Cholesterol affects African swine fever virus infection

• Autor(a) principal: Bernardes, C.
• Data de Publicação: 1998
• Outros Autores: António, A., Pedroso de Lima, Maria C., Valdeira, M. L.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• DOI: 10.1016/S0005-2760(98)00051-4
• Texto Completo: http://hdl.handle.net/10316/3906; https://doi.org/10.1016/S0005-2760(98)00051-4
• topic: African swine fever virus; Cholesterol; African swine fever virus penetration; African swine fever virus fusion; African swine fever virus entry

African swine fever virus (ASFV) enters cells by receptor mediated endocytosis and requires a fusion event between the viral envelope and the limiting membrane of the endosome at low pH. In order to investigate the role of cholesterol in the early stages of ASFV infection, we have studied the effect of the removal of cell and viral membrane cholesterol by cholesterol oxidase treatment of cells and virions, as well as the effect of some inhibitors of cholesterol synthesis on the infectious pathway. In addition, we have investigated viral infection in cholesterol-depleted Vero cells. Both cholesterol-depleted and cholesterol oxidase-treated Vero cells were unaltered in their ability to bind or internalize the virus, but were blocked in ASFV fusion and subsequent virus replication. Our results indicate that ASFV infection is affected by cholesterol in the target membrane.