Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation

Detalhes bibliográficos
Autor(a) principal: Moon, Hee Chul
Data de Publicação: 2020
Outros Autores: Han, Sol, Borges, João, Pesqueira, Tamagno, Choi, Hyunwoo, Han, Sang Yeong, Cho, Hyeoncheol, Park, Ji Hun, Mano, João F., Choi, Insung S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/34163
Resumo: The build-up and degradation of cytocompatible nanofilms in a controlled fashion have great potential in biomedical and nanomedicinal fields, including single-cell nanoencapsulation (SCNE). Herein, we report the fabrication of biodegradable films of cationic starch (c-ST) and anionic alginate (ALG) by electrostatically driven layer-by-layer (LbL) assembly technology and its application to the SCNE. The [c-ST/ALG] multilayer nanofilms, assembled either on individual Saccharomyces cerevisiae or on the 2D flat gold surface, degrade on demand, in a cytocompatible fashion, via treatment with α-amylase. Their degradation profiles are investigated, while systematically changing the α-amylase concentration, by several surface characterization techniques, including quartz crystal microbalance with dissipation monitoring (QCM-D) and ellipsometry. DNA incorporation in the LbL nanofilms and its controlled release, upon exposure of the nanofilms to an aqueous α-amylase solution, are demonstrated. The highly cytocompatible nature of the film-forming and -degrading conditions is assessed in the c-ST/ALG-shell formation and degradation of S. cerevisiae. We envisage that the cytocompatible, enzymatic degradation of c-ST-based nanofilms paves the way for developing advanced biomedical devices with programmed dissolution in vivo.
id RCAP_7a8185933cf641d38e79be2d87c1a581
oai_identifier_str oai:ria.ua.pt:10773/34163
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulationThe build-up and degradation of cytocompatible nanofilms in a controlled fashion have great potential in biomedical and nanomedicinal fields, including single-cell nanoencapsulation (SCNE). Herein, we report the fabrication of biodegradable films of cationic starch (c-ST) and anionic alginate (ALG) by electrostatically driven layer-by-layer (LbL) assembly technology and its application to the SCNE. The [c-ST/ALG] multilayer nanofilms, assembled either on individual Saccharomyces cerevisiae or on the 2D flat gold surface, degrade on demand, in a cytocompatible fashion, via treatment with α-amylase. Their degradation profiles are investigated, while systematically changing the α-amylase concentration, by several surface characterization techniques, including quartz crystal microbalance with dissipation monitoring (QCM-D) and ellipsometry. DNA incorporation in the LbL nanofilms and its controlled release, upon exposure of the nanofilms to an aqueous α-amylase solution, are demonstrated. The highly cytocompatible nature of the film-forming and -degrading conditions is assessed in the c-ST/ALG-shell formation and degradation of S. cerevisiae. We envisage that the cytocompatible, enzymatic degradation of c-ST-based nanofilms paves the way for developing advanced biomedical devices with programmed dissolution in vivo.Royal Society of Chemistry2022-07-18T11:08:46Z2020-06-14T00:00:00Z2020-06-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/34163eng1744-683X10.1039/D0SM00876AMoon, Hee ChulHan, SolBorges, JoãoPesqueira, TamagnoChoi, HyunwooHan, Sang YeongCho, HyeoncheolPark, Ji HunMano, João F.Choi, Insung S.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:05:59Zoai:ria.ua.pt:10773/34163Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:05:34.032547Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation
title Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation
spellingShingle Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation
Moon, Hee Chul
title_short Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation
title_full Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation
title_fullStr Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation
title_full_unstemmed Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation
title_sort Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation
author Moon, Hee Chul
author_facet Moon, Hee Chul
Han, Sol
Borges, João
Pesqueira, Tamagno
Choi, Hyunwoo
Han, Sang Yeong
Cho, Hyeoncheol
Park, Ji Hun
Mano, João F.
Choi, Insung S.
author_role author
author2 Han, Sol
Borges, João
Pesqueira, Tamagno
Choi, Hyunwoo
Han, Sang Yeong
Cho, Hyeoncheol
Park, Ji Hun
Mano, João F.
Choi, Insung S.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Moon, Hee Chul
Han, Sol
Borges, João
Pesqueira, Tamagno
Choi, Hyunwoo
Han, Sang Yeong
Cho, Hyeoncheol
Park, Ji Hun
Mano, João F.
Choi, Insung S.
description The build-up and degradation of cytocompatible nanofilms in a controlled fashion have great potential in biomedical and nanomedicinal fields, including single-cell nanoencapsulation (SCNE). Herein, we report the fabrication of biodegradable films of cationic starch (c-ST) and anionic alginate (ALG) by electrostatically driven layer-by-layer (LbL) assembly technology and its application to the SCNE. The [c-ST/ALG] multilayer nanofilms, assembled either on individual Saccharomyces cerevisiae or on the 2D flat gold surface, degrade on demand, in a cytocompatible fashion, via treatment with α-amylase. Their degradation profiles are investigated, while systematically changing the α-amylase concentration, by several surface characterization techniques, including quartz crystal microbalance with dissipation monitoring (QCM-D) and ellipsometry. DNA incorporation in the LbL nanofilms and its controlled release, upon exposure of the nanofilms to an aqueous α-amylase solution, are demonstrated. The highly cytocompatible nature of the film-forming and -degrading conditions is assessed in the c-ST/ALG-shell formation and degradation of S. cerevisiae. We envisage that the cytocompatible, enzymatic degradation of c-ST-based nanofilms paves the way for developing advanced biomedical devices with programmed dissolution in vivo.
publishDate 2020
dc.date.none.fl_str_mv 2020-06-14T00:00:00Z
2020-06-14
2022-07-18T11:08:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/34163
url http://hdl.handle.net/10773/34163
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1744-683X
10.1039/D0SM00876A
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799137710270578688