Extracellular Vesicles in Diffuse Large B Cell Lymphoma
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/145917 |
Resumo: | Funding Information: R.M. is supported by Fundação para a Ciência e a Tecnologia (CEEC position, 2019–2025 investigator). This article is a result of the projects (iNOVA4Health—UIDB/04462/2020), supported by Lisboa Portugal Regional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work is also funded by FEDER funds through the COMPETE 2020 Programme and National Funds through FCT—Portuguese Foundation for Science and Technology under the projects number PTDC/BTM-TEC/30087/2017 and PTDC/BTM-TEC/30088/2017. Publisher Copyright: © 2022 by the authors. |
| id |
RCAP_7af63a64354e92e2bc888ebfd064a505 |
|---|---|
| oai_identifier_str |
oai:run.unl.pt:10362/145917 |
| network_acronym_str |
RCAP |
| network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository_id_str |
7160 |
| spelling |
Extracellular Vesicles in Diffuse Large B Cell LymphomaCharacterization and Diagnostic Potentialcancerdiagnosticdiffuse large B cell lymphomaextracellular vesiclesliquid biopsyplasmaCatalysisMolecular BiologySpectroscopyComputer Science ApplicationsPhysical and Theoretical ChemistryOrganic ChemistryInorganic ChemistrySDG 3 - Good Health and Well-beingFunding Information: R.M. is supported by Fundação para a Ciência e a Tecnologia (CEEC position, 2019–2025 investigator). This article is a result of the projects (iNOVA4Health—UIDB/04462/2020), supported by Lisboa Portugal Regional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work is also funded by FEDER funds through the COMPETE 2020 Programme and National Funds through FCT—Portuguese Foundation for Science and Technology under the projects number PTDC/BTM-TEC/30087/2017 and PTDC/BTM-TEC/30088/2017. Publisher Copyright: © 2022 by the authors.Diffuse large B cell lymphoma (DLBCL) is an aggressive B cell lymphoma characterized by a heterogeneous behavior and in need of more accurate biological characterization monitoring and prognostic tools. Extracellular vesicles are secreted by all cell types and are currently established to some extent as representatives of the cell of origin. The present study characterized and evaluated the diagnostic and prognostic potential of plasma extracellular vesicles (EVs) proteome in DLBCL by using state-of-the-art mass spectrometry. The EV proteome is strongly affected by DLBCL status, with multiple proteins uniquely identified in the plasma of DLBCL. A proof-of-concept classifier resulted in highly accurate classification with a sensitivity and specificity of 1 when tested on the holdout test data set. On the other hand, no proteins were identified to correlate with non-germinal center B-cell like (non-GCB) or GCB subtypes to a significant degree after correction for multiple testing. However, functional analysis suggested that antigen binding is regulated when comparing non-GCB and GCB. Survival analysis based on protein quantitative values and clinical parameters identified multiple EV proteins as significantly correlated to survival. In conclusion, the plasma extracellular vesicle proteome identifies DLBCL cancer patients from healthy donors and contains potential EV protein markers for prediction of survival.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNMatthiesen, RuneGameiro, PaulaHenriques, AndreiaBodo, CristianMoraes, Maria Carolina StranoCosta-Silva, BrunoCabeçadas, JoséGomes da Silva, MariaBeck, Hans ChristianCarvalho, Ana Sofia2022-11-30T22:12:39Z2022-112022-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/145917eng1661-6596PURE: 47875564https://doi.org/10.3390/ijms232113327info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T18:07:04Zoai:run.unl.pt:10362/145917Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T18:07:04Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Extracellular Vesicles in Diffuse Large B Cell Lymphoma Characterization and Diagnostic Potential |
| title |
Extracellular Vesicles in Diffuse Large B Cell Lymphoma |
| spellingShingle |
Extracellular Vesicles in Diffuse Large B Cell Lymphoma Matthiesen, Rune cancer diagnostic diffuse large B cell lymphoma extracellular vesicles liquid biopsy plasma Catalysis Molecular Biology Spectroscopy Computer Science Applications Physical and Theoretical Chemistry Organic Chemistry Inorganic Chemistry SDG 3 - Good Health and Well-being |
| title_short |
Extracellular Vesicles in Diffuse Large B Cell Lymphoma |
| title_full |
Extracellular Vesicles in Diffuse Large B Cell Lymphoma |
| title_fullStr |
Extracellular Vesicles in Diffuse Large B Cell Lymphoma |
| title_full_unstemmed |
Extracellular Vesicles in Diffuse Large B Cell Lymphoma |
| title_sort |
Extracellular Vesicles in Diffuse Large B Cell Lymphoma |
| author |
Matthiesen, Rune |
| author_facet |
Matthiesen, Rune Gameiro, Paula Henriques, Andreia Bodo, Cristian Moraes, Maria Carolina Strano Costa-Silva, Bruno Cabeçadas, José Gomes da Silva, Maria Beck, Hans Christian Carvalho, Ana Sofia |
| author_role |
author |
| author2 |
Gameiro, Paula Henriques, Andreia Bodo, Cristian Moraes, Maria Carolina Strano Costa-Silva, Bruno Cabeçadas, José Gomes da Silva, Maria Beck, Hans Christian Carvalho, Ana Sofia |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) RUN |
| dc.contributor.author.fl_str_mv |
Matthiesen, Rune Gameiro, Paula Henriques, Andreia Bodo, Cristian Moraes, Maria Carolina Strano Costa-Silva, Bruno Cabeçadas, José Gomes da Silva, Maria Beck, Hans Christian Carvalho, Ana Sofia |
| dc.subject.por.fl_str_mv |
cancer diagnostic diffuse large B cell lymphoma extracellular vesicles liquid biopsy plasma Catalysis Molecular Biology Spectroscopy Computer Science Applications Physical and Theoretical Chemistry Organic Chemistry Inorganic Chemistry SDG 3 - Good Health and Well-being |
| topic |
cancer diagnostic diffuse large B cell lymphoma extracellular vesicles liquid biopsy plasma Catalysis Molecular Biology Spectroscopy Computer Science Applications Physical and Theoretical Chemistry Organic Chemistry Inorganic Chemistry SDG 3 - Good Health and Well-being |
| description |
Funding Information: R.M. is supported by Fundação para a Ciência e a Tecnologia (CEEC position, 2019–2025 investigator). This article is a result of the projects (iNOVA4Health—UIDB/04462/2020), supported by Lisboa Portugal Regional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work is also funded by FEDER funds through the COMPETE 2020 Programme and National Funds through FCT—Portuguese Foundation for Science and Technology under the projects number PTDC/BTM-TEC/30087/2017 and PTDC/BTM-TEC/30088/2017. Publisher Copyright: © 2022 by the authors. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-11-30T22:12:39Z 2022-11 2022-11-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/145917 |
| url |
http://hdl.handle.net/10362/145917 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1661-6596 PURE: 47875564 https://doi.org/10.3390/ijms232113327 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
| _version_ |
1817545901108887552 |