Molecular detection of Rickettsia spp. in ticks and fleas collected from rescued hedgehogs (Erinaceus europaeus) in Portugal.

Detalhes bibliográficos
Autor(a) principal: Barradas, Patrícia F.
Data de Publicação: 2021
Outros Autores: Mesquita, João R., Mateus, Teresa L., Ferreira, Paula, Amorim, Irina, Gärtner, Fátima
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/20.500.11960/3382
Resumo: Hedgehogs (e.g., Erinaceus europaeus, E. roumanicus) are wild mammals that frequently are observed near residential areas. The aim of this study was to investigate ticks and feas collected from European hedgehogs in Portugal and to evaluate the prevalence of Rickettsia in those ectoparasites. Ticks and feas were identifed by morphological and molecular methods, and molecular detection by PCR and genotypic characterization of Rickettsia spp. was performed targeting ompB, ompA and gltA gene fragments. In total, 1892 ticks and 213 feas were collected from 33 rescued European hedgehogs captured in seven districts of the north and centre of Portugal. Two tick species were identifed – Rhipicephalus sanguineus accounted for 91% (n=1719) of the total ticks collected and 9% (n=173) were Ixodes hexagonus. All feas were identifed as Archaeopsylla erinacei. Regarding pathogen detection, Rickettsia massiliae DNA was found in 22 of the 212 tested Rh. sanguineus. None of the 48 I. hexagonus tested showed to be positive for rickettsiae. Rickettsia asembonensis DNA was identifed in 55 A. erinacei feas tested (n=117). These results show that European hedgehogs are exposed to R. massiliae transmitted by ticks and to R. asembonensis via feas suggesting that these mammals might be involved in the natural transmission cycle of these Rickettsia species. This study is the frst report of R. asembonensis in feas in Portugal.
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spelling Molecular detection of Rickettsia spp. in ticks and fleas collected from rescued hedgehogs (Erinaceus europaeus) in Portugal.Rickettsia asembonensisArchaeopsylla erinaceiRhipicephalus sanguineusHedgehog · PortugalHedgehogs (e.g., Erinaceus europaeus, E. roumanicus) are wild mammals that frequently are observed near residential areas. The aim of this study was to investigate ticks and feas collected from European hedgehogs in Portugal and to evaluate the prevalence of Rickettsia in those ectoparasites. Ticks and feas were identifed by morphological and molecular methods, and molecular detection by PCR and genotypic characterization of Rickettsia spp. was performed targeting ompB, ompA and gltA gene fragments. In total, 1892 ticks and 213 feas were collected from 33 rescued European hedgehogs captured in seven districts of the north and centre of Portugal. Two tick species were identifed – Rhipicephalus sanguineus accounted for 91% (n=1719) of the total ticks collected and 9% (n=173) were Ixodes hexagonus. All feas were identifed as Archaeopsylla erinacei. Regarding pathogen detection, Rickettsia massiliae DNA was found in 22 of the 212 tested Rh. sanguineus. None of the 48 I. hexagonus tested showed to be positive for rickettsiae. Rickettsia asembonensis DNA was identifed in 55 A. erinacei feas tested (n=117). These results show that European hedgehogs are exposed to R. massiliae transmitted by ticks and to R. asembonensis via feas suggesting that these mammals might be involved in the natural transmission cycle of these Rickettsia species. This study is the frst report of R. asembonensis in feas in Portugal.2023-06-14T14:02:38Z2021-03-02T00:00:00Z2021-03-022022-11-27T16:41:26Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.11960/3382por10.1007/s10493-021-00600-yBarradas, Patrícia F.Mesquita, João R.Mateus, Teresa L.Ferreira, PaulaAmorim, IrinaGärtner, Fátimainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-06-22T06:45:15Zoai:repositorio.ipvc.pt:20.500.11960/3382Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:00:34.744713Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Molecular detection of Rickettsia spp. in ticks and fleas collected from rescued hedgehogs (Erinaceus europaeus) in Portugal.
title Molecular detection of Rickettsia spp. in ticks and fleas collected from rescued hedgehogs (Erinaceus europaeus) in Portugal.
spellingShingle Molecular detection of Rickettsia spp. in ticks and fleas collected from rescued hedgehogs (Erinaceus europaeus) in Portugal.
Barradas, Patrícia F.
Rickettsia asembonensis
Archaeopsylla erinacei
Rhipicephalus sanguineus
Hedgehog · Portugal
title_short Molecular detection of Rickettsia spp. in ticks and fleas collected from rescued hedgehogs (Erinaceus europaeus) in Portugal.
title_full Molecular detection of Rickettsia spp. in ticks and fleas collected from rescued hedgehogs (Erinaceus europaeus) in Portugal.
title_fullStr Molecular detection of Rickettsia spp. in ticks and fleas collected from rescued hedgehogs (Erinaceus europaeus) in Portugal.
title_full_unstemmed Molecular detection of Rickettsia spp. in ticks and fleas collected from rescued hedgehogs (Erinaceus europaeus) in Portugal.
title_sort Molecular detection of Rickettsia spp. in ticks and fleas collected from rescued hedgehogs (Erinaceus europaeus) in Portugal.
author Barradas, Patrícia F.
author_facet Barradas, Patrícia F.
Mesquita, João R.
Mateus, Teresa L.
Ferreira, Paula
Amorim, Irina
Gärtner, Fátima
author_role author
author2 Mesquita, João R.
Mateus, Teresa L.
Ferreira, Paula
Amorim, Irina
Gärtner, Fátima
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Barradas, Patrícia F.
Mesquita, João R.
Mateus, Teresa L.
Ferreira, Paula
Amorim, Irina
Gärtner, Fátima
dc.subject.por.fl_str_mv Rickettsia asembonensis
Archaeopsylla erinacei
Rhipicephalus sanguineus
Hedgehog · Portugal
topic Rickettsia asembonensis
Archaeopsylla erinacei
Rhipicephalus sanguineus
Hedgehog · Portugal
description Hedgehogs (e.g., Erinaceus europaeus, E. roumanicus) are wild mammals that frequently are observed near residential areas. The aim of this study was to investigate ticks and feas collected from European hedgehogs in Portugal and to evaluate the prevalence of Rickettsia in those ectoparasites. Ticks and feas were identifed by morphological and molecular methods, and molecular detection by PCR and genotypic characterization of Rickettsia spp. was performed targeting ompB, ompA and gltA gene fragments. In total, 1892 ticks and 213 feas were collected from 33 rescued European hedgehogs captured in seven districts of the north and centre of Portugal. Two tick species were identifed – Rhipicephalus sanguineus accounted for 91% (n=1719) of the total ticks collected and 9% (n=173) were Ixodes hexagonus. All feas were identifed as Archaeopsylla erinacei. Regarding pathogen detection, Rickettsia massiliae DNA was found in 22 of the 212 tested Rh. sanguineus. None of the 48 I. hexagonus tested showed to be positive for rickettsiae. Rickettsia asembonensis DNA was identifed in 55 A. erinacei feas tested (n=117). These results show that European hedgehogs are exposed to R. massiliae transmitted by ticks and to R. asembonensis via feas suggesting that these mammals might be involved in the natural transmission cycle of these Rickettsia species. This study is the frst report of R. asembonensis in feas in Portugal.
publishDate 2021
dc.date.none.fl_str_mv 2021-03-02T00:00:00Z
2021-03-02
2022-11-27T16:41:26Z
2023-06-14T14:02:38Z
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dc.relation.none.fl_str_mv 10.1007/s10493-021-00600-y
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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