The influence of P. fluorescens cell morphology on the lytic performance and production of phage ϕIBB-PF7A
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/22364 |
Resumo: | This study aims at assessing the influence of Pseudomonas fluorescence cell morphology on the effectiveness and production of the lytic bacteriophage /IBBPF7A. P. fluorescens were cultured as rods or as elongated cells by varying the temperature and rotary agitation conditions. Cells presented rod shape when grown at temperatures up to 25C and also at 30C under static conditions, and elongated morphology only at 30C when cultures were grown under agitation. Elongated cells were 0.4 up to 27.9 lm longer than rod cells. Rod-shaped hosts were best infected by phages at 25C which resulted in an 82% cell density reduction. Phage infection of elongated cells was successful, and the cell density reductions achieved was statistically similar (P[0.05) to those obtained at the optimum growth temperature of P. fluorescens. Phage burst size varied with the cell growth conditions and was approximately 58 and 153 PFU per infected rod and elongated cells, grown at 160 rpm, at 25C (the optimal temperature) and 30C, respectively. Phage adsorption was faster to elongated cells, most likely due to the longer length of the host. The surface composition of rod and elongated cells is similar in terms of outer membrane proteins and lipopolysaccharide profiles. The results of this study suggest that the change of rod cells to an elongated morphology does not prevent cells from being attacked by phages and also does not impair the phage infection. |
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The influence of P. fluorescens cell morphology on the lytic performance and production of phage ϕIBB-PF7AScience & TechnologyThis study aims at assessing the influence of Pseudomonas fluorescence cell morphology on the effectiveness and production of the lytic bacteriophage /IBBPF7A. P. fluorescens were cultured as rods or as elongated cells by varying the temperature and rotary agitation conditions. Cells presented rod shape when grown at temperatures up to 25C and also at 30C under static conditions, and elongated morphology only at 30C when cultures were grown under agitation. Elongated cells were 0.4 up to 27.9 lm longer than rod cells. Rod-shaped hosts were best infected by phages at 25C which resulted in an 82% cell density reduction. Phage infection of elongated cells was successful, and the cell density reductions achieved was statistically similar (P[0.05) to those obtained at the optimum growth temperature of P. fluorescens. Phage burst size varied with the cell growth conditions and was approximately 58 and 153 PFU per infected rod and elongated cells, grown at 160 rpm, at 25C (the optimal temperature) and 30C, respectively. Phage adsorption was faster to elongated cells, most likely due to the longer length of the host. The surface composition of rod and elongated cells is similar in terms of outer membrane proteins and lipopolysaccharide profiles. The results of this study suggest that the change of rod cells to an elongated morphology does not prevent cells from being attacked by phages and also does not impair the phage infection.This work was supported by a grant (SFRH/BD/18485/2004) from the Portuguese Foundation for Science and Technology (FCT).Springer VerlagUniversidade do MinhoSillankorva, SannaPires, Diana Priscila PensoOliveira, Hugo Alexandre MendesNeubauer, P.Azeredo, Joana20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/22364eng0343-865110.1007/s00284-011-9987-021789608info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:07:00Zoai:repositorium.sdum.uminho.pt:1822/22364Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:57:50.353233Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The influence of P. fluorescens cell morphology on the lytic performance and production of phage ϕIBB-PF7A |
title |
The influence of P. fluorescens cell morphology on the lytic performance and production of phage ϕIBB-PF7A |
spellingShingle |
The influence of P. fluorescens cell morphology on the lytic performance and production of phage ϕIBB-PF7A Sillankorva, Sanna Science & Technology |
title_short |
The influence of P. fluorescens cell morphology on the lytic performance and production of phage ϕIBB-PF7A |
title_full |
The influence of P. fluorescens cell morphology on the lytic performance and production of phage ϕIBB-PF7A |
title_fullStr |
The influence of P. fluorescens cell morphology on the lytic performance and production of phage ϕIBB-PF7A |
title_full_unstemmed |
The influence of P. fluorescens cell morphology on the lytic performance and production of phage ϕIBB-PF7A |
title_sort |
The influence of P. fluorescens cell morphology on the lytic performance and production of phage ϕIBB-PF7A |
author |
Sillankorva, Sanna |
author_facet |
Sillankorva, Sanna Pires, Diana Priscila Penso Oliveira, Hugo Alexandre Mendes Neubauer, P. Azeredo, Joana |
author_role |
author |
author2 |
Pires, Diana Priscila Penso Oliveira, Hugo Alexandre Mendes Neubauer, P. Azeredo, Joana |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Sillankorva, Sanna Pires, Diana Priscila Penso Oliveira, Hugo Alexandre Mendes Neubauer, P. Azeredo, Joana |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
This study aims at assessing the influence of Pseudomonas fluorescence cell morphology on the effectiveness and production of the lytic bacteriophage /IBBPF7A. P. fluorescens were cultured as rods or as elongated cells by varying the temperature and rotary agitation conditions. Cells presented rod shape when grown at temperatures up to 25C and also at 30C under static conditions, and elongated morphology only at 30C when cultures were grown under agitation. Elongated cells were 0.4 up to 27.9 lm longer than rod cells. Rod-shaped hosts were best infected by phages at 25C which resulted in an 82% cell density reduction. Phage infection of elongated cells was successful, and the cell density reductions achieved was statistically similar (P[0.05) to those obtained at the optimum growth temperature of P. fluorescens. Phage burst size varied with the cell growth conditions and was approximately 58 and 153 PFU per infected rod and elongated cells, grown at 160 rpm, at 25C (the optimal temperature) and 30C, respectively. Phage adsorption was faster to elongated cells, most likely due to the longer length of the host. The surface composition of rod and elongated cells is similar in terms of outer membrane proteins and lipopolysaccharide profiles. The results of this study suggest that the change of rod cells to an elongated morphology does not prevent cells from being attacked by phages and also does not impair the phage infection. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 2011-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/22364 |
url |
http://hdl.handle.net/1822/22364 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0343-8651 10.1007/s00284-011-9987-0 21789608 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Verlag |
publisher.none.fl_str_mv |
Springer Verlag |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132367825141760 |