Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/34163 |
Resumo: | The build-up and degradation of cytocompatible nanofilms in a controlled fashion have great potential in biomedical and nanomedicinal fields, including single-cell nanoencapsulation (SCNE). Herein, we report the fabrication of biodegradable films of cationic starch (c-ST) and anionic alginate (ALG) by electrostatically driven layer-by-layer (LbL) assembly technology and its application to the SCNE. The [c-ST/ALG] multilayer nanofilms, assembled either on individual Saccharomyces cerevisiae or on the 2D flat gold surface, degrade on demand, in a cytocompatible fashion, via treatment with α-amylase. Their degradation profiles are investigated, while systematically changing the α-amylase concentration, by several surface characterization techniques, including quartz crystal microbalance with dissipation monitoring (QCM-D) and ellipsometry. DNA incorporation in the LbL nanofilms and its controlled release, upon exposure of the nanofilms to an aqueous α-amylase solution, are demonstrated. The highly cytocompatible nature of the film-forming and -degrading conditions is assessed in the c-ST/ALG-shell formation and degradation of S. cerevisiae. We envisage that the cytocompatible, enzymatic degradation of c-ST-based nanofilms paves the way for developing advanced biomedical devices with programmed dissolution in vivo. |
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Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulationThe build-up and degradation of cytocompatible nanofilms in a controlled fashion have great potential in biomedical and nanomedicinal fields, including single-cell nanoencapsulation (SCNE). Herein, we report the fabrication of biodegradable films of cationic starch (c-ST) and anionic alginate (ALG) by electrostatically driven layer-by-layer (LbL) assembly technology and its application to the SCNE. The [c-ST/ALG] multilayer nanofilms, assembled either on individual Saccharomyces cerevisiae or on the 2D flat gold surface, degrade on demand, in a cytocompatible fashion, via treatment with α-amylase. Their degradation profiles are investigated, while systematically changing the α-amylase concentration, by several surface characterization techniques, including quartz crystal microbalance with dissipation monitoring (QCM-D) and ellipsometry. DNA incorporation in the LbL nanofilms and its controlled release, upon exposure of the nanofilms to an aqueous α-amylase solution, are demonstrated. The highly cytocompatible nature of the film-forming and -degrading conditions is assessed in the c-ST/ALG-shell formation and degradation of S. cerevisiae. We envisage that the cytocompatible, enzymatic degradation of c-ST-based nanofilms paves the way for developing advanced biomedical devices with programmed dissolution in vivo.Royal Society of Chemistry2022-07-18T11:08:46Z2020-06-14T00:00:00Z2020-06-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/34163eng1744-683X10.1039/D0SM00876AMoon, Hee ChulHan, SolBorges, JoãoPesqueira, TamagnoChoi, HyunwooHan, Sang YeongCho, HyeoncheolPark, Ji HunMano, João F.Choi, Insung S.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:05:59Zoai:ria.ua.pt:10773/34163Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:05:34.032547Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation |
title |
Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation |
spellingShingle |
Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation Moon, Hee Chul |
title_short |
Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation |
title_full |
Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation |
title_fullStr |
Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation |
title_full_unstemmed |
Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation |
title_sort |
Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation |
author |
Moon, Hee Chul |
author_facet |
Moon, Hee Chul Han, Sol Borges, João Pesqueira, Tamagno Choi, Hyunwoo Han, Sang Yeong Cho, Hyeoncheol Park, Ji Hun Mano, João F. Choi, Insung S. |
author_role |
author |
author2 |
Han, Sol Borges, João Pesqueira, Tamagno Choi, Hyunwoo Han, Sang Yeong Cho, Hyeoncheol Park, Ji Hun Mano, João F. Choi, Insung S. |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Moon, Hee Chul Han, Sol Borges, João Pesqueira, Tamagno Choi, Hyunwoo Han, Sang Yeong Cho, Hyeoncheol Park, Ji Hun Mano, João F. Choi, Insung S. |
description |
The build-up and degradation of cytocompatible nanofilms in a controlled fashion have great potential in biomedical and nanomedicinal fields, including single-cell nanoencapsulation (SCNE). Herein, we report the fabrication of biodegradable films of cationic starch (c-ST) and anionic alginate (ALG) by electrostatically driven layer-by-layer (LbL) assembly technology and its application to the SCNE. The [c-ST/ALG] multilayer nanofilms, assembled either on individual Saccharomyces cerevisiae or on the 2D flat gold surface, degrade on demand, in a cytocompatible fashion, via treatment with α-amylase. Their degradation profiles are investigated, while systematically changing the α-amylase concentration, by several surface characterization techniques, including quartz crystal microbalance with dissipation monitoring (QCM-D) and ellipsometry. DNA incorporation in the LbL nanofilms and its controlled release, upon exposure of the nanofilms to an aqueous α-amylase solution, are demonstrated. The highly cytocompatible nature of the film-forming and -degrading conditions is assessed in the c-ST/ALG-shell formation and degradation of S. cerevisiae. We envisage that the cytocompatible, enzymatic degradation of c-ST-based nanofilms paves the way for developing advanced biomedical devices with programmed dissolution in vivo. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-06-14T00:00:00Z 2020-06-14 2022-07-18T11:08:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/34163 |
url |
http://hdl.handle.net/10773/34163 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1744-683X 10.1039/D0SM00876A |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137710270578688 |