title_sort |
New Method for the Monitoring of Antidepressants in Oral Fluid Using Dried Spot Sampling |
author |
Soares, Sofia |
author_facet |
Soares, Sofia Rosado, Tiago Barroso, Mário Gallardo, Eugenia |
author_role |
author |
author2 |
Rosado, Tiago Barroso, Mário Gallardo, Eugenia |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Soares, Sofia Rosado, Tiago Barroso, Mário Gallardo, Eugenia |
dc.subject.por.fl_str_mv |
Antidepressant Biological sample Dried saliva spots GC–MS/M Drug monitorin |
topic |
Antidepressant Biological sample Dried saliva spots GC–MS/M Drug monitorin |
description |
The increase in the consumption of antidepressants is a public health problem worldwide, as these are a class of compounds widely used in the treatment of several illnesses, such as depression and anxiety. This work aimed to develop and optimize a method for the quantification of a number of antidepressants and their metabolites (fluoxetine, venlafaxine, O-desmethylvenlafaxine, citalopram, sertraline, and paroxetine) in 100 μL of oral fluid using the dried saliva spots (DSS) sampling approach and gas chromatography coupled with tandem mass spectrometry (GC–MS/MS). The method was validated, presenting linearity within the studied range, with detection and quantification limits ranging between 10 and 100 ng/mL, and coefficients of determination (R2) of at least 0.99 for all analytes. Recoveries were between approximately 13 and 46%. The analysis of precision and accuracy presented acceptable coefficients of variation and relative errors, considering the criteria usually accepted in the validation of bioanalytical procedures. The method herein described is the first to be reported using DSS for the extraction of antidepressants, proving to be a sensitive, simple, and fast alternative to conventional techniques, and capable of being routinely applied in clinical and forensic toxicology scenarios |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01T00:00:00Z 2022-07-26T16:00:19Z 2022-07-26 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.26/41466 http://hdl.handle.net/10400.26/41466 https://doi.org/10.3390/ph14121284 |
url |
http://hdl.handle.net/10400.26/41466 https://doi.org/10.3390/ph14121284 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Soares S, Rosado T, Barroso M, Gallardo E. New Method for the Monitoring of Antidepressants in Oral Fluid Using Dried Spot Sampling. Pharmaceuticals. 2021; 14(12):1284 1424-8247 https://www.mdpi.com/1424-8247/14/12/1284 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137165899202560 |