Partial coated stem cells with bioinspired silica as new generation of cellular hybrid materials
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/34213 |
Resumo: | The manipulation of cell surfaces in anchorage-dependent cells can help overcome difficulties presented during cell handling and application. Silica-based protective mechanisms existing in free-floating microorganisms may be used as inspiration for sustaining human cell survival in suspension. Gathering on this, herein human adipose-derived mesenchymal stem cells (hASCs) are partially coated with a hard silica layer that operates as a supporting platform for individual cells in suspension. Inspired by the organic templates involved in biosilicification, a novel chitosan (CHT) derivative displaying fully natural quaternary amine moieties is synthetized via a rapid, one-pot strategy. Silicification is promoted on individual hASCs surfaces via a two-step process: a priming step onto previously adhered cell with this CHT derivative, followed by a biocompatible sol–gel process. hASCs holding a silica backpack exhibit enhanced cell survival in suspension conditions and can spread and acquire a more adherent phenotype. This new protocol for cell-surface modification also provides a new generation of hybrid materials with functionalization of the silica backpack, which can be applied to different areas such as tissue engineering, biosensing, drug delivery, and targeted cell-based therapies. |
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Partial coated stem cells with bioinspired silica as new generation of cellular hybrid materialsAdvanced cell-materials interactionsCell partial coatingsCell-based materialsOrganic-inorganic hybrid compositesSol-gel processesThe manipulation of cell surfaces in anchorage-dependent cells can help overcome difficulties presented during cell handling and application. Silica-based protective mechanisms existing in free-floating microorganisms may be used as inspiration for sustaining human cell survival in suspension. Gathering on this, herein human adipose-derived mesenchymal stem cells (hASCs) are partially coated with a hard silica layer that operates as a supporting platform for individual cells in suspension. Inspired by the organic templates involved in biosilicification, a novel chitosan (CHT) derivative displaying fully natural quaternary amine moieties is synthetized via a rapid, one-pot strategy. Silicification is promoted on individual hASCs surfaces via a two-step process: a priming step onto previously adhered cell with this CHT derivative, followed by a biocompatible sol–gel process. hASCs holding a silica backpack exhibit enhanced cell survival in suspension conditions and can spread and acquire a more adherent phenotype. This new protocol for cell-surface modification also provides a new generation of hybrid materials with functionalization of the silica backpack, which can be applied to different areas such as tissue engineering, biosensing, drug delivery, and targeted cell-based therapies.Wiley-Blackwell2022-07-20T09:24:47Z2021-07-16T00:00:00Z2021-07-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/34213eng1616-301X10.1002/adfm.202009619Maciel, Marta M.Correia, Tiago R.Gaspar, Vítor M.Rodrigues, João M. M.Choi, Insung S.Mano, João F.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:06:04Zoai:ria.ua.pt:10773/34213Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:05:35.895715Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Partial coated stem cells with bioinspired silica as new generation of cellular hybrid materials |
title |
Partial coated stem cells with bioinspired silica as new generation of cellular hybrid materials |
spellingShingle |
Partial coated stem cells with bioinspired silica as new generation of cellular hybrid materials Maciel, Marta M. Advanced cell-materials interactions Cell partial coatings Cell-based materials Organic-inorganic hybrid composites Sol-gel processes |
title_short |
Partial coated stem cells with bioinspired silica as new generation of cellular hybrid materials |
title_full |
Partial coated stem cells with bioinspired silica as new generation of cellular hybrid materials |
title_fullStr |
Partial coated stem cells with bioinspired silica as new generation of cellular hybrid materials |
title_full_unstemmed |
Partial coated stem cells with bioinspired silica as new generation of cellular hybrid materials |
title_sort |
Partial coated stem cells with bioinspired silica as new generation of cellular hybrid materials |
author |
Maciel, Marta M. |
author_facet |
Maciel, Marta M. Correia, Tiago R. Gaspar, Vítor M. Rodrigues, João M. M. Choi, Insung S. Mano, João F. |
author_role |
author |
author2 |
Correia, Tiago R. Gaspar, Vítor M. Rodrigues, João M. M. Choi, Insung S. Mano, João F. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Maciel, Marta M. Correia, Tiago R. Gaspar, Vítor M. Rodrigues, João M. M. Choi, Insung S. Mano, João F. |
dc.subject.por.fl_str_mv |
Advanced cell-materials interactions Cell partial coatings Cell-based materials Organic-inorganic hybrid composites Sol-gel processes |
topic |
Advanced cell-materials interactions Cell partial coatings Cell-based materials Organic-inorganic hybrid composites Sol-gel processes |
description |
The manipulation of cell surfaces in anchorage-dependent cells can help overcome difficulties presented during cell handling and application. Silica-based protective mechanisms existing in free-floating microorganisms may be used as inspiration for sustaining human cell survival in suspension. Gathering on this, herein human adipose-derived mesenchymal stem cells (hASCs) are partially coated with a hard silica layer that operates as a supporting platform for individual cells in suspension. Inspired by the organic templates involved in biosilicification, a novel chitosan (CHT) derivative displaying fully natural quaternary amine moieties is synthetized via a rapid, one-pot strategy. Silicification is promoted on individual hASCs surfaces via a two-step process: a priming step onto previously adhered cell with this CHT derivative, followed by a biocompatible sol–gel process. hASCs holding a silica backpack exhibit enhanced cell survival in suspension conditions and can spread and acquire a more adherent phenotype. This new protocol for cell-surface modification also provides a new generation of hybrid materials with functionalization of the silica backpack, which can be applied to different areas such as tissue engineering, biosensing, drug delivery, and targeted cell-based therapies. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-16T00:00:00Z 2021-07-16 2022-07-20T09:24:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/34213 |
url |
http://hdl.handle.net/10773/34213 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1616-301X 10.1002/adfm.202009619 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137711054913536 |