Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/7594 |
Resumo: | Microbial communities and their associated metabolic activity in marine sediments have a profound impact on global biogeochemical cycles. Their composition and structure are attributed to geochemical and physical factors, but finding direct correlations has remained a challenge. Here we show a significant statistical relationship between variation in geochemical composition and prokaryotic community structure within deep-sea sediments. We obtained comprehensive geochemical data from two gravity cores near the hydrothermal vent field Loki’s Castle at the Arctic Mid-Ocean Ridge, in the Norwegian- Greenland Sea. Geochemical properties in the rift valley sediments exhibited strong centimeter-scale stratigraphic variability. Microbial populations were profiled by pyrosequencing from 15 sediment horizons (59,364 16S rRNA gene tags), quantitatively assessed by qPCR, and phylogenetically analyzed. Although the same taxa were generally present in all samples, their relative abundances varied substantially among horizons and fluctuated between Bacteria- and Archaea-dominated communities. By independently summarizing covariance structures of the relative abundance data and geochemical data, using principal components analysis, we found a significant correlation between changes in geochemical composition and changes in community structure. Differences in organic carbon and mineralogy shaped the relative abundance of microbial taxa. We used correlations to build hypotheses about energy metabolisms, particularly of the Deep Sea Archaeal Group, specific Deltaproteobacteria, and sediment lineages of potentially anaerobic Marine Group I Archaea. We demonstrate that total prokaryotic community structure can be directly correlated to geochemistry within these sediments, thus enhancing our understanding of biogeochemical cycling and our ability to predict metabolisms of uncultured microbes in deep-sea sediments. |
id |
RCAP_7d4dd911cef5f01707e04bd93bf4e0e3 |
---|---|
oai_identifier_str |
oai:dspace.uevora.pt:10174/7594 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridgetaxonomic profilingultraslow-spreading ridgeamplicon sequencingMicrobial communities and their associated metabolic activity in marine sediments have a profound impact on global biogeochemical cycles. Their composition and structure are attributed to geochemical and physical factors, but finding direct correlations has remained a challenge. Here we show a significant statistical relationship between variation in geochemical composition and prokaryotic community structure within deep-sea sediments. We obtained comprehensive geochemical data from two gravity cores near the hydrothermal vent field Loki’s Castle at the Arctic Mid-Ocean Ridge, in the Norwegian- Greenland Sea. Geochemical properties in the rift valley sediments exhibited strong centimeter-scale stratigraphic variability. Microbial populations were profiled by pyrosequencing from 15 sediment horizons (59,364 16S rRNA gene tags), quantitatively assessed by qPCR, and phylogenetically analyzed. Although the same taxa were generally present in all samples, their relative abundances varied substantially among horizons and fluctuated between Bacteria- and Archaea-dominated communities. By independently summarizing covariance structures of the relative abundance data and geochemical data, using principal components analysis, we found a significant correlation between changes in geochemical composition and changes in community structure. Differences in organic carbon and mineralogy shaped the relative abundance of microbial taxa. We used correlations to build hypotheses about energy metabolisms, particularly of the Deep Sea Archaeal Group, specific Deltaproteobacteria, and sediment lineages of potentially anaerobic Marine Group I Archaea. We demonstrate that total prokaryotic community structure can be directly correlated to geochemistry within these sediments, thus enhancing our understanding of biogeochemical cycling and our ability to predict metabolisms of uncultured microbes in deep-sea sediments.PNAS Edition2013-01-22T11:08:25Z2013-01-222012-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/7594http://hdl.handle.net/10174/7594enghttp://www.pnas.org/cgi/doi/10.1073/pnas.1207574109GEOsteffen.jorgensen@bio.uib.nobjarte.hannisdal@geo.uib.noanders.lanzen@uni.notamara.baumberger@geo.uib.noKristin.Flesland@geo.uib.norfonseca@uevora.ptLise.Ovreas@bio.uib.noIda.Steen@bio.uib.noIngunn.Thorseth@geo.uib.noRolf.Pedersen@geo.uib.nochrista.schleper@univie.ac.at371Jorgensen, Steffen LethHannisdal, BjarteLanzén, AndersBaumberger, TamaraFlesland, KristinFonseca, RitaØvreås, LiseSteen, Ida H.Thorseth, Ingunn H.Pedersen, Rolf B.Schleper, Christainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:48:04Zoai:dspace.uevora.pt:10174/7594Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:02:08.170278Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge |
title |
Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge |
spellingShingle |
Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge Jorgensen, Steffen Leth taxonomic profiling ultraslow-spreading ridge amplicon sequencing |
title_short |
Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge |
title_full |
Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge |
title_fullStr |
Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge |
title_full_unstemmed |
Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge |
title_sort |
Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge |
author |
Jorgensen, Steffen Leth |
author_facet |
Jorgensen, Steffen Leth Hannisdal, Bjarte Lanzén, Anders Baumberger, Tamara Flesland, Kristin Fonseca, Rita Øvreås, Lise Steen, Ida H. Thorseth, Ingunn H. Pedersen, Rolf B. Schleper, Christa |
author_role |
author |
author2 |
Hannisdal, Bjarte Lanzén, Anders Baumberger, Tamara Flesland, Kristin Fonseca, Rita Øvreås, Lise Steen, Ida H. Thorseth, Ingunn H. Pedersen, Rolf B. Schleper, Christa |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Jorgensen, Steffen Leth Hannisdal, Bjarte Lanzén, Anders Baumberger, Tamara Flesland, Kristin Fonseca, Rita Øvreås, Lise Steen, Ida H. Thorseth, Ingunn H. Pedersen, Rolf B. Schleper, Christa |
dc.subject.por.fl_str_mv |
taxonomic profiling ultraslow-spreading ridge amplicon sequencing |
topic |
taxonomic profiling ultraslow-spreading ridge amplicon sequencing |
description |
Microbial communities and their associated metabolic activity in marine sediments have a profound impact on global biogeochemical cycles. Their composition and structure are attributed to geochemical and physical factors, but finding direct correlations has remained a challenge. Here we show a significant statistical relationship between variation in geochemical composition and prokaryotic community structure within deep-sea sediments. We obtained comprehensive geochemical data from two gravity cores near the hydrothermal vent field Loki’s Castle at the Arctic Mid-Ocean Ridge, in the Norwegian- Greenland Sea. Geochemical properties in the rift valley sediments exhibited strong centimeter-scale stratigraphic variability. Microbial populations were profiled by pyrosequencing from 15 sediment horizons (59,364 16S rRNA gene tags), quantitatively assessed by qPCR, and phylogenetically analyzed. Although the same taxa were generally present in all samples, their relative abundances varied substantially among horizons and fluctuated between Bacteria- and Archaea-dominated communities. By independently summarizing covariance structures of the relative abundance data and geochemical data, using principal components analysis, we found a significant correlation between changes in geochemical composition and changes in community structure. Differences in organic carbon and mineralogy shaped the relative abundance of microbial taxa. We used correlations to build hypotheses about energy metabolisms, particularly of the Deep Sea Archaeal Group, specific Deltaproteobacteria, and sediment lineages of potentially anaerobic Marine Group I Archaea. We demonstrate that total prokaryotic community structure can be directly correlated to geochemistry within these sediments, thus enhancing our understanding of biogeochemical cycling and our ability to predict metabolisms of uncultured microbes in deep-sea sediments. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10-01T00:00:00Z 2013-01-22T11:08:25Z 2013-01-22 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/7594 http://hdl.handle.net/10174/7594 |
url |
http://hdl.handle.net/10174/7594 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.pnas.org/cgi/doi/10.1073/pnas.1207574109 GEO steffen.jorgensen@bio.uib.no bjarte.hannisdal@geo.uib.no anders.lanzen@uni.no tamara.baumberger@geo.uib.no Kristin.Flesland@geo.uib.no rfonseca@uevora.pt Lise.Ovreas@bio.uib.no Ida.Steen@bio.uib.no Ingunn.Thorseth@geo.uib.no Rolf.Pedersen@geo.uib.no christa.schleper@univie.ac.at 371 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
PNAS Edition |
publisher.none.fl_str_mv |
PNAS Edition |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799136504984895488 |