Sulfur reduction in acid rock drainage environments
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/37613 |
Resumo: | Microbiological suitability of acidophilic sulfur reduction for metal recovery was explored by enriching sulfur reducers from acidic sediments at low pH (from 2 to 5) with hydrogen, glycerol, methanol and acetate as electron donors at 30 °C. The highest levels of sulfide in the enrichments were detected at pH 3 with hydrogen and pH 4 with acetate. Cloning and sequencing of the 16S rRNA gene showed dominance of the deltaproteobacterial sulfur-reducing genus Desulfurella in all the enrichments and subsequently an acidophilic strain (TR1) was isolated. Strain TR1 grew at a broad range of pH (37) and temperature (2050 °C) and showed good metal tolerance (Pb2+, Zn2+, Cu2+, Ni2+), especially for Ni2+ and Pb2+, with maximal tolerated concentrations of 0.09 and 0.03 mM, respectively. Different sources of sulfur were tested in the enrichments, from which biosulfur showed fastest growth (doubling time of 1.9 days), followed by colloidal, chemical and sublimated sulfur (doubling times of 2.2, 2.5, and 3.6 days, respectively). Strain TR1?s physiological traits make it a good candidate to cope with low pH and high metal concentration in biotechnological processes for treatment of metal-laden acidic streams at low and moderately high temperature. |
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Sulfur reduction in acid rock drainage environmentsScience & TechnologyMicrobiological suitability of acidophilic sulfur reduction for metal recovery was explored by enriching sulfur reducers from acidic sediments at low pH (from 2 to 5) with hydrogen, glycerol, methanol and acetate as electron donors at 30 °C. The highest levels of sulfide in the enrichments were detected at pH 3 with hydrogen and pH 4 with acetate. Cloning and sequencing of the 16S rRNA gene showed dominance of the deltaproteobacterial sulfur-reducing genus Desulfurella in all the enrichments and subsequently an acidophilic strain (TR1) was isolated. Strain TR1 grew at a broad range of pH (37) and temperature (2050 °C) and showed good metal tolerance (Pb2+, Zn2+, Cu2+, Ni2+), especially for Ni2+ and Pb2+, with maximal tolerated concentrations of 0.09 and 0.03 mM, respectively. Different sources of sulfur were tested in the enrichments, from which biosulfur showed fastest growth (doubling time of 1.9 days), followed by colloidal, chemical and sublimated sulfur (doubling times of 2.2, 2.5, and 3.6 days, respectively). Strain TR1?s physiological traits make it a good candidate to cope with low pH and high metal concentration in biotechnological processes for treatment of metal-laden acidic streams at low and moderately high temperature.American Chemical SocietyUniversidade do MinhoFlorentino, Anna P.Weijma, JanStams, Alfons Johannes MariaSánchez-Andrea, Irene20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/37613engFlorentino, Anna P.; Weijma, Jan; Stams, A. J. M.; Sánchez-Andrea, Irene, Sulfur reduction in acid rock drainage environments. Environmental Science and Technology, 49(19), 11746-11755, 20150013-936X1520-585110.1021/acs.est.5b0334626356416http://pubs.acs.org/journal/esthaginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:44:59Zoai:repositorium.sdum.uminho.pt:1822/37613Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:42:47.267928Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Sulfur reduction in acid rock drainage environments |
title |
Sulfur reduction in acid rock drainage environments |
spellingShingle |
Sulfur reduction in acid rock drainage environments Florentino, Anna P. Science & Technology |
title_short |
Sulfur reduction in acid rock drainage environments |
title_full |
Sulfur reduction in acid rock drainage environments |
title_fullStr |
Sulfur reduction in acid rock drainage environments |
title_full_unstemmed |
Sulfur reduction in acid rock drainage environments |
title_sort |
Sulfur reduction in acid rock drainage environments |
author |
Florentino, Anna P. |
author_facet |
Florentino, Anna P. Weijma, Jan Stams, Alfons Johannes Maria Sánchez-Andrea, Irene |
author_role |
author |
author2 |
Weijma, Jan Stams, Alfons Johannes Maria Sánchez-Andrea, Irene |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Florentino, Anna P. Weijma, Jan Stams, Alfons Johannes Maria Sánchez-Andrea, Irene |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
Microbiological suitability of acidophilic sulfur reduction for metal recovery was explored by enriching sulfur reducers from acidic sediments at low pH (from 2 to 5) with hydrogen, glycerol, methanol and acetate as electron donors at 30 °C. The highest levels of sulfide in the enrichments were detected at pH 3 with hydrogen and pH 4 with acetate. Cloning and sequencing of the 16S rRNA gene showed dominance of the deltaproteobacterial sulfur-reducing genus Desulfurella in all the enrichments and subsequently an acidophilic strain (TR1) was isolated. Strain TR1 grew at a broad range of pH (37) and temperature (2050 °C) and showed good metal tolerance (Pb2+, Zn2+, Cu2+, Ni2+), especially for Ni2+ and Pb2+, with maximal tolerated concentrations of 0.09 and 0.03 mM, respectively. Different sources of sulfur were tested in the enrichments, from which biosulfur showed fastest growth (doubling time of 1.9 days), followed by colloidal, chemical and sublimated sulfur (doubling times of 2.2, 2.5, and 3.6 days, respectively). Strain TR1?s physiological traits make it a good candidate to cope with low pH and high metal concentration in biotechnological processes for treatment of metal-laden acidic streams at low and moderately high temperature. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/37613 |
url |
http://hdl.handle.net/1822/37613 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Florentino, Anna P.; Weijma, Jan; Stams, A. J. M.; Sánchez-Andrea, Irene, Sulfur reduction in acid rock drainage environments. Environmental Science and Technology, 49(19), 11746-11755, 2015 0013-936X 1520-5851 10.1021/acs.est.5b03346 26356416 http://pubs.acs.org/journal/esthag |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132982019096576 |