Molecular and virulence characterization of Toxoplasma gondii strains isolated from humans in Portugal
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/5213 |
Resumo: | Toxoplasma gondii is an apicomplexan parasite responsible for toxoplasmosis which infects all warm-blooded vertebrates, including mammals and birds. The majority of studies conducted in Europe have revealed that more than 80 % of strains isolated from human infections belong to genotype II, whereas genotypes I and III are responsible for a small number of cases. Atypical and recombinant strains are generally associated with more severe infections. In Portugal, there is a lack of data concerning genetic diversity as the classical typing studies in humans have never been performed. We aimed to determine the Sag2 and microsatellite-based (TUB2, TgM-A, W35, B17, B18) genotypes of T. gondii isolated from humans in Portugal, as well as to study their virulence in mice. We analyzed 48 strains from congenital and acquired toxoplasmosis collected during the last two decades. Sag2-based genotyping of T. gondii was achieved in all 48 strains where 35 (73 %) were classified as type II and 13 (27 %) were type I. The multilocus PCR of five microsatellites allowed the classification of 10 strains (21 %) as recombinant strains that had been previously identified as type II or I by Sag2 typing. Seven out of the 48 strains, including three type I, three recombinant, and one type I, were virulent in mice. This study constitutes the first evidence of recombinant strains circulating in Portugal in humans from congenital infection, highlighting the need for a better evaluation of these strains as their phenotype is still barely understood. |
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Molecular and virulence characterization of Toxoplasma gondii strains isolated from humans in PortugalAnimalsFemaleGenetic VariationGenotypeHumansMiceMice, Inbred ICRMicrosatellite RepeatsPolymerase Chain ReactionToxoplasmaToxoplasmosisVirulenceCongenitalToxoplasma gondiiRecombinantStrainPortugalInfecções Sistémicas e ZoonosesToxoplasma gondii is an apicomplexan parasite responsible for toxoplasmosis which infects all warm-blooded vertebrates, including mammals and birds. The majority of studies conducted in Europe have revealed that more than 80 % of strains isolated from human infections belong to genotype II, whereas genotypes I and III are responsible for a small number of cases. Atypical and recombinant strains are generally associated with more severe infections. In Portugal, there is a lack of data concerning genetic diversity as the classical typing studies in humans have never been performed. We aimed to determine the Sag2 and microsatellite-based (TUB2, TgM-A, W35, B17, B18) genotypes of T. gondii isolated from humans in Portugal, as well as to study their virulence in mice. We analyzed 48 strains from congenital and acquired toxoplasmosis collected during the last two decades. Sag2-based genotyping of T. gondii was achieved in all 48 strains where 35 (73 %) were classified as type II and 13 (27 %) were type I. The multilocus PCR of five microsatellites allowed the classification of 10 strains (21 %) as recombinant strains that had been previously identified as type II or I by Sag2 typing. Seven out of the 48 strains, including three type I, three recombinant, and one type I, were virulent in mice. This study constitutes the first evidence of recombinant strains circulating in Portugal in humans from congenital infection, highlighting the need for a better evaluation of these strains as their phenotype is still barely understood.Anabela Vilares (SFRH/BD/75154/2010) was the recipient of a PhD fellowship supported by Fundação para a Ciência e Tecnologia.Springer VerlagRepositório Científico do Instituto Nacional de SaúdeVilares, AnabelaGargaté, Maria JoãoFerreira, IdalinaMartins, SusanaGomes, João Paulo2018-03-06T15:32:11Z2017-032017-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/5213engParasitol Res. 2017 Mar;116(3):979-985. doi: 10.1007/s00436-017-5374-5. Epub 2017 Jan 30.0932-0113 (print)10.1007/s00436-017-5374-5info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:40:44Zoai:repositorio.insa.pt:10400.18/5213Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:39:55.845611Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Molecular and virulence characterization of Toxoplasma gondii strains isolated from humans in Portugal |
title |
Molecular and virulence characterization of Toxoplasma gondii strains isolated from humans in Portugal |
spellingShingle |
Molecular and virulence characterization of Toxoplasma gondii strains isolated from humans in Portugal Vilares, Anabela Animals Female Genetic Variation Genotype Humans Mice Mice, Inbred ICR Microsatellite Repeats Polymerase Chain Reaction Toxoplasma Toxoplasmosis Virulence Congenital Toxoplasma gondii Recombinant Strain Portugal Infecções Sistémicas e Zoonoses |
title_short |
Molecular and virulence characterization of Toxoplasma gondii strains isolated from humans in Portugal |
title_full |
Molecular and virulence characterization of Toxoplasma gondii strains isolated from humans in Portugal |
title_fullStr |
Molecular and virulence characterization of Toxoplasma gondii strains isolated from humans in Portugal |
title_full_unstemmed |
Molecular and virulence characterization of Toxoplasma gondii strains isolated from humans in Portugal |
title_sort |
Molecular and virulence characterization of Toxoplasma gondii strains isolated from humans in Portugal |
author |
Vilares, Anabela |
author_facet |
Vilares, Anabela Gargaté, Maria João Ferreira, Idalina Martins, Susana Gomes, João Paulo |
author_role |
author |
author2 |
Gargaté, Maria João Ferreira, Idalina Martins, Susana Gomes, João Paulo |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Vilares, Anabela Gargaté, Maria João Ferreira, Idalina Martins, Susana Gomes, João Paulo |
dc.subject.por.fl_str_mv |
Animals Female Genetic Variation Genotype Humans Mice Mice, Inbred ICR Microsatellite Repeats Polymerase Chain Reaction Toxoplasma Toxoplasmosis Virulence Congenital Toxoplasma gondii Recombinant Strain Portugal Infecções Sistémicas e Zoonoses |
topic |
Animals Female Genetic Variation Genotype Humans Mice Mice, Inbred ICR Microsatellite Repeats Polymerase Chain Reaction Toxoplasma Toxoplasmosis Virulence Congenital Toxoplasma gondii Recombinant Strain Portugal Infecções Sistémicas e Zoonoses |
description |
Toxoplasma gondii is an apicomplexan parasite responsible for toxoplasmosis which infects all warm-blooded vertebrates, including mammals and birds. The majority of studies conducted in Europe have revealed that more than 80 % of strains isolated from human infections belong to genotype II, whereas genotypes I and III are responsible for a small number of cases. Atypical and recombinant strains are generally associated with more severe infections. In Portugal, there is a lack of data concerning genetic diversity as the classical typing studies in humans have never been performed. We aimed to determine the Sag2 and microsatellite-based (TUB2, TgM-A, W35, B17, B18) genotypes of T. gondii isolated from humans in Portugal, as well as to study their virulence in mice. We analyzed 48 strains from congenital and acquired toxoplasmosis collected during the last two decades. Sag2-based genotyping of T. gondii was achieved in all 48 strains where 35 (73 %) were classified as type II and 13 (27 %) were type I. The multilocus PCR of five microsatellites allowed the classification of 10 strains (21 %) as recombinant strains that had been previously identified as type II or I by Sag2 typing. Seven out of the 48 strains, including three type I, three recombinant, and one type I, were virulent in mice. This study constitutes the first evidence of recombinant strains circulating in Portugal in humans from congenital infection, highlighting the need for a better evaluation of these strains as their phenotype is still barely understood. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03 2017-03-01T00:00:00Z 2018-03-06T15:32:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/5213 |
url |
http://hdl.handle.net/10400.18/5213 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Parasitol Res. 2017 Mar;116(3):979-985. doi: 10.1007/s00436-017-5374-5. Epub 2017 Jan 30. 0932-0113 (print) 10.1007/s00436-017-5374-5 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Verlag |
publisher.none.fl_str_mv |
Springer Verlag |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132139630886912 |