Production of polygalacturonases by aspergillus section Nigri strains in a fixed bed reactor
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/22846 |
Resumo: | Polygalacturonases (PG) are pectinolytic enzymes that have technological, functional and biological applications in food processing, fruit ripening and plant-fungus interactions, respectively. In the present, study a microtitre plate methodology was used for rapid screening of 61 isolates of fungi from Aspergillus section Nigri to assess production of endo- and exo-PG. Studies of scale-up were carried out in a fixed bed reactor operated under different parameters using the best producer strain immobilised in orange peels. Four experiments were conducted under the following conditions: the immobilised cells without aeration; immobilised cells with aeration; immobilised cells with aeration and added pectin; and free cells with aeration. The fermentation was performed for 168 h with removal of sample every 24 h. Aspergillus niger strain URM 5162 showed the highest PG production. The results obtained indicated that the maximum endo- and exo-PG activities (1.18 U·mL-1 and 4.11 U·mL-1, respectively) were obtained when the reactor was operating without aeration. The microtitre plate method is a simple way to screen fungal isolates for PG activity detection. The fixed bed reactor with orange peel support and using A. niger URM 5162 is a promising process for PG production at the industrial level. |
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Production of polygalacturonases by aspergillus section Nigri strains in a fixed bed reactorAspergillus nigerOrange peelImmobilization cellsPolygalacturonasesFixed bed reactorScience & TechnologyPolygalacturonases (PG) are pectinolytic enzymes that have technological, functional and biological applications in food processing, fruit ripening and plant-fungus interactions, respectively. In the present, study a microtitre plate methodology was used for rapid screening of 61 isolates of fungi from Aspergillus section Nigri to assess production of endo- and exo-PG. Studies of scale-up were carried out in a fixed bed reactor operated under different parameters using the best producer strain immobilised in orange peels. Four experiments were conducted under the following conditions: the immobilised cells without aeration; immobilised cells with aeration; immobilised cells with aeration and added pectin; and free cells with aeration. The fermentation was performed for 168 h with removal of sample every 24 h. Aspergillus niger strain URM 5162 showed the highest PG production. The results obtained indicated that the maximum endo- and exo-PG activities (1.18 U·mL-1 and 4.11 U·mL-1, respectively) were obtained when the reactor was operating without aeration. The microtitre plate method is a simple way to screen fungal isolates for PG activity detection. The fixed bed reactor with orange peel support and using A. niger URM 5162 is a promising process for PG production at the industrial level.M.H.C. Maciel thanks to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil) and Erasmus Mundus External Cooperation Window Lote 17.MDPIUniversidade do MinhoMaciel, Marília de Holanda CavalcantiOttoni, C. A.Santos, C.Lima, NelsonMoreira, KeilaMotta, Cristina Souza20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/22846eng1420-304910.3390/molecules1802166023358324http://www.mdpi.com/journal/moleculesinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:25:32Zoai:repositorium.sdum.uminho.pt:1822/22846Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:19:47.472065Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Production of polygalacturonases by aspergillus section Nigri strains in a fixed bed reactor |
title |
Production of polygalacturonases by aspergillus section Nigri strains in a fixed bed reactor |
spellingShingle |
Production of polygalacturonases by aspergillus section Nigri strains in a fixed bed reactor Maciel, Marília de Holanda Cavalcanti Aspergillus niger Orange peel Immobilization cells Polygalacturonases Fixed bed reactor Science & Technology |
title_short |
Production of polygalacturonases by aspergillus section Nigri strains in a fixed bed reactor |
title_full |
Production of polygalacturonases by aspergillus section Nigri strains in a fixed bed reactor |
title_fullStr |
Production of polygalacturonases by aspergillus section Nigri strains in a fixed bed reactor |
title_full_unstemmed |
Production of polygalacturonases by aspergillus section Nigri strains in a fixed bed reactor |
title_sort |
Production of polygalacturonases by aspergillus section Nigri strains in a fixed bed reactor |
author |
Maciel, Marília de Holanda Cavalcanti |
author_facet |
Maciel, Marília de Holanda Cavalcanti Ottoni, C. A. Santos, C. Lima, Nelson Moreira, Keila Motta, Cristina Souza |
author_role |
author |
author2 |
Ottoni, C. A. Santos, C. Lima, Nelson Moreira, Keila Motta, Cristina Souza |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Maciel, Marília de Holanda Cavalcanti Ottoni, C. A. Santos, C. Lima, Nelson Moreira, Keila Motta, Cristina Souza |
dc.subject.por.fl_str_mv |
Aspergillus niger Orange peel Immobilization cells Polygalacturonases Fixed bed reactor Science & Technology |
topic |
Aspergillus niger Orange peel Immobilization cells Polygalacturonases Fixed bed reactor Science & Technology |
description |
Polygalacturonases (PG) are pectinolytic enzymes that have technological, functional and biological applications in food processing, fruit ripening and plant-fungus interactions, respectively. In the present, study a microtitre plate methodology was used for rapid screening of 61 isolates of fungi from Aspergillus section Nigri to assess production of endo- and exo-PG. Studies of scale-up were carried out in a fixed bed reactor operated under different parameters using the best producer strain immobilised in orange peels. Four experiments were conducted under the following conditions: the immobilised cells without aeration; immobilised cells with aeration; immobilised cells with aeration and added pectin; and free cells with aeration. The fermentation was performed for 168 h with removal of sample every 24 h. Aspergillus niger strain URM 5162 showed the highest PG production. The results obtained indicated that the maximum endo- and exo-PG activities (1.18 U·mL-1 and 4.11 U·mL-1, respectively) were obtained when the reactor was operating without aeration. The microtitre plate method is a simple way to screen fungal isolates for PG activity detection. The fixed bed reactor with orange peel support and using A. niger URM 5162 is a promising process for PG production at the industrial level. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2013-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/22846 |
url |
http://hdl.handle.net/1822/22846 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1420-3049 10.3390/molecules18021660 23358324 http://www.mdpi.com/journal/molecules |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132658223022080 |