Validation of a method for cylindrospermopsin determination in vegetables: Application to real samples such as lettuce (lactuca sativa l.)
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/10216/120503 |
Resumo: | Reports on the occurrence of the cyanobacterial toxin cylindrospermopsin (CYN) have increased worldwide because of CYN toxic effects in humans and animals. If contaminated waters are used for plant irrigation, these could represent a possible CYN exposure route for humans. For the first time, a method employing solid phase extraction and quantification by ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) of CYN was optimized in vegetables matrices such as lettuce (Lactuca sativa). The validated method showed a linear range, from 5 to 500 ng CYN g−1 of fresh weight (f.w.), and detection and quantitation limits (LOD and LOQ) of 0.22 and 0.42 ng CYN g−1 f.w., respectively. The mean recoveries ranged between 85 and 104%, and the intermediate precision from 12.7 to 14.7%. The method showed to be robust for the three different variables tested. Moreover, it was successfully applied to quantify CYN in edible lettuce leaves exposed to CYN-contaminated water (10 µg L−1), showing that the tolerable daily intake (TDI) in the case of CYN could be exceeded in elderly high consumers. The validated method showed good results in terms of sensitivity, precision, accuracy, and robustness for CYN determination in leaf vegetables such as lettuce. More studies are needed in order to prevent the risks associated with the consumption of CYN-contaminated vegetables. © 2018 by the authors. Licensee MDPI, Basel, Switzerland. |
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Validation of a method for cylindrospermopsin determination in vegetables: Application to real samples such as lettuce (lactuca sativa l.)cylindrospermopsinbacterial toxincylindrospermopsinuracilanalytical parametersArticledrug determinationfreeze dryinglettucelimit of detectionlimit of quantitationlinearityliquid chromatography-mass spectrometrymeasurement accuracymeasurement precisionnonhumanplant leafrisk assessmentrobustnessruggednesssensitivity analysissolvent extractionultra performance liquid chromatographyvalidation processvegetableanalogs and derivativesanalysischemistryfood contaminationirrigation (agriculture)lettuceliquid chromatographyprocedurestandem mass spectrometryvegetableAgricultural IrrigationBacterial ToxinsChromatography, LiquidFood ContaminationLettuceLimit of DetectionPlant LeavesTandem Mass SpectrometryUracilVegetablesReports on the occurrence of the cyanobacterial toxin cylindrospermopsin (CYN) have increased worldwide because of CYN toxic effects in humans and animals. If contaminated waters are used for plant irrigation, these could represent a possible CYN exposure route for humans. For the first time, a method employing solid phase extraction and quantification by ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) of CYN was optimized in vegetables matrices such as lettuce (Lactuca sativa). The validated method showed a linear range, from 5 to 500 ng CYN g−1 of fresh weight (f.w.), and detection and quantitation limits (LOD and LOQ) of 0.22 and 0.42 ng CYN g−1 f.w., respectively. The mean recoveries ranged between 85 and 104%, and the intermediate precision from 12.7 to 14.7%. The method showed to be robust for the three different variables tested. Moreover, it was successfully applied to quantify CYN in edible lettuce leaves exposed to CYN-contaminated water (10 µg L−1), showing that the tolerable daily intake (TDI) in the case of CYN could be exceeded in elderly high consumers. The validated method showed good results in terms of sensitivity, precision, accuracy, and robustness for CYN determination in leaf vegetables such as lettuce. More studies are needed in order to prevent the risks associated with the consumption of CYN-contaminated vegetables. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.MDPI20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/120503eng2072665110.3390/toxins10020063Prieto A.I.Guzmán-Guillén R.Díez-Quijada L.Campos A.Vasconcelos V.Jos Á.Cameán A.M.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T15:20:51Zoai:repositorio-aberto.up.pt:10216/120503Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:21:18.379928Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Validation of a method for cylindrospermopsin determination in vegetables: Application to real samples such as lettuce (lactuca sativa l.) |
title |
Validation of a method for cylindrospermopsin determination in vegetables: Application to real samples such as lettuce (lactuca sativa l.) |
spellingShingle |
Validation of a method for cylindrospermopsin determination in vegetables: Application to real samples such as lettuce (lactuca sativa l.) Prieto A.I. cylindrospermopsin bacterial toxin cylindrospermopsin uracil analytical parameters Article drug determination freeze drying lettuce limit of detection limit of quantitation linearity liquid chromatography-mass spectrometry measurement accuracy measurement precision nonhuman plant leaf risk assessment robustness ruggedness sensitivity analysis solvent extraction ultra performance liquid chromatography validation process vegetable analogs and derivatives analysis chemistry food contamination irrigation (agriculture) lettuce liquid chromatography procedures tandem mass spectrometry vegetable Agricultural Irrigation Bacterial Toxins Chromatography, Liquid Food Contamination Lettuce Limit of Detection Plant Leaves Tandem Mass Spectrometry Uracil Vegetables |
title_short |
Validation of a method for cylindrospermopsin determination in vegetables: Application to real samples such as lettuce (lactuca sativa l.) |
title_full |
Validation of a method for cylindrospermopsin determination in vegetables: Application to real samples such as lettuce (lactuca sativa l.) |
title_fullStr |
Validation of a method for cylindrospermopsin determination in vegetables: Application to real samples such as lettuce (lactuca sativa l.) |
title_full_unstemmed |
Validation of a method for cylindrospermopsin determination in vegetables: Application to real samples such as lettuce (lactuca sativa l.) |
title_sort |
Validation of a method for cylindrospermopsin determination in vegetables: Application to real samples such as lettuce (lactuca sativa l.) |
author |
Prieto A.I. |
author_facet |
Prieto A.I. Guzmán-Guillén R. Díez-Quijada L. Campos A. Vasconcelos V. Jos Á. Cameán A.M. |
author_role |
author |
author2 |
Guzmán-Guillén R. Díez-Quijada L. Campos A. Vasconcelos V. Jos Á. Cameán A.M. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Prieto A.I. Guzmán-Guillén R. Díez-Quijada L. Campos A. Vasconcelos V. Jos Á. Cameán A.M. |
dc.subject.por.fl_str_mv |
cylindrospermopsin bacterial toxin cylindrospermopsin uracil analytical parameters Article drug determination freeze drying lettuce limit of detection limit of quantitation linearity liquid chromatography-mass spectrometry measurement accuracy measurement precision nonhuman plant leaf risk assessment robustness ruggedness sensitivity analysis solvent extraction ultra performance liquid chromatography validation process vegetable analogs and derivatives analysis chemistry food contamination irrigation (agriculture) lettuce liquid chromatography procedures tandem mass spectrometry vegetable Agricultural Irrigation Bacterial Toxins Chromatography, Liquid Food Contamination Lettuce Limit of Detection Plant Leaves Tandem Mass Spectrometry Uracil Vegetables |
topic |
cylindrospermopsin bacterial toxin cylindrospermopsin uracil analytical parameters Article drug determination freeze drying lettuce limit of detection limit of quantitation linearity liquid chromatography-mass spectrometry measurement accuracy measurement precision nonhuman plant leaf risk assessment robustness ruggedness sensitivity analysis solvent extraction ultra performance liquid chromatography validation process vegetable analogs and derivatives analysis chemistry food contamination irrigation (agriculture) lettuce liquid chromatography procedures tandem mass spectrometry vegetable Agricultural Irrigation Bacterial Toxins Chromatography, Liquid Food Contamination Lettuce Limit of Detection Plant Leaves Tandem Mass Spectrometry Uracil Vegetables |
description |
Reports on the occurrence of the cyanobacterial toxin cylindrospermopsin (CYN) have increased worldwide because of CYN toxic effects in humans and animals. If contaminated waters are used for plant irrigation, these could represent a possible CYN exposure route for humans. For the first time, a method employing solid phase extraction and quantification by ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) of CYN was optimized in vegetables matrices such as lettuce (Lactuca sativa). The validated method showed a linear range, from 5 to 500 ng CYN g−1 of fresh weight (f.w.), and detection and quantitation limits (LOD and LOQ) of 0.22 and 0.42 ng CYN g−1 f.w., respectively. The mean recoveries ranged between 85 and 104%, and the intermediate precision from 12.7 to 14.7%. The method showed to be robust for the three different variables tested. Moreover, it was successfully applied to quantify CYN in edible lettuce leaves exposed to CYN-contaminated water (10 µg L−1), showing that the tolerable daily intake (TDI) in the case of CYN could be exceeded in elderly high consumers. The validated method showed good results in terms of sensitivity, precision, accuracy, and robustness for CYN determination in leaf vegetables such as lettuce. More studies are needed in order to prevent the risks associated with the consumption of CYN-contaminated vegetables. © 2018 by the authors. Licensee MDPI, Basel, Switzerland. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2018-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/10216/120503 |
url |
https://hdl.handle.net/10216/120503 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
20726651 10.3390/toxins10020063 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136129123876865 |