Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge

Detalhes bibliográficos
Autor(a) principal: Jorgensen, Steffen Leth
Data de Publicação: 2012
Outros Autores: Hannisdal, Bjarte, Lanzén, Anders, Baumberger, Tamara, Flesland, Kristin, Fonseca, Rita, Øvreås, Lise, Steen, Ida H., Thorseth, Ingunn H., Pedersen, Rolf B., Schleper, Christa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/7594
Resumo: Microbial communities and their associated metabolic activity in marine sediments have a profound impact on global biogeochemical cycles. Their composition and structure are attributed to geochemical and physical factors, but finding direct correlations has remained a challenge. Here we show a significant statistical relationship between variation in geochemical composition and prokaryotic community structure within deep-sea sediments. We obtained comprehensive geochemical data from two gravity cores near the hydrothermal vent field Loki’s Castle at the Arctic Mid-Ocean Ridge, in the Norwegian- Greenland Sea. Geochemical properties in the rift valley sediments exhibited strong centimeter-scale stratigraphic variability. Microbial populations were profiled by pyrosequencing from 15 sediment horizons (59,364 16S rRNA gene tags), quantitatively assessed by qPCR, and phylogenetically analyzed. Although the same taxa were generally present in all samples, their relative abundances varied substantially among horizons and fluctuated between Bacteria- and Archaea-dominated communities. By independently summarizing covariance structures of the relative abundance data and geochemical data, using principal components analysis, we found a significant correlation between changes in geochemical composition and changes in community structure. Differences in organic carbon and mineralogy shaped the relative abundance of microbial taxa. We used correlations to build hypotheses about energy metabolisms, particularly of the Deep Sea Archaeal Group, specific Deltaproteobacteria, and sediment lineages of potentially anaerobic Marine Group I Archaea. We demonstrate that total prokaryotic community structure can be directly correlated to geochemistry within these sediments, thus enhancing our understanding of biogeochemical cycling and our ability to predict metabolisms of uncultured microbes in deep-sea sediments.
id RCAP_7d4dd911cef5f01707e04bd93bf4e0e3
oai_identifier_str oai:dspace.uevora.pt:10174/7594
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridgetaxonomic profilingultraslow-spreading ridgeamplicon sequencingMicrobial communities and their associated metabolic activity in marine sediments have a profound impact on global biogeochemical cycles. Their composition and structure are attributed to geochemical and physical factors, but finding direct correlations has remained a challenge. Here we show a significant statistical relationship between variation in geochemical composition and prokaryotic community structure within deep-sea sediments. We obtained comprehensive geochemical data from two gravity cores near the hydrothermal vent field Loki’s Castle at the Arctic Mid-Ocean Ridge, in the Norwegian- Greenland Sea. Geochemical properties in the rift valley sediments exhibited strong centimeter-scale stratigraphic variability. Microbial populations were profiled by pyrosequencing from 15 sediment horizons (59,364 16S rRNA gene tags), quantitatively assessed by qPCR, and phylogenetically analyzed. Although the same taxa were generally present in all samples, their relative abundances varied substantially among horizons and fluctuated between Bacteria- and Archaea-dominated communities. By independently summarizing covariance structures of the relative abundance data and geochemical data, using principal components analysis, we found a significant correlation between changes in geochemical composition and changes in community structure. Differences in organic carbon and mineralogy shaped the relative abundance of microbial taxa. We used correlations to build hypotheses about energy metabolisms, particularly of the Deep Sea Archaeal Group, specific Deltaproteobacteria, and sediment lineages of potentially anaerobic Marine Group I Archaea. We demonstrate that total prokaryotic community structure can be directly correlated to geochemistry within these sediments, thus enhancing our understanding of biogeochemical cycling and our ability to predict metabolisms of uncultured microbes in deep-sea sediments.PNAS Edition2013-01-22T11:08:25Z2013-01-222012-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/7594http://hdl.handle.net/10174/7594enghttp://www.pnas.org/cgi/doi/10.1073/pnas.1207574109GEOsteffen.jorgensen@bio.uib.nobjarte.hannisdal@geo.uib.noanders.lanzen@uni.notamara.baumberger@geo.uib.noKristin.Flesland@geo.uib.norfonseca@uevora.ptLise.Ovreas@bio.uib.noIda.Steen@bio.uib.noIngunn.Thorseth@geo.uib.noRolf.Pedersen@geo.uib.nochrista.schleper@univie.ac.at371Jorgensen, Steffen LethHannisdal, BjarteLanzén, AndersBaumberger, TamaraFlesland, KristinFonseca, RitaØvreås, LiseSteen, Ida H.Thorseth, Ingunn H.Pedersen, Rolf B.Schleper, Christainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:48:04Zoai:dspace.uevora.pt:10174/7594Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:02:08.170278Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge
title Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge
spellingShingle Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge
Jorgensen, Steffen Leth
taxonomic profiling
ultraslow-spreading ridge
amplicon sequencing
title_short Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge
title_full Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge
title_fullStr Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge
title_full_unstemmed Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge
title_sort Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge
author Jorgensen, Steffen Leth
author_facet Jorgensen, Steffen Leth
Hannisdal, Bjarte
Lanzén, Anders
Baumberger, Tamara
Flesland, Kristin
Fonseca, Rita
Øvreås, Lise
Steen, Ida H.
Thorseth, Ingunn H.
Pedersen, Rolf B.
Schleper, Christa
author_role author
author2 Hannisdal, Bjarte
Lanzén, Anders
Baumberger, Tamara
Flesland, Kristin
Fonseca, Rita
Øvreås, Lise
Steen, Ida H.
Thorseth, Ingunn H.
Pedersen, Rolf B.
Schleper, Christa
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Jorgensen, Steffen Leth
Hannisdal, Bjarte
Lanzén, Anders
Baumberger, Tamara
Flesland, Kristin
Fonseca, Rita
Øvreås, Lise
Steen, Ida H.
Thorseth, Ingunn H.
Pedersen, Rolf B.
Schleper, Christa
dc.subject.por.fl_str_mv taxonomic profiling
ultraslow-spreading ridge
amplicon sequencing
topic taxonomic profiling
ultraslow-spreading ridge
amplicon sequencing
description Microbial communities and their associated metabolic activity in marine sediments have a profound impact on global biogeochemical cycles. Their composition and structure are attributed to geochemical and physical factors, but finding direct correlations has remained a challenge. Here we show a significant statistical relationship between variation in geochemical composition and prokaryotic community structure within deep-sea sediments. We obtained comprehensive geochemical data from two gravity cores near the hydrothermal vent field Loki’s Castle at the Arctic Mid-Ocean Ridge, in the Norwegian- Greenland Sea. Geochemical properties in the rift valley sediments exhibited strong centimeter-scale stratigraphic variability. Microbial populations were profiled by pyrosequencing from 15 sediment horizons (59,364 16S rRNA gene tags), quantitatively assessed by qPCR, and phylogenetically analyzed. Although the same taxa were generally present in all samples, their relative abundances varied substantially among horizons and fluctuated between Bacteria- and Archaea-dominated communities. By independently summarizing covariance structures of the relative abundance data and geochemical data, using principal components analysis, we found a significant correlation between changes in geochemical composition and changes in community structure. Differences in organic carbon and mineralogy shaped the relative abundance of microbial taxa. We used correlations to build hypotheses about energy metabolisms, particularly of the Deep Sea Archaeal Group, specific Deltaproteobacteria, and sediment lineages of potentially anaerobic Marine Group I Archaea. We demonstrate that total prokaryotic community structure can be directly correlated to geochemistry within these sediments, thus enhancing our understanding of biogeochemical cycling and our ability to predict metabolisms of uncultured microbes in deep-sea sediments.
publishDate 2012
dc.date.none.fl_str_mv 2012-10-01T00:00:00Z
2013-01-22T11:08:25Z
2013-01-22
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/7594
http://hdl.handle.net/10174/7594
url http://hdl.handle.net/10174/7594
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://www.pnas.org/cgi/doi/10.1073/pnas.1207574109
GEO
steffen.jorgensen@bio.uib.no
bjarte.hannisdal@geo.uib.no
anders.lanzen@uni.no
tamara.baumberger@geo.uib.no
Kristin.Flesland@geo.uib.no
rfonseca@uevora.pt
Lise.Ovreas@bio.uib.no
Ida.Steen@bio.uib.no
Ingunn.Thorseth@geo.uib.no
Rolf.Pedersen@geo.uib.no
christa.schleper@univie.ac.at
371
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv PNAS Edition
publisher.none.fl_str_mv PNAS Edition
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799136504984895488