Monoolein as helper lipid for non-viral transfection in mammals

Detalhes bibliográficos
Autor(a) principal: Oliveira, Ana Cristina Norberto Gonçalves
Data de Publicação: 2010
Outros Autores: Silva, J. P. Neves, Coutinho, Paulo J. G., Gomes, A. A., Coutinho, O. P., Oliveira, M. Elisabete
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/14095
Resumo: Lipoplexes composed of pDNA and DODAB/MO at different molar ratios (4:1, 2:1 and 1:1) and different cationic lipid/DNA charge ratios were investigated. The physicochemical properties of the lipoplexes (size and charge), the pDNA complexation, and the effect of heparin on pDNA release, were studied by Dynamic Light Scattering, Zeta Potential, and Ethidium Bromide exclusion assays. The cytotoxicity, transfection efficiency and the intracellular localization of DNA were evaluated on 293T cells.
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spelling Monoolein as helper lipid for non-viral transfection in mammalsLipoplexesTransfectionDODABMonooleinScience & TechnologyLipoplexes composed of pDNA and DODAB/MO at different molar ratios (4:1, 2:1 and 1:1) and different cationic lipid/DNA charge ratios were investigated. The physicochemical properties of the lipoplexes (size and charge), the pDNA complexation, and the effect of heparin on pDNA release, were studied by Dynamic Light Scattering, Zeta Potential, and Ethidium Bromide exclusion assays. The cytotoxicity, transfection efficiency and the intracellular localization of DNA were evaluated on 293T cells.The Portuguese Foundation for Science and Technology (FCT) for the financial support to the Center of Physics and Center of Molecular & Environmental Biology and funding through projects PTDC/QUI/69795/2006 and SFRH/BD/46968/2009 are acknowledged.Elsevier Science BVUniversidade do MinhoOliveira, Ana Cristina Norberto GonçalvesSilva, J. P. NevesCoutinho, Paulo J. G.Gomes, A. A.Coutinho, O. P.Oliveira, M. Elisabete20102010-01-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/14095eng0168-365910.1016/j.jconrel.2010.07.067info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T07:07:44Zoai:repositorium.sdum.uminho.pt:1822/14095Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T07:07:44Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Monoolein as helper lipid for non-viral transfection in mammals
title Monoolein as helper lipid for non-viral transfection in mammals
spellingShingle Monoolein as helper lipid for non-viral transfection in mammals
Oliveira, Ana Cristina Norberto Gonçalves
Lipoplexes
Transfection
DODAB
Monoolein
Science & Technology
title_short Monoolein as helper lipid for non-viral transfection in mammals
title_full Monoolein as helper lipid for non-viral transfection in mammals
title_fullStr Monoolein as helper lipid for non-viral transfection in mammals
title_full_unstemmed Monoolein as helper lipid for non-viral transfection in mammals
title_sort Monoolein as helper lipid for non-viral transfection in mammals
author Oliveira, Ana Cristina Norberto Gonçalves
author_facet Oliveira, Ana Cristina Norberto Gonçalves
Silva, J. P. Neves
Coutinho, Paulo J. G.
Gomes, A. A.
Coutinho, O. P.
Oliveira, M. Elisabete
author_role author
author2 Silva, J. P. Neves
Coutinho, Paulo J. G.
Gomes, A. A.
Coutinho, O. P.
Oliveira, M. Elisabete
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Oliveira, Ana Cristina Norberto Gonçalves
Silva, J. P. Neves
Coutinho, Paulo J. G.
Gomes, A. A.
Coutinho, O. P.
Oliveira, M. Elisabete
dc.subject.por.fl_str_mv Lipoplexes
Transfection
DODAB
Monoolein
Science & Technology
topic Lipoplexes
Transfection
DODAB
Monoolein
Science & Technology
description Lipoplexes composed of pDNA and DODAB/MO at different molar ratios (4:1, 2:1 and 1:1) and different cationic lipid/DNA charge ratios were investigated. The physicochemical properties of the lipoplexes (size and charge), the pDNA complexation, and the effect of heparin on pDNA release, were studied by Dynamic Light Scattering, Zeta Potential, and Ethidium Bromide exclusion assays. The cytotoxicity, transfection efficiency and the intracellular localization of DNA were evaluated on 293T cells.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/14095
url http://hdl.handle.net/1822/14095
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0168-3659
10.1016/j.jconrel.2010.07.067
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier Science BV
publisher.none.fl_str_mv Elsevier Science BV
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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