Metabolic shift induced by synthetic co-cultivation promotes high yield of chain elongated acids from syngas
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/62596 |
Resumo: | Bio-catalytic processes for sustainable production of chemicals and fuels receive increased attention within the concept of circular economy. Strategies to improve these production processes include genetic engineering of bio-catalysts or process technological optimization. Alternatively, synthetic microbial co-cultures can be used to enhance production of chemicals of interest. It remains often unclear however how microbe to microbe interactions affect the overall production process and how this can be further exploited for application. In the present study we explored the microbial interaction in a synthetic co-culture of Clostridium autoethanogenum and Clostridium kluyveri, producing chain elongated products from carbon monoxide. Monocultures of C. autoethanogenum converted CO to acetate and traces of ethanol, while during co-cultivation with C. kluyveri, it shifted its metabolism significantly towards solventogenesis. In C. autoethanogenum, expression of the genes involved in the central carbon- and energy-metabolism remained unchanged during co-cultivation compared to monoculture condition. Therefore the shift in the metabolic flux of C. autoethanogenum appears to be regulated by thermodynamics, and results from the continuous removal of ethanol by C. kluyveri. This trait could be further exploited, driving the metabolism of C. autoethanogenum to solely ethanol formation during co-cultivation, resulting in a high yield of chain elongated products from CO-derived electrons. This research highlights the important role of thermodynamic interactions in (synthetic) mixed microbial communities and shows that this can be exploited to promote desired conversions. |
id |
RCAP_7c00e48ee772743ea47bb743fa5c0305 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/62596 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Metabolic shift induced by synthetic co-cultivation promotes high yield of chain elongated acids from syngasScience & TechnologyBio-catalytic processes for sustainable production of chemicals and fuels receive increased attention within the concept of circular economy. Strategies to improve these production processes include genetic engineering of bio-catalysts or process technological optimization. Alternatively, synthetic microbial co-cultures can be used to enhance production of chemicals of interest. It remains often unclear however how microbe to microbe interactions affect the overall production process and how this can be further exploited for application. In the present study we explored the microbial interaction in a synthetic co-culture of Clostridium autoethanogenum and Clostridium kluyveri, producing chain elongated products from carbon monoxide. Monocultures of C. autoethanogenum converted CO to acetate and traces of ethanol, while during co-cultivation with C. kluyveri, it shifted its metabolism significantly towards solventogenesis. In C. autoethanogenum, expression of the genes involved in the central carbon- and energy-metabolism remained unchanged during co-cultivation compared to monoculture condition. Therefore the shift in the metabolic flux of C. autoethanogenum appears to be regulated by thermodynamics, and results from the continuous removal of ethanol by C. kluyveri. This trait could be further exploited, driving the metabolism of C. autoethanogenum to solely ethanol formation during co-cultivation, resulting in a high yield of chain elongated products from CO-derived electrons. This research highlights the important role of thermodynamic interactions in (synthetic) mixed microbial communities and shows that this can be exploited to promote desired conversions.The research leading to these results has received funding from the Netherlands Ministry of Education, Culture and Science and from the Netherlands Science Foundation (NWO) under the Gravitation Grant nr. 024.002.002 and Programme ‘Closed Cycles’ with Project nr. ALWGK.2016.029.info:eu-repo/semantics/publishedVersionNature ResearchUniversidade do MinhoDiender, MartijnParera Olm, IvetteGelderloos, MartenKoehorst, Jasper J.Schaap, Peter J.Stams, Alfons Johannes MariaSousa, Diana Zita Machado20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/62596engDiender, Martijn; Parera Olm, Ivette; Gelderloos, Marten; Koehorst, Jasper J.; Schaap, Peter J.; Stams, A. J. M.; Sousa, Diana Z., Metabolic shift induced by synthetic co-cultivation promotes high yield of chain elongated acids from syngas. Scientific Reports, 9(18081), 20192045-232210.1038/s41598-019-54445-y31792266http://www.nature.com/srep/index.htmlinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:46:04Zoai:repositorium.sdum.uminho.pt:1822/62596Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:44:00.116302Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Metabolic shift induced by synthetic co-cultivation promotes high yield of chain elongated acids from syngas |
title |
Metabolic shift induced by synthetic co-cultivation promotes high yield of chain elongated acids from syngas |
spellingShingle |
Metabolic shift induced by synthetic co-cultivation promotes high yield of chain elongated acids from syngas Diender, Martijn Science & Technology |
title_short |
Metabolic shift induced by synthetic co-cultivation promotes high yield of chain elongated acids from syngas |
title_full |
Metabolic shift induced by synthetic co-cultivation promotes high yield of chain elongated acids from syngas |
title_fullStr |
Metabolic shift induced by synthetic co-cultivation promotes high yield of chain elongated acids from syngas |
title_full_unstemmed |
Metabolic shift induced by synthetic co-cultivation promotes high yield of chain elongated acids from syngas |
title_sort |
Metabolic shift induced by synthetic co-cultivation promotes high yield of chain elongated acids from syngas |
author |
Diender, Martijn |
author_facet |
Diender, Martijn Parera Olm, Ivette Gelderloos, Marten Koehorst, Jasper J. Schaap, Peter J. Stams, Alfons Johannes Maria Sousa, Diana Zita Machado |
author_role |
author |
author2 |
Parera Olm, Ivette Gelderloos, Marten Koehorst, Jasper J. Schaap, Peter J. Stams, Alfons Johannes Maria Sousa, Diana Zita Machado |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Diender, Martijn Parera Olm, Ivette Gelderloos, Marten Koehorst, Jasper J. Schaap, Peter J. Stams, Alfons Johannes Maria Sousa, Diana Zita Machado |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
Bio-catalytic processes for sustainable production of chemicals and fuels receive increased attention within the concept of circular economy. Strategies to improve these production processes include genetic engineering of bio-catalysts or process technological optimization. Alternatively, synthetic microbial co-cultures can be used to enhance production of chemicals of interest. It remains often unclear however how microbe to microbe interactions affect the overall production process and how this can be further exploited for application. In the present study we explored the microbial interaction in a synthetic co-culture of Clostridium autoethanogenum and Clostridium kluyveri, producing chain elongated products from carbon monoxide. Monocultures of C. autoethanogenum converted CO to acetate and traces of ethanol, while during co-cultivation with C. kluyveri, it shifted its metabolism significantly towards solventogenesis. In C. autoethanogenum, expression of the genes involved in the central carbon- and energy-metabolism remained unchanged during co-cultivation compared to monoculture condition. Therefore the shift in the metabolic flux of C. autoethanogenum appears to be regulated by thermodynamics, and results from the continuous removal of ethanol by C. kluyveri. This trait could be further exploited, driving the metabolism of C. autoethanogenum to solely ethanol formation during co-cultivation, resulting in a high yield of chain elongated products from CO-derived electrons. This research highlights the important role of thermodynamic interactions in (synthetic) mixed microbial communities and shows that this can be exploited to promote desired conversions. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 2019-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/62596 |
url |
http://hdl.handle.net/1822/62596 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Diender, Martijn; Parera Olm, Ivette; Gelderloos, Marten; Koehorst, Jasper J.; Schaap, Peter J.; Stams, A. J. M.; Sousa, Diana Z., Metabolic shift induced by synthetic co-cultivation promotes high yield of chain elongated acids from syngas. Scientific Reports, 9(18081), 2019 2045-2322 10.1038/s41598-019-54445-y 31792266 http://www.nature.com/srep/index.html |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Nature Research |
publisher.none.fl_str_mv |
Nature Research |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132999227277312 |