Hyperosmotic stress induces metacaspase - and mitochondria - dependent apoptosis in Saccharomyces cerevisiae

Detalhes bibliográficos
Autor(a) principal: Silva, Rui D.
Data de Publicação: 2005
Outros Autores: Sotoca, Roberto, Johansson, Björn, Ludovico, Paula, Sansonetty, Filipe, Silva, Manuel T., Peinado, José M., Côrte-Real, Manuela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/4353
Resumo: Prova tipográfica (In Press)
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spelling Hyperosmotic stress induces metacaspase - and mitochondria - dependent apoptosis in Saccharomyces cerevisiaeProgrammed cell deathCytochrome cYeastUltrastructural changes during apoptosisScience & TechnologyProva tipográfica (In Press)During the last years, several reports described an apoptosis-like programmed cell death process in yeast in response to different environmental aggressions. Here, evidence is presented that hyperosmotic stress induces in Saccharomyces cerevisiae a cell death process accompanied by morphological and biochemical indicators of apoptotic programmed cell death, namely chromatin condensation along the nuclear envelope, mitochondrial swelling and reduction of cristae number, production of reactive oxygen species and DNA strand breaks, with maintenance of plasma membrane integrity. Disruption of AIF1 had no effect on cell survival, but lack of Yca1p drastically reduced metacaspase activation and decreased cell death indicating that this death process was associated to activation of this protease. Supporting the involvement of mitochondria and cytochrome c in caspase activation, the mutant strains cyc1Δ cyc7Δ and cyc3Δ, both lacking mature cytochrome c, displayed a decrease in caspase activation associated to increased cell survival when exposed to hyperosmotic stress. These findings indicate that hyperosmotic stress triggers S. cerevisiae into an apoptosis-like programmed cell death that is mediated by a caspase-dependent mitochondrial pathway partially dependent on cytochrome c.Blackwell PublishingUniversidade do MinhoSilva, Rui D.Sotoca, RobertoJohansson, BjörnLudovico, PaulaSansonetty, FilipeSilva, Manuel T.Peinado, José M.Côrte-Real, Manuela20052005-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/4353eng0950-382X10.1111/j.1365-2958.2005.04868.x16238630info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:10:26Zoai:repositorium.sdum.uminho.pt:1822/4353Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:02:01.729520Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Hyperosmotic stress induces metacaspase - and mitochondria - dependent apoptosis in Saccharomyces cerevisiae
title Hyperosmotic stress induces metacaspase - and mitochondria - dependent apoptosis in Saccharomyces cerevisiae
spellingShingle Hyperosmotic stress induces metacaspase - and mitochondria - dependent apoptosis in Saccharomyces cerevisiae
Silva, Rui D.
Programmed cell death
Cytochrome c
Yeast
Ultrastructural changes during apoptosis
Science & Technology
title_short Hyperosmotic stress induces metacaspase - and mitochondria - dependent apoptosis in Saccharomyces cerevisiae
title_full Hyperosmotic stress induces metacaspase - and mitochondria - dependent apoptosis in Saccharomyces cerevisiae
title_fullStr Hyperosmotic stress induces metacaspase - and mitochondria - dependent apoptosis in Saccharomyces cerevisiae
title_full_unstemmed Hyperosmotic stress induces metacaspase - and mitochondria - dependent apoptosis in Saccharomyces cerevisiae
title_sort Hyperosmotic stress induces metacaspase - and mitochondria - dependent apoptosis in Saccharomyces cerevisiae
author Silva, Rui D.
author_facet Silva, Rui D.
Sotoca, Roberto
Johansson, Björn
Ludovico, Paula
Sansonetty, Filipe
Silva, Manuel T.
Peinado, José M.
Côrte-Real, Manuela
author_role author
author2 Sotoca, Roberto
Johansson, Björn
Ludovico, Paula
Sansonetty, Filipe
Silva, Manuel T.
Peinado, José M.
Côrte-Real, Manuela
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Silva, Rui D.
Sotoca, Roberto
Johansson, Björn
Ludovico, Paula
Sansonetty, Filipe
Silva, Manuel T.
Peinado, José M.
Côrte-Real, Manuela
dc.subject.por.fl_str_mv Programmed cell death
Cytochrome c
Yeast
Ultrastructural changes during apoptosis
Science & Technology
topic Programmed cell death
Cytochrome c
Yeast
Ultrastructural changes during apoptosis
Science & Technology
description Prova tipográfica (In Press)
publishDate 2005
dc.date.none.fl_str_mv 2005
2005-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/4353
url http://hdl.handle.net/1822/4353
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0950-382X
10.1111/j.1365-2958.2005.04868.x
16238630
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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