Influence of surface characteristics on the adhesion of Alcaligenes denitrificans to polymeric substrates
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/1384 |
Resumo: | The adhesion of Alcaligenes denitrificans to several polymeric material was investigated. As the nature of surfaces of the micro-organisms and the substrate materials is an important factor in the adhesion process, characteristics such as the electrokinetic potential and hydrophobicity were also determined and correlated with the capacity of bacterial cells to adhere to solid surfaces. The substrates used were high-density polyethylene (HDPE), polypropylene (PP), poly (vinyl chloride) (PVC), and poly (methyl methacrylate) (PMMA). The electrokinetic potential of the cells and the substrates was determined by measurements of electrophoretic mobility and the hydrophobicity was determined by contact angle measurements. All the substrates studied as well as the bacterial strain have a negative zeta potential, which means that adhesion is not mediated by electrostatic interactions. As far as hydrophobicity is concerned, PP is the most hydrophobic material, PMMA is the least hydrophobic, whereas HDPE and PVC present an intermediate behavior. As bacterial cells are hydrophilic, adhesion is favored to PP; therefore, this substrate material seems to be the one that promotes a stronger adhesion and the development of the most stable biofilm for use as a biomass carrier in denitrifying inverse fluidized bed reactors. This was confirmed by the results of adhesion tests. In this way, adhesion seems to be dominated by hydrophobic interactions. |
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Influence of surface characteristics on the adhesion of Alcaligenes denitrificans to polymeric substratesInitial adhesionPolymeric substratesHydrophobicityZeta potentialMicro-organism adhesionAcid-baseScience & TechnologyThe adhesion of Alcaligenes denitrificans to several polymeric material was investigated. As the nature of surfaces of the micro-organisms and the substrate materials is an important factor in the adhesion process, characteristics such as the electrokinetic potential and hydrophobicity were also determined and correlated with the capacity of bacterial cells to adhere to solid surfaces. The substrates used were high-density polyethylene (HDPE), polypropylene (PP), poly (vinyl chloride) (PVC), and poly (methyl methacrylate) (PMMA). The electrokinetic potential of the cells and the substrates was determined by measurements of electrophoretic mobility and the hydrophobicity was determined by contact angle measurements. All the substrates studied as well as the bacterial strain have a negative zeta potential, which means that adhesion is not mediated by electrostatic interactions. As far as hydrophobicity is concerned, PP is the most hydrophobic material, PMMA is the least hydrophobic, whereas HDPE and PVC present an intermediate behavior. As bacterial cells are hydrophilic, adhesion is favored to PP; therefore, this substrate material seems to be the one that promotes a stronger adhesion and the development of the most stable biofilm for use as a biomass carrier in denitrifying inverse fluidized bed reactors. This was confirmed by the results of adhesion tests. In this way, adhesion seems to be dominated by hydrophobic interactions.PRAXIS XXI - BD/9121/96 - Project 01/REGII/6/96.VSPUniversidade do MinhoTeixeira, P.Oliveira, Rosário19991999-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/1384eng"Journal of adhesion science and technology". ISSN 0169-4243. 13:11 (1999) 1287-1294.0169-424310.1163/156856199X00190info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:51:04Zoai:repositorium.sdum.uminho.pt:1822/1384Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:49:52.959811Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Influence of surface characteristics on the adhesion of Alcaligenes denitrificans to polymeric substrates |
title |
Influence of surface characteristics on the adhesion of Alcaligenes denitrificans to polymeric substrates |
spellingShingle |
Influence of surface characteristics on the adhesion of Alcaligenes denitrificans to polymeric substrates Teixeira, P. Initial adhesion Polymeric substrates Hydrophobicity Zeta potential Micro-organism adhesion Acid-base Science & Technology |
title_short |
Influence of surface characteristics on the adhesion of Alcaligenes denitrificans to polymeric substrates |
title_full |
Influence of surface characteristics on the adhesion of Alcaligenes denitrificans to polymeric substrates |
title_fullStr |
Influence of surface characteristics on the adhesion of Alcaligenes denitrificans to polymeric substrates |
title_full_unstemmed |
Influence of surface characteristics on the adhesion of Alcaligenes denitrificans to polymeric substrates |
title_sort |
Influence of surface characteristics on the adhesion of Alcaligenes denitrificans to polymeric substrates |
author |
Teixeira, P. |
author_facet |
Teixeira, P. Oliveira, Rosário |
author_role |
author |
author2 |
Oliveira, Rosário |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Teixeira, P. Oliveira, Rosário |
dc.subject.por.fl_str_mv |
Initial adhesion Polymeric substrates Hydrophobicity Zeta potential Micro-organism adhesion Acid-base Science & Technology |
topic |
Initial adhesion Polymeric substrates Hydrophobicity Zeta potential Micro-organism adhesion Acid-base Science & Technology |
description |
The adhesion of Alcaligenes denitrificans to several polymeric material was investigated. As the nature of surfaces of the micro-organisms and the substrate materials is an important factor in the adhesion process, characteristics such as the electrokinetic potential and hydrophobicity were also determined and correlated with the capacity of bacterial cells to adhere to solid surfaces. The substrates used were high-density polyethylene (HDPE), polypropylene (PP), poly (vinyl chloride) (PVC), and poly (methyl methacrylate) (PMMA). The electrokinetic potential of the cells and the substrates was determined by measurements of electrophoretic mobility and the hydrophobicity was determined by contact angle measurements. All the substrates studied as well as the bacterial strain have a negative zeta potential, which means that adhesion is not mediated by electrostatic interactions. As far as hydrophobicity is concerned, PP is the most hydrophobic material, PMMA is the least hydrophobic, whereas HDPE and PVC present an intermediate behavior. As bacterial cells are hydrophilic, adhesion is favored to PP; therefore, this substrate material seems to be the one that promotes a stronger adhesion and the development of the most stable biofilm for use as a biomass carrier in denitrifying inverse fluidized bed reactors. This was confirmed by the results of adhesion tests. In this way, adhesion seems to be dominated by hydrophobic interactions. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999 1999-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/1384 |
url |
http://hdl.handle.net/1822/1384 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Journal of adhesion science and technology". ISSN 0169-4243. 13:11 (1999) 1287-1294. 0169-4243 10.1163/156856199X00190 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
VSP |
publisher.none.fl_str_mv |
VSP |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133081880231936 |