Differentiating present-day from ancient bones by vibrational spectroscopy upon acetic acid treatment
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.26/47377 https://doi.org/10.1016/j.forsciint.2023.111690 |
Resumo: | Acetic acid treatment for an accurate differentiation between ancient and recent human bones was assessed using Raman and FTIR-ATR spectroscopies. Each set of skeletal samples was analysed by these techniques, prior and after chemical washing, in order to determine the variations in bone´s chemical composition and crystallinity. Bone samples were collected from several independent sources: recent bones burned under controlled experimental conditions or cremated, and archaeological (XVII century and Iron Age). The effect of acetic acid, expected to impact mostly on carbonates, was clearly evidenced in the spectra of all samples, particularly in FTIR-ATR, mainly through the bands typical of A- and B-carbonates. Furthermore, as seen for crematoria and archaeological samples, acetic acid was found to remove contaminants such as calcium hydroxide. Overall, acetic acid treatment can be an effective method for removing carbonates (exogenous but possibly also endogenous) and external contaminants from bone. However, these effects are dependent on the skeletal conditions (e.g. post-mortem interval and burning settings). In addition, this chemical washing was shown to be insufficient for an unequivocal discrimination between recent and archaeological skeletal remains. Based on the measured IR indexes, only cremated bones could be clearly distinguished. |
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Differentiating present-day from ancient bones by vibrational spectroscopy upon acetic acid treatmentFTIR-ATR spectroscopyRaman spectroscopyPost-mortem intervalControlled burningCremationCarbonate contentAcetic acid treatment for an accurate differentiation between ancient and recent human bones was assessed using Raman and FTIR-ATR spectroscopies. Each set of skeletal samples was analysed by these techniques, prior and after chemical washing, in order to determine the variations in bone´s chemical composition and crystallinity. Bone samples were collected from several independent sources: recent bones burned under controlled experimental conditions or cremated, and archaeological (XVII century and Iron Age). The effect of acetic acid, expected to impact mostly on carbonates, was clearly evidenced in the spectra of all samples, particularly in FTIR-ATR, mainly through the bands typical of A- and B-carbonates. Furthermore, as seen for crematoria and archaeological samples, acetic acid was found to remove contaminants such as calcium hydroxide. Overall, acetic acid treatment can be an effective method for removing carbonates (exogenous but possibly also endogenous) and external contaminants from bone. However, these effects are dependent on the skeletal conditions (e.g. post-mortem interval and burning settings). In addition, this chemical washing was shown to be insufficient for an unequivocal discrimination between recent and archaeological skeletal remains. Based on the measured IR indexes, only cremated bones could be clearly distinguished.info:eu-repo/semantics/publishedVersionElsevier2023-10-20T16:39:27Z2023-10-202023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10400.26/47377http://hdl.handle.net/10400.26/47377https://doi.org/10.1016/j.forsciint.2023.111690engBrandão, A. L. C., Batista de Carvalho, L. A. E., Gonçalves, D., Piga, G., Cunha, E., & Marques, M. P. M. (2023). Differentiating present-day from ancient bones by vibrational spectroscopy upon acetic acid treatment. Forensic science international, 347, 111690http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessBrandão, A.L.C.Batista de Carvalho, L.A.E.Gonçalves, D.Piga, G.Cunha, E.Marques, M.P.M.reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-03T04:22:39Zoai:comum.rcaap.pt:10400.26/47377Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:07:51.832680Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Differentiating present-day from ancient bones by vibrational spectroscopy upon acetic acid treatment |
title |
Differentiating present-day from ancient bones by vibrational spectroscopy upon acetic acid treatment |
spellingShingle |
Differentiating present-day from ancient bones by vibrational spectroscopy upon acetic acid treatment Brandão, A.L.C. FTIR-ATR spectroscopy Raman spectroscopy Post-mortem interval Controlled burning Cremation Carbonate content |
title_short |
Differentiating present-day from ancient bones by vibrational spectroscopy upon acetic acid treatment |
title_full |
Differentiating present-day from ancient bones by vibrational spectroscopy upon acetic acid treatment |
title_fullStr |
Differentiating present-day from ancient bones by vibrational spectroscopy upon acetic acid treatment |
title_full_unstemmed |
Differentiating present-day from ancient bones by vibrational spectroscopy upon acetic acid treatment |
title_sort |
Differentiating present-day from ancient bones by vibrational spectroscopy upon acetic acid treatment |
author |
Brandão, A.L.C. |
author_facet |
Brandão, A.L.C. Batista de Carvalho, L.A.E. Gonçalves, D. Piga, G. Cunha, E. Marques, M.P.M. |
author_role |
author |
author2 |
Batista de Carvalho, L.A.E. Gonçalves, D. Piga, G. Cunha, E. Marques, M.P.M. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Brandão, A.L.C. Batista de Carvalho, L.A.E. Gonçalves, D. Piga, G. Cunha, E. Marques, M.P.M. |
dc.subject.por.fl_str_mv |
FTIR-ATR spectroscopy Raman spectroscopy Post-mortem interval Controlled burning Cremation Carbonate content |
topic |
FTIR-ATR spectroscopy Raman spectroscopy Post-mortem interval Controlled burning Cremation Carbonate content |
description |
Acetic acid treatment for an accurate differentiation between ancient and recent human bones was assessed using Raman and FTIR-ATR spectroscopies. Each set of skeletal samples was analysed by these techniques, prior and after chemical washing, in order to determine the variations in bone´s chemical composition and crystallinity. Bone samples were collected from several independent sources: recent bones burned under controlled experimental conditions or cremated, and archaeological (XVII century and Iron Age). The effect of acetic acid, expected to impact mostly on carbonates, was clearly evidenced in the spectra of all samples, particularly in FTIR-ATR, mainly through the bands typical of A- and B-carbonates. Furthermore, as seen for crematoria and archaeological samples, acetic acid was found to remove contaminants such as calcium hydroxide. Overall, acetic acid treatment can be an effective method for removing carbonates (exogenous but possibly also endogenous) and external contaminants from bone. However, these effects are dependent on the skeletal conditions (e.g. post-mortem interval and burning settings). In addition, this chemical washing was shown to be insufficient for an unequivocal discrimination between recent and archaeological skeletal remains. Based on the measured IR indexes, only cremated bones could be clearly distinguished. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-10-20T16:39:27Z 2023-10-20 2023-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.26/47377 http://hdl.handle.net/10400.26/47377 https://doi.org/10.1016/j.forsciint.2023.111690 |
url |
http://hdl.handle.net/10400.26/47377 https://doi.org/10.1016/j.forsciint.2023.111690 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brandão, A. L. C., Batista de Carvalho, L. A. E., Gonçalves, D., Piga, G., Cunha, E., & Marques, M. P. M. (2023). Differentiating present-day from ancient bones by vibrational spectroscopy upon acetic acid treatment. Forensic science international, 347, 111690 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137167855845376 |