Correlation of polymorphism of AQP7 gene with the sperm cells quality traits of the polish holstein friesian bulls
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/17332 |
Resumo: | The purpose of this study was to evaluate whether single nucleotide substitutions within exon 4 of the quality of AQP7 gene may affect the sperm cells of Polish Holstein-Friesian bulls. The research material was biological material (semen and blood) collected from 60 Holstein-Friesian bulls of the same age. The nucleotide substitutions analyzed are located in exon 4 of the AQP7 gene and are responsible for missense mutations. Individual genotypes were determined using the PCR-RFLP method. Evaluation of sperm quality in terms of concentration, volume, motility, and sperm viability was performed using photometry, flow cytometry, and a computer assisted sperm analyzer. The above studies indicate that all four nucleotide substitutions analyzed in exon 4 of AQP7 gene affect the individual quality characteristics of the analyzed semen of Holstein-Friesian bulls. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Correlation of polymorphism of AQP7 gene with the sperm cells quality traits of the polish holstein friesian bullsAQP7 geneHolstein-Friesian bullsAQP7 polymorphismReproductionSperm cells qualityAquaporinsAquaglycero-porinsSingle nucleotide polymorphismsThe purpose of this study was to evaluate whether single nucleotide substitutions within exon 4 of the quality of AQP7 gene may affect the sperm cells of Polish Holstein-Friesian bulls. The research material was biological material (semen and blood) collected from 60 Holstein-Friesian bulls of the same age. The nucleotide substitutions analyzed are located in exon 4 of the AQP7 gene and are responsible for missense mutations. Individual genotypes were determined using the PCR-RFLP method. Evaluation of sperm quality in terms of concentration, volume, motility, and sperm viability was performed using photometry, flow cytometry, and a computer assisted sperm analyzer. The above studies indicate that all four nucleotide substitutions analyzed in exon 4 of AQP7 gene affect the individual quality characteristics of the analyzed semen of Holstein-Friesian bulls.ElsevierBiblioteca Digital do IPBKowalczyk, AlicjaKowalewska-Łuczak, IngaCzerniawska-Piątkowska, EwaCantalapiedra, Jesús JuanCerqueira, J.O.L.Araújo, José Pedro2018-01-19T10:00:00Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/17332engKowalczyk, Alicja; Kowalewska-Łuczak, Inga; Czerniawska-Piątkowska, Ewa; Cantalapiedra, Jesús Juan; Cerqueira, J.O.L.;Araújo, José Pedro. (2023). Correlation of polymorphism of AQP7 gene with the sperm cells quality traits of the polish holstein friesian bulls. Zoological Society of Pakistan. ISSN 00309923. 55:1, p. 1-60030-992310.17582/journal.pjz/20210901170933info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-07T01:18:13Zoai:bibliotecadigital.ipb.pt:10198/17332Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:07:49.536779Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Correlation of polymorphism of AQP7 gene with the sperm cells quality traits of the polish holstein friesian bulls |
title |
Correlation of polymorphism of AQP7 gene with the sperm cells quality traits of the polish holstein friesian bulls |
spellingShingle |
Correlation of polymorphism of AQP7 gene with the sperm cells quality traits of the polish holstein friesian bulls Kowalczyk, Alicja AQP7 gene Holstein-Friesian bulls AQP7 polymorphism Reproduction Sperm cells quality Aquaporins Aquaglycero-porins Single nucleotide polymorphisms |
title_short |
Correlation of polymorphism of AQP7 gene with the sperm cells quality traits of the polish holstein friesian bulls |
title_full |
Correlation of polymorphism of AQP7 gene with the sperm cells quality traits of the polish holstein friesian bulls |
title_fullStr |
Correlation of polymorphism of AQP7 gene with the sperm cells quality traits of the polish holstein friesian bulls |
title_full_unstemmed |
Correlation of polymorphism of AQP7 gene with the sperm cells quality traits of the polish holstein friesian bulls |
title_sort |
Correlation of polymorphism of AQP7 gene with the sperm cells quality traits of the polish holstein friesian bulls |
author |
Kowalczyk, Alicja |
author_facet |
Kowalczyk, Alicja Kowalewska-Łuczak, Inga Czerniawska-Piątkowska, Ewa Cantalapiedra, Jesús Juan Cerqueira, J.O.L. Araújo, José Pedro |
author_role |
author |
author2 |
Kowalewska-Łuczak, Inga Czerniawska-Piątkowska, Ewa Cantalapiedra, Jesús Juan Cerqueira, J.O.L. Araújo, José Pedro |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Kowalczyk, Alicja Kowalewska-Łuczak, Inga Czerniawska-Piątkowska, Ewa Cantalapiedra, Jesús Juan Cerqueira, J.O.L. Araújo, José Pedro |
dc.subject.por.fl_str_mv |
AQP7 gene Holstein-Friesian bulls AQP7 polymorphism Reproduction Sperm cells quality Aquaporins Aquaglycero-porins Single nucleotide polymorphisms |
topic |
AQP7 gene Holstein-Friesian bulls AQP7 polymorphism Reproduction Sperm cells quality Aquaporins Aquaglycero-porins Single nucleotide polymorphisms |
description |
The purpose of this study was to evaluate whether single nucleotide substitutions within exon 4 of the quality of AQP7 gene may affect the sperm cells of Polish Holstein-Friesian bulls. The research material was biological material (semen and blood) collected from 60 Holstein-Friesian bulls of the same age. The nucleotide substitutions analyzed are located in exon 4 of the AQP7 gene and are responsible for missense mutations. Individual genotypes were determined using the PCR-RFLP method. Evaluation of sperm quality in terms of concentration, volume, motility, and sperm viability was performed using photometry, flow cytometry, and a computer assisted sperm analyzer. The above studies indicate that all four nucleotide substitutions analyzed in exon 4 of AQP7 gene affect the individual quality characteristics of the analyzed semen of Holstein-Friesian bulls. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-19T10:00:00Z 2023 2023-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/17332 |
url |
http://hdl.handle.net/10198/17332 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Kowalczyk, Alicja; Kowalewska-Łuczak, Inga; Czerniawska-Piątkowska, Ewa; Cantalapiedra, Jesús Juan; Cerqueira, J.O.L.;Araújo, José Pedro. (2023). Correlation of polymorphism of AQP7 gene with the sperm cells quality traits of the polish holstein friesian bulls. Zoological Society of Pakistan. ISSN 00309923. 55:1, p. 1-6 0030-9923 10.17582/journal.pjz/20210901170933 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799135335503888384 |