Screening the olive tree phyllosphere: Search and find potential antagonists against Pseudomonas savastanoi pv. savastanoi

Detalhes bibliográficos
Autor(a) principal: Mina, Diogo
Data de Publicação: 2020
Outros Autores: Pereira, Jose Alberto, Lino-Neto, T., Baptista, Paula
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/73134
Resumo: The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2020.02051/full#supplementary-material
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spelling Screening the olive tree phyllosphere: Search and find potential antagonists against Pseudomonas savastanoi pv. savastanoiOlea europaeaolive knotbiocontrolBacillus amyloliquefaciensantagonistic mechanismsCiências Naturais::Ciências BiológicasScience & TechnologyThe Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2020.02051/full#supplementary-materialOlive knot (OK) is a widespread bacterial disease, caused byPseudomonas savastanoipv.savastanoi(Pss), which currently has not effective control methods. The use of naturally occurring microbial antagonists, such as bacteria, as biocontrol agents could be a strategy to manage this disease. The objective of this work was to select bacteria from olive tree phyllosphere able to antagonizePssusingin vitroandin plantaexperiments. The elucidation of their modes of action and the potential relationship between antagonism and bacteria origin has been investigated, as well. To this end, 60 bacterial isolates obtained from the surface and inner tissues of different organs (leaves, twigs, and knots), from two olive cultivars of varying susceptibilities to OK, were screened for theirin vitroantagonistic effect againstPss. A total of 27 bacterial strains were able to significantly inhibitPssgrowth, being this effect linked to bacteria origin. Strains from OK-susceptible cultivar and colonizing the surface of plant tissues showed the strongest antagonistic potential. The antagonistic activity was potentially due to the production of volatile compounds, siderophores and lytic enzymes.Bacillus amyloliquefaciensP41 was the most effective antagonistic strain and their capacity to control OK disease was subsequently assayed usingin plantaexperiments. This strain significantly reduces OK disease severity (43.7%), knots weight (55.4%) and population size ofPss(26.8%), while increasing the shoot dry weight (55.0%) and root water content (39.6%) ofPss-infected olive plantlets. Bacterial isolates characterized in this study, in particularB. amyloliquefaciensP41, may be considered as promising biocontrol candidates for controlling OK disease.This work was funded by FEDER funds through COMPETE (Programa Operacional Factores de Competitividade), national funds through FCT (Fundacao para a Ciencia e a Tecnologia) and by Horizon 2020, the European Union's Framework Programme for Research and Innovation, within the project PRIMA/0002/2018 (INTOMED - Innovative tools to combat crop pests in the Mediterranean), and the Mountain Research Center - CIMO (UIDB/00690/2020 and UIDB/04046/2020).Frontiers MediaUniversidade do MinhoMina, DiogoPereira, Jose AlbertoLino-Neto, T.Baptista, Paula20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/73134engMina D, Pereira JA, Lino-Neto T and Baptista P (2020) Screening the Olive Tree Phyllosphere: Search and Find Potential Antagonists Against Pseudomonas savastanoi pv. savastanoi. Front. Microbiol. 11:2051. doi: 10.3389/fmicb.2020.020511664-302X10.3389/fmicb.2020.02051https://www.frontiersin.org/articles/10.3389/fmicb.2020.02051info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:27:11Zoai:repositorium.sdum.uminho.pt:1822/73134Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:21:42.748257Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Screening the olive tree phyllosphere: Search and find potential antagonists against Pseudomonas savastanoi pv. savastanoi
title Screening the olive tree phyllosphere: Search and find potential antagonists against Pseudomonas savastanoi pv. savastanoi
spellingShingle Screening the olive tree phyllosphere: Search and find potential antagonists against Pseudomonas savastanoi pv. savastanoi
Mina, Diogo
Olea europaea
olive knot
biocontrol
Bacillus amyloliquefaciens
antagonistic mechanisms
Ciências Naturais::Ciências Biológicas
Science & Technology
title_short Screening the olive tree phyllosphere: Search and find potential antagonists against Pseudomonas savastanoi pv. savastanoi
title_full Screening the olive tree phyllosphere: Search and find potential antagonists against Pseudomonas savastanoi pv. savastanoi
title_fullStr Screening the olive tree phyllosphere: Search and find potential antagonists against Pseudomonas savastanoi pv. savastanoi
title_full_unstemmed Screening the olive tree phyllosphere: Search and find potential antagonists against Pseudomonas savastanoi pv. savastanoi
title_sort Screening the olive tree phyllosphere: Search and find potential antagonists against Pseudomonas savastanoi pv. savastanoi
author Mina, Diogo
author_facet Mina, Diogo
Pereira, Jose Alberto
Lino-Neto, T.
Baptista, Paula
author_role author
author2 Pereira, Jose Alberto
Lino-Neto, T.
Baptista, Paula
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Mina, Diogo
Pereira, Jose Alberto
Lino-Neto, T.
Baptista, Paula
dc.subject.por.fl_str_mv Olea europaea
olive knot
biocontrol
Bacillus amyloliquefaciens
antagonistic mechanisms
Ciências Naturais::Ciências Biológicas
Science & Technology
topic Olea europaea
olive knot
biocontrol
Bacillus amyloliquefaciens
antagonistic mechanisms
Ciências Naturais::Ciências Biológicas
Science & Technology
description The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2020.02051/full#supplementary-material
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/73134
url http://hdl.handle.net/1822/73134
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Mina D, Pereira JA, Lino-Neto T and Baptista P (2020) Screening the Olive Tree Phyllosphere: Search and Find Potential Antagonists Against Pseudomonas savastanoi pv. savastanoi. Front. Microbiol. 11:2051. doi: 10.3389/fmicb.2020.02051
1664-302X
10.3389/fmicb.2020.02051
https://www.frontiersin.org/articles/10.3389/fmicb.2020.02051
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132684372410368

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